Effect of 2000-meter Rowing Test on Parameters of Intestinal Integrity in Elite Rowers During Competitive Phase (Rowers)

The study aimed to check the 2000m ergometer test on markers of gut permeability in the competitive phase of rowers. 18 members of the Polish rowing team took part in the study.

Study Overview

• Status: Completed
• Conditions: Endothelial Dysfunction
• Intervention / Treatment: Other: ergometer test

Detailed Description

The phenomenon of epithelial wall leakage has been the subject of investigations within athletic domains such as running and cycling. Nevertheless, there exists an insufficiency of understanding regarding gut permeability in alternative disciplines, such as rowing, particularly following the completion of a standard competitive distance of 2000 meters. Hence, the principal objective of our study was to assess gut permeability after completing a 2000-meter rowing test. The study was performed at the beginning of a competitive training phase. Eighteen elite Polish Rowing Team rowers participated in the study after applying the inclusion/exclusion criteria. The participants performed a 2000-meter ergometer test. Blood samples were taken before the test, after exercise, and after 1-hour of restitution. Parameters, such as I-FABP, LPS, LBP, and zonulin, were determined using appropriate biochemical tests.

• Study Type: Observational
• Enrollment (Actual): 18

Contacts and Locations

Study Locations

• Poland
  • Poznań, Poland
    • Poznań University of Physical Education

Participation Criteria

Eligibility Criteria

• Ages Eligible for Study: Adult
• Accepts Healthy Volunteers: Yes

• Sampling Method: Non-Probability Sample

Study Population

Male rowers age 18 - 22 years.

Description

Inclusion Criteria:

• minimum five years of training,
• five training sessions per week minimum,
• total training time minimum of 240 minutes,
• membership in the Polish Rowing Team,
• finishing the 2000-meter ergometer test.

Exclusion Criteria:

• antibiotic therapy within the last three months,
• probiotics and prebiotics within the last three months,
• dietary regime,
• gastrointestinal diseases.

Study Plan

How is the study designed?

What is the study measuring?

Primary Outcome Measures

Outcome Measure	Measure Description	Time Frame
I-FABP (intestinal fatty acid binding protein)to measure epithelial wall injury	I-FABP concentration measured in blood [ng/ml] using commercially available enzyme-linked immunosorbent assays	Blood samples was taken from the cubital vein at three-time points: before each stress test (after overnight fasting), one minute after the end of the test, and after a 1-hour recovery period
zonulin to measure tight junction leakage	zonulin concentration measured in blood [ng/ml] using commercially available enzyme-linked immunosorbent assays	Blood samples was taken from the cubital vein at three-time points: before each stress test (after overnight fasting), one minute after the end of the test, and after a 1-hour recovery period
LBP (lipopolysaccharide binding protein) to measure endotoxin	LBP concentration measured in blood [ug/ml] using commercially available enzyme-linked immunosorbent assays	Blood samples was taken from the cubital vein at three-time points: before each stress test (after overnight fasting), one minute after the end of the test, and after a 1-hour recovery period
LPS (lipopolysaccharide) to measure endotoxin	LPS concentration measured in blood [EU/Ll] using commercially available enzyme-linked immunosorbent assays	Blood samples was taken from the cubital vein at three-time points: before each stress test (after overnight fasting), one minute after the end of the test, and after a 1-hour recovery period
Lactic acid to measure fatigue after the race	Lactate concentration measured in capillary blood from earlobe [mmol/L] using a commercially available kit (Diaglobal, Berlin, Germany)	Blood samples was taken from earlobe at two-time points: before each stress test (after overnight fasting), one minute after the end of the test.

Secondary Outcome Measures

Outcome Measure	Measure Description	Time Frame
energy	energy [kcal ]of food intake measured by 24h dietary recall	day before test
fiber intake	fiber [g] intake day before the test measured by 24h dietary recall	day before test
protein	protein [g] intake day before the test measured by 24h dietary recall	day before test
carbohydrate	carbohydrate [g] intake day before the test measured by 24h dietary recall	day before test
body mass	Body mass [kg] measured by electronic scale to the nearest 0,05 kg (Tanita BC 418 MA Tanita Corporation, Tokyo, Japan).	in the morning before the test
Body composition - water	% of body water measured by electronic scale (Tanita BC 418 MA Tanita Corporation, Tokyo, Japan).	in the morning before the test
Body composition - fat	% of body fat measured by electronic scale (Tanita BC 418 MA Tanita Corporation, Tokyo, Japan).	in the morning before the test
Lean body mass	Lean body mass [kg] measured by electronic scale (Tanita BC 418 MA Tanita Corporation, Tokyo, Japan).	in the morning before the test

Collaborators and Investigators

• Sponsor: Poznan University of Physical Education
• Investigators: Study Director: Anna Skarpańska-Stejnborn, proffessor, Poznań University of Physical Education

Study record dates

Study Major Dates

• Study Start (Actual): June 1, 2022
• Primary Completion (Actual): June 30, 2022
• Study Completion (Actual): June 30, 2022

Study Registration Dates

• First Submitted: March 19, 2024
• First Submitted That Met QC Criteria: April 12, 2024
• First Posted (Actual): April 17, 2024

Study Record Updates

• Last Update Posted (Actual): April 17, 2024
• Last Update Submitted That Met QC Criteria: April 12, 2024
• Last Verified: June 1, 2022

More Information

Terms related to this study

• Keywords: gut permeability; endotoxin; gastrointestinal symptoms
• Other Study ID Numbers: Rowers2022

Plan for Individual participant data (IPD)

• Plan to Share Individual Participant Data (IPD)?: NO

Drug and device information, study documents

• Studies a U.S. FDA-regulated drug product: No
• Studies a U.S. FDA-regulated device product: No