The Effects of One Day of Activity on the Next Day's Exercise Response

February 20, 2026 updated by: Concordia University Wisconsin

A Single Day of Inactivity on the Systemic Metabolic and Inflammatory Exercise Response

Exercise can reduce the risk of several chronic diseases. However, recent studies have suggested sitting for too long before exercising can decrease exercise benefits. This study will help determine why this may occur.

Study Overview

Status

Recruiting

Conditions

Intervention / Treatment

Detailed Description

Previous work demonstrated a greater utilization of carbohydrates (CHO) at the end of aerobic exercise with a day of reduced activity (<5,000 steps). Additionally, there are differences in the RNA profile at rest and during exercise in pathways related to inflammation, metabolism, and innate immunity that warrant further research. Together, these data highlight that a single day of reduced activity alters systemic homeostasis that affects the exercise response. Given the potency of exercise to ameliorate risk of cardiovascular disease, more research is needed to find factors to augment the exercise response. Therefore, this study has two goals: 1) Analyze circulating metabolomics, and 2) metabolomics and proteomics in response to exercise after a day of reduced activity.

Study Type

Interventional

Enrollment (Estimated)

6

Phase

  • Not Applicable

Contacts and Locations

This section provides the contact details for those conducting the study, and information on where this study is being conducted.

Study Contact

Study Locations

  • United States
    • Wisconsin
      • Mequon, Wisconsin, United States, 53093
        • Recruiting
        • Concordia University of Wisconsin
        • Contact:

Participation Criteria

Researchers look for people who fit a certain description, called eligibility criteria. Some examples of these criteria are a person's general health condition or prior treatments.

Eligibility Criteria

Ages Eligible for Study

  • Adult

Accepts Healthy Volunteers

Yes

Description

Inclusion Criteria:

  • • between the ages of 18-30 years old

Exclusion Criteria:

  • • free from acute or chronic illness (cardiac, pulmonary, liver, or kidney abnormalities, cancer, uncontrolled hypertension, insulin- or non-insulin dependent diabetes or other known metabolic disorders)

    • do not take medications related to metabolism (i.e., statins, metformin, GLP-1 agonists)
    • free from orthopedic limitations (including any artificial joints)
    • currently smoke or participate in other forms of tobacco use.

Study Plan

This section provides details of the study plan, including how the study is designed and what the study is measuring.

How is the study designed?

Design Details

  • Primary Purpose: Prevention
  • Allocation: Randomized
  • Interventional Model: Crossover Assignment
  • Masking: None (Open Label)

Arms and Interventions

Participant Group / Arm
Intervention / Treatment
Active Comparator: >8,500 steps the day prior to exericse
Behavioral: Decreased step count
Subjects will walk less than 5,000 steps
Experimental: <5,000 steps the day prior to exercise
Behavioral: Decreased step count
Subjects will walk less than 5,000 steps

What is the study measuring?

Primary Outcome Measures

Outcome Measure
Measure Description
Time Frame
Concentration of metabolites within the plasma
Time Frame: Participants will complete the study within 6 weeks of enrollment.
We will collect blood before and immediately after exercise. Plasma will be separated and sent for untargeted metabolomic analysis.
Participants will complete the study within 6 weeks of enrollment.
Concentration of proteins within the plasma
Time Frame: Participants will complete the study within 6 weeks of enrollment.
We will collect blood before and immediately after exercise. Plasma will be separated and sent for untargeted proteomic analysis.
Participants will complete the study within 6 weeks of enrollment.

Collaborators and Investigators

This is where you will find people and organizations involved with this study.

Sponsor

Concordia University Wisconsin

Study record dates

These dates track the progress of study record and summary results submissions to ClinicalTrials.gov. Study records and reported results are reviewed by the National Library of Medicine (NLM) to make sure they meet specific quality control standards before being posted on the public website.

Study Major Dates

Study Start (Actual)

January 1, 2026

Primary Completion (Estimated)

December 31, 2026

Study Completion (Estimated)

December 31, 2026

Study Registration Dates

First Submitted

February 17, 2026

First Submitted That Met QC Criteria

February 20, 2026

First Posted (Actual)

February 24, 2026

Study Record Updates

Last Update Posted (Actual)

February 24, 2026

Last Update Submitted That Met QC Criteria

February 20, 2026

Last Verified

February 1, 2026

More Information

Terms related to this study

Keywords

Additional Relevant MeSH Terms

Other Study ID Numbers

  • https://pubmed.ncbi.nlm.nih.go
  • 26BAIREA1560979 (Other Identifier: American Heart Association)

Plan for Individual participant data (IPD)

Plan to Share Individual Participant Data (IPD)?

YES

Drug and device information, study documents

Studies a U.S. FDA-regulated drug product

No

Studies a U.S. FDA-regulated device product

No

This information was retrieved directly from the website clinicaltrials.gov without any changes. If you have any requests to change, remove or update your study details, please contact register@clinicaltrials.gov. As soon as a change is implemented on clinicaltrials.gov, this will be updated automatically on our website as well.

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