Cutaneous DNA Damage Caused by UV-A Irradiation (DIMUVA)
Damage to DNA Caused by UV-A Irradiation: Photochemical Mechanism and Cutaneous Parameters Involved in the Formation of Cyclobutane Pyrimidine Dimers
調査の概要
詳細な説明
Due to their capacity to damage Deoxyribonucleic acid (DNA), Ultra-Violet (UV) radiation is one of the causes of skin cancers.
Until recently, the genotoxic effects of UV-A radiation, were poorly identified, in particular their capacity to lead to the dimerization of pyrimidine bases .
It is well known that the response to UV-A and UV-B radiations is different depending on the cutaneous phototype.
Thus, the aim of this study is to determine the correlation between cutaneous phototype and the quantity and nature (CPD or oxidative lesions) of damage caused to cutaneous DNA after an ex-vivo exposure to UV-A and UV-B radiations.
研究の種類
入学 (予想される)
段階
- 適用できない
連絡先と場所
研究場所
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Grenoble、フランス、38043
- Centre d'investigation Clinique ,University Hospital Grenoble
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Grenoble、フランス、38043
- Department of Dermatology, University Hospital Grenoble
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参加基準
適格基準
就学可能な年齢
健康ボランティアの受け入れ
受講資格のある性別
説明
Inclusion Criteria:
- Male,
- Between 18 and 35 years old,
- Healthy volunteers,
- Cutaneous phototype 2 or 4 according to the Fitzpatrick classification,
- Affiliation to the French Social Security.
Exclusion Criteria:
- History of photosensibility,
- Active smoking or stopped since less than one year,
- Dermatological pathology or treatment contra-indicating cutaneous irradiation and skin biopsies,
- Any chronic pathology susceptible to interfere with the evaluations related to the protocol,
- Allergy to local anaesthetics,
- Volunteers who take drugs and/or food complements acting on oxidative stress in the 8 weeks preceding inclusion,
- Volunteers who have take paracetamol or aspirin within 7 days prior to the inclusion visit,
- Subject in exclusion period for another biomedical research study,
- Subject having exceeded the threshold of annual compensation for biomedical research.
研究計画
研究はどのように設計されていますか?
デザインの詳細
- 主な目的:ヘルスサービス研究
- 割り当て:非ランダム化
- 介入モデル:並列代入
- マスキング:なし(オープンラベル)
武器と介入
参加者グループ / アーム |
介入・治療 |
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実験的:phototype 2
Volunteers with cutaneous phototype 2
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実験的:phototype 4
Volunteers with cutaneous phototype 4
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この研究は何を測定していますか?
主要な結果の測定
結果測定 |
時間枠 |
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Phototype determination according to the Fitzpatrick classification Number of CPD and oxidative lesions determinated by the analysis of DNA from the skin biopsies after their ex-vivo exposure to UV-A - The CPD / Oxidative lesions ratio
時間枠:Day 0
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Day 0
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二次結果の測定
結果測定 |
時間枠 |
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Number of CPD and oxidative lesions determinated by the analysis of DNA from the skin biopsies after their exposure ex-vivo to UV-B.
時間枠:Day 0
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Day 0
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UV-A radiation exposure: Minimal erythemic dose - Number of CPD and oxidative lesions - CPD / oxidative lesions ratio
時間枠:Day x
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Day x
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UV-B radiation exposure: Minimal erythemic dose - Number of CPD and oxidative lesions - CPD/oxidative lesions ratio
時間枠:Day 0
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Day 0
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antioxidant status and quantity of CPD, oxidative lesions after exposure to UV-A and UV-B radiations
時間枠:day 2
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day 2
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協力者と研究者
捜査官
- 主任研究者:Jean-Claude BEANI, Pr、University Hospital, Grenoble
出版物と役立つリンク
一般刊行物
- Brash DE. Sunlight and the onset of skin cancer. Trends Genet. 1997 Oct;13(10):410-4. doi: 10.1016/s0168-9525(97)01246-8.
- Melnikova VO, Ananthaswamy HN. Cellular and molecular events leading to the development of skin cancer. Mutat Res. 2005 Apr 1;571(1-2):91-106. doi: 10.1016/j.mrfmmm.2004.11.015.
- Cadet J, Sage E, Douki T. Ultraviolet radiation-mediated damage to cellular DNA. Mutat Res. 2005 Apr 1;571(1-2):3-17. doi: 10.1016/j.mrfmmm.2004.09.012. Epub 2005 Jan 26.
- Douki T, Reynaud-Angelin A, Cadet J, Sage E. Bipyrimidine photoproducts rather than oxidative lesions are the main type of DNA damage involved in the genotoxic effect of solar UVA radiation. Biochemistry. 2003 Aug 5;42(30):9221-6. doi: 10.1021/bi034593c.
- Dumaz N, Drougard C, Sarasin A, Daya-Grosjean L. Specific UV-induced mutation spectrum in the p53 gene of skin tumors from DNA-repair-deficient xeroderma pigmentosum patients. Proc Natl Acad Sci U S A. 1993 Nov 15;90(22):10529-33. doi: 10.1073/pnas.90.22.10529.
研究記録日
主要日程の研究
研究開始
一次修了 (実際)
研究の完了 (実際)
試験登録日
最初に提出
QC基準を満たした最初の提出物
最初の投稿 (見積もり)
学習記録の更新
投稿された最後の更新 (見積もり)
QC基準を満たした最後の更新が送信されました
最終確認日
詳しくは
本研究に関する用語
キーワード
その他の研究ID番号
- DCIC 08 13
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UVA and UVB irradiationの臨床試験
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Stiefel, a GSK CompanyGlaxoSmithKline完了