The Impact of Dietary Intervention on Oxidative/antioxidant Markers and Gut Microbiota in Athletes (SportDiet)

The Impact of Dietary Intervention Using Innovative Food with High Antioxidant Potential on Oxidative/antioxidant Markers in Blood and Gut Microbiota in Athletes

During intense physical exercise, there is an overproduction of reactive oxygen species, which leads to oxidative stress and reduced training and sports performance, as well as the development of chronic diseases. Eating foods with a high content of bioactive ingredients and high antioxidant potential can alleviate the negative effects caused by reactive oxygen species and improve the state of intestinal microflora.

The aim of these interventional studies was to determine whether daily consumption of foods with high antioxidant potential, including fruit and nut bars, for a period of 1 month would reduce oxidative stress in athletes during competition and positively change the intestinal microflora.

Study Overview

• Status: Completed
• Conditions: Non Communicable Diseases
• Intervention / Treatment: Other: Food with high antioxidant potential

Detailed Description

The study concerns a 4-week dietary intervention. The intervention consisted of daily consumption of 1 bar weighing 50 g, containing nuts and dried fruits with high antioxidant potential. The study included a group of 50 women and men, healthy athletes (cyclists, long-distance runners and triathletes). A 3-day, 24-hour dietary interview was conducted before and after the dietary intervention. Then, using the Dieta 6.0 computer program, the energy and nutrient contents in the diet were estimated. In addition, adherence to the dietary intervention was assessed using a survey questionnaire. Before and after the dietary intervention, oxidative-antioxidant markers were determined in the blood of the subjects (oxidative stress, antioxidant potential, concentration of antioxidant enzymes: glutathione peroxidase, superoxide dismutase, catalase) using ready-made kits. Moreover, changes in the intestinal microbiome were assessed in feces before and after the dietary intervention. Additionally, height and weight were measured before and after the intervention to calculate body mass index (BMI). The study will help answer the question of whether dietary intervention using foods with high antioxidant potential can influence changes in oxidative-antioxidant markers in the blood, intestinal microbiome and body weight of study participants.

• Study Type: Interventional
• Enrollment (Actual): 50
• Phase: Not Applicable

Contacts and Locations

Study Locations

• Poland
  • Białystok, Poland, 15-089
    • Medical University of Bialystok

Participation Criteria

Eligibility Criteria

• Ages Eligible for Study: Adult
• Accepts Healthy Volunteers: Yes

Description

Inclusion Criteria:

• healthy men and women with high physical activity (triathletes, cyclists, long-distance runners), not taking medications for chronic diseases, age 20-50 years, men and women.

Exclusion Criteria:

• occurence of chronic diseases (e.g. hypertension, diabetes, cancer, thyroid diseases, neurodegenerative diseases), taking medications for chronic diseases, age<20 and >50 years, low physical activity

Study Plan

How is the study designed?

Design Details

• Primary Purpose: Prevention
• Allocation: N/A
• Interventional Model: Single Group Assignment
• Masking: None (Open Label)

Number of Arms

1

Arms and Interventions

Participant Group / Arm	Intervention / Treatment
Experimental: Oxidative-antioxidant markers in blood and gut microbiota; Before and after the 1-month dietary intervention, the following markers will be determined in the blood: antioxidant potential, antioxidant enzymes, oxidative stress. Moreover, the intestinal microbiota will be determined before and after the intervention.	Other: Food with high antioxidant potential; The dietary intervention using fruit and nut bars with high antioxidant activity will last 1 month. Before and after the procedure, oxidative-antioxidant markers in the blood and intestinal microflora in the stool will be determined.

What is the study measuring?

Primary Outcome Measures

Outcome Measure	Measure Description	Time Frame
Change in antioxidant potential after dietary intervention	Before and after the dietary intervention, the antioxidant potential in the blood was determined using the ABTS method.	4 weeks
Change in gut microbiota after dietary intervention	The gut microbiota in feces was determined before and after the dietary intervention in a hospital laboratory.	4 weeks
Assessment of dietary intervention	The dietary intervention lasted 4 weeks. The subjects received one bar a day weighing 50 g, containing nuts and dried fruits with high antioxidant potential and high polyphenol content. After the dietary intervention, the participants completed a survey containing questions about compliance with the recommendations of the dietary intervention.	4 weeks
Change in blood glutathione peroxidase concentration after dietary intervention	Before and after the dietary intervention, the concentration of glutathione peroxidase enzyme in blood was assessed using ready-made laboratory kits.	4 weeks
Change in superoxide dismutase concentration after dietary intervention	Before and after the dietary intervention, the concentration of superoxide dismutase enzyme in blood was assessed using ready-made laboratory kits.	4 weeks
Change in catalase concentrations after dietary intervention	Before and after the dietary intervention, the concentration of catalase enzyme in blood was assessed using ready-made laboratory kits.	4 weeks
Change in oxidative stress after dietary intervention	Before and after the dietary intervention, the oxidative stress in blood was assessed using ready-made laboratory kits.	4 weeks

Secondary Outcome Measures

Outcome Measure	Measure Description	Time Frame
Assessment of dietary nutrients before and after dietary intervention	Before and after the dietary intervention, a 3-day, 24-hour interview was conducted regarding the consumption of products and dishes during the day. Using the Diet 6.0 computer program, the content of individual nutrients in the diet and energy of diet were determined.	4 weeks
Body weight assessment before and after dietary intervention	The change in body weight of the subjects before and after the dietary intervention was determined using a scale in kilograms with an accuracy of 100 g.	4 weeks
Measuring the height of study participants	Height was determined in centimeters using a measuring tape with an accuracy of 1 cm.	4 weeks

Collaborators and Investigators

• Sponsor: Medical University of Bialystok
• Investigators: Principal Investigator: Malgorzata E Zujko, Prof., Medical University of Bialystok, Jana Kilinskiego 1, 15-089 Bialystok

Study record dates

Study Major Dates

• Study Start (Actual): January 15, 2025
• Primary Completion (Actual): February 15, 2025
• Study Completion (Actual): February 15, 2025

Study Registration Dates

• First Submitted: February 20, 2025
• First Submitted That Met QC Criteria: February 25, 2025
• First Posted (Actual): March 25, 2025

Study Record Updates

• Last Update Posted (Actual): March 25, 2025
• Last Update Submitted That Met QC Criteria: February 25, 2025
• Last Verified: February 1, 2025

More Information

Terms related to this study

• Keywords: cardiovascular disease; sport; antioxidant stress
• Additional Relevant MeSH Terms: Pathologic Processes; Disease Attributes; Noncommunicable Diseases; Physiological Effects of Drugs; Molecular Mechanisms of Pharmacological Action; Protective Agents; Antioxidants
• Other Study ID Numbers: APK.002.405.2024; SKN/SP/601112/2024 (Other Grant/Funding Number: Ministry of Science and Higher Education in Poland); B.SUB.25.207 (Other Grant/Funding Number: Medical University of Bialystok)

Plan for Individual participant data (IPD)

• Plan to Share Individual Participant Data (IPD)?: YES
• IPD Plan Description: The research results will be published in a scientific journal. Detailed data of respondents are not made available due to personal data protection.
• IPD Sharing Time Frame: The clinical trial report will be published in scientific journals after the study is completed.
• IPD Sharing Access Criteria: Additional information can be obtained by writing to the principal investigator's address: malgorzata.zujko@umb.edu.pl.
• IPD Sharing Supporting Information Type: CSR

Drug and device information, study documents

• Studies a U.S. FDA-regulated drug product: No
• Studies a U.S. FDA-regulated device product: No
• product manufactured in and exported from the U.S.: No