Comparison of the Effects of Kinesio Taping and Graston Application in Individuals With Delayed Onset Muscle Soreness

August 11, 2026 updated by: Mustafa Can Salamci, Gazi University

Comparison of the Effects of Kinesio Taping and the Graston Technique on Pain, Muscle Oxygen Saturation, Peak Squat Force, and Lower-Extremity Response Time in Individuals With Delayed-Onset Muscle Soreness

Delayed-onset muscle soreness (DOMS) is characterized by pain, stiffness, and discomfort that typically develop 24-72 hours after unaccustomed or intense physical activity. Previous studies suggest that both Kinesio taping and the Graston technique may help reduce DOMS-related symptoms. However, these two approaches have not been directly compared, and their effects on muscle oxygen saturation, peak squat force, and lower-extremity response time remain unclear. Therefore, the aim of this study was to compare the short-term effects of Kinesio taping and the Graston technique in healthy participants with exercise-induced DOMS.

A total of 36 participants were randomly allocated to three groups: a Kinesio taping group, a Graston technique group, and a control group that received no therapeutic intervention and recovered naturally. Pain severity, muscle oxygen saturation, peak squat force, and lower-extremity response time were assessed 0h after the DOMS-induction protocol, before the interventions, and again 48 hours after the DOMS-induction protocol, corresponding to 48 hours after the interventions were initiated.

Study Overview

Detailed Description

Delayed-onset muscle soreness (DOMS) is characterized by pain, discomfort, and stiffness that typically develop within 24-72 hours after unaccustomed or intense physical activity. Previous studies suggest that both Kinesio taping (KT) and the Graston technique may reduce DOMS-related symptoms; however, these approaches have not been directly compared, and their effects on muscle oxygen saturation, functional muscle force, and lower-extremity response time remain unclear. The aim of this study was to compare the short-term effects of Kinesio taping and the Graston technique on pain severity, muscle oxygen saturation, functional muscle force, and lower-extremity response time in individuals with exercise-induced DOMS.

A total of 36 healthy participants were randomly allocated to three groups: a Kinesio taping group, a Graston technique group, and a control group that received no therapeutic intervention and recovered naturally. Pain severity, muscle oxygen saturation, peak squat force, and lower-extremity response time were assessed 0hours after the DOMS-induction protocol, before initiation of the interventions, and again 48hours after the DOMS-induction protocol. The changes in these outcomes were statistically compared among the groups.

Study Type

Interventional

Enrollment (Actual)

36

Phase

  • Not Applicable

Contacts and Locations

This section provides the contact details for those conducting the study, and information on where this study is being conducted.

Study Locations

    • Please Select
      • Ankara, Please Select, Turkey (Türkiye), 06490
        • Gazi University

Participation Criteria

Researchers look for people who fit a certain description, called eligibility criteria. Some examples of these criteria are a person's general health condition or prior treatments.

Eligibility Criteria

Ages Eligible for Study

  • Adult

Accepts Healthy Volunteers

Yes

Description

Inclusion Criteria:

  • Being between 18 and 30 years of age
  • Being classified as inactive according to the International Physical Activity Questionnaire
  • Having a body mass index below 25

Exclusion Criteria:

  • Presence of a chronic disease
  • History of lower extremity orthopedic disorders or surgery
  • Current lower extremity muscle pain
  • History of lower extremity trauma within the last month
  • Use of medications that may affect the musculoskeletal system (e.g., muscle relaxants, anti-inflammatory drugs)
  • Caffeine consumption within 24 hours before exercise
  • Participation in another exercise or intense physical activity within 24 hours before the test
  • Use of performance-enhancing supplements

Study Plan

This section provides details of the study plan, including how the study is designed and what the study is measuring.

How is the study designed?

Design Details

  • Primary Purpose: Treatment
  • Allocation: Randomized
  • Interventional Model: Parallel Assignment
  • Masking: Single

Arms and Interventions

Participant Group / Arm
Intervention / Treatment
Active Comparator: Kinesio Taping
The group of individuals who will undergo kinesio taping application.
In this study, a lymphatic Kinesio taping technique was applied over the quadriceps femoris muscle. Two 5-cm-wide Kinesio tapes were cut to an appropriate length according to the dimensions of the target area. Each tape was prepared in a fan-cut configuration consisting of an uncut base and five longitudinal tails. The base was applied proximally near the inguinal lymph nodes without tension. The five tails were then applied over the quadriceps femoris with approximately 15% tension, while the terminal portions of the tails were applied without tension. The application was performed with the participant in the supine position and the quadriceps muscle relaxed.
Experimental: Graston Technique
The group of individuals who will undergo Graston technique application.
The Graston technique, a form of instrument-assisted soft tissue mobilization, was applied using a stainless-steel instrument and baby oil as a lubricant. Participants were positioned supine with the quadriceps femoris relaxed. The convex surface of the instrument was applied sequentially over the vastus lateralis, rectus femoris, and vastus medialis muscles in a cranial-to-caudal direction. Each muscle was treated for 2 minutes, resulting in a total treatment duration of 6 minutes per session. The first session was performed immediately after the post-DOMS pre-intervention assessment, and the second session was performed 24 hours later.
No Intervention: Control
spontaneous recovery process

What is the study measuring?

Primary Outcome Measures

Outcome Measure
Measure Description
Time Frame
Visual Analog Scale
Time Frame: 48 hours
Pain severity was assessed using a 10-cm horizontal Visual Analog Scale (VAS), anchored by 0 ("no pain") and 10 ("worst imaginable pain"). Participants were asked to mark the point on the line that best represented their perceived pain intensity. The distance from the 0 anchor to the marked point was measured in centimetres using a ruler, with higher scores indicating greater pain severity. Pain was assessed separately at rest and during the squat task.
48 hours

Secondary Outcome Measures

Outcome Measure
Measure Description
Time Frame
Peak Squat Force
Time Frame: 48 hours
Peak squat force was assessed using the K-Force Plates (K-Invent®, France). Participants performed single-leg squats on the exercised dominant lower extremity to approximately 90-100° of knee flexion. Three 10-second trials were performed, with a 60-second rest period between trials. Participants were instructed to perform as many squat repetitions as possible while maintaining proper technique. Peak squat force recorded during the task was used for analysis.
48 hours
Response Time
Time Frame: 48 hours
Lower-extremity response time was assessed using the BlazePod™ system (Play Coyotta Ltd., Tel Aviv, Israel), a light-based visual stimulus system with reported test-retest reliability for response-time assessment. Participants performed the test in a seated position. Following a visual light stimulus, they were instructed to touch the illuminated pod as quickly as possible using the exercised lower extremity. The time between presentation of the visual stimulus and contact with the pod was recorded by the system. Mean response time, expressed in milliseconds (ms), was used for analysis, with lower values indicating faster responses.
48 hours
Muscle Oxygen Saturation (SmO₂)
Time Frame: 48 hours
Muscle oxygen saturation (SmO₂) was measured using a wearable near-infrared spectroscopy (NIRS) device (Train.Red, The Netherlands). The sensor was positioned over the rectus femoris muscle of the dominant lower extremity and secured using the strap provided with the device. The sensor position was standardized across assessments to ensure consistent placement. Muscle oxygen saturation was recorded during a 30-second unilateral squat task, and the mean SmO₂ (%) value obtained during the task was used for analysis.
48 hours

Collaborators and Investigators

This is where you will find people and organizations involved with this study.

Sponsor

Publications and helpful links

The person responsible for entering information about the study voluntarily provides these publications. These may be about anything related to the study.

Study record dates

These dates track the progress of study record and summary results submissions to ClinicalTrials.gov. Study records and reported results are reviewed by the National Library of Medicine (NLM) to make sure they meet specific quality control standards before being posted on the public website.

Study Major Dates

Study Start (Actual)

June 20, 2024

Primary Completion (Actual)

June 1, 2025

Study Completion (Actual)

June 1, 2025

Study Registration Dates

First Submitted

February 18, 2026

First Submitted That Met QC Criteria

February 23, 2026

First Posted (Actual)

February 27, 2026

Study Record Updates

Last Update Posted (Actual)

August 13, 2026

Last Update Submitted That Met QC Criteria

August 11, 2026

Last Verified

August 1, 2026

More Information

Terms related to this study

Other Study ID Numbers

  • 2024 - 635

Plan for Individual participant data (IPD)

Plan to Share Individual Participant Data (IPD)?

UNDECIDED

Drug and device information, study documents

Studies a U.S. FDA-regulated drug product

No

Studies a U.S. FDA-regulated device product

No

This information was retrieved directly from the website clinicaltrials.gov without any changes. If you have any requests to change, remove or update your study details, please contact register@clinicaltrials.gov. As soon as a change is implemented on clinicaltrials.gov, this will be updated automatically on our website as well.

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