The Effects of Obesity on Glutathione Levels in Patients With Chronic Periodontitis Before and After Periodontal Therapy
Phase 1 Study of Periodontal Disease and Obese Individuals That Investigate of Local and Systemic Antioxidant Levels of Before and After Periodontal Treatment
調査の概要
詳細な説明
The purpose of this study was to investigate the effects of obesity on glutathione levels in the plasma, GCF and saliva of patients with chronic periodontitis and to evaluate changes after nonsurgical periodontal therapy.
Diagnosed as obese (n=30) and normal-weight (n=30) individuals were categorized; chronic periodontitis (CP) and periodontally healthy controls (PH). Gingival crevicular fluid (GCF), plasma, saliva samples and clinical measurements were obtained at baseline and a month after nonsurgical periodontal therapy.
Unstimulated salivary samples were collected using standard techniques. About 2 mL whole saliva was collected in disposable tubes and centrifuged immediately to remove cell debris (10,000 g x 10 minutes). The supernatants (50µL each) were stored at -40C until analyzed. GCF samples were collected from a mesio-buccal and disto-palatal site on each tooth. In the CP group, the samples were obtained from patients at areas with ≥5 mm CAL, ≥6 mm PD and ≥30% bone loss. In gingivitis group, GCF samples were obtained from teeth with BOP and without CAL. In the healthy group, GCF samples were collected from teeth exhibiting PD<3 mm without CAL and BOP. The area was isolated with cotton rolls, saliva contamination elimination was ensured, and it was slightly air dried. GCF was sampled with paper strips. Paper strips were placed into the crevice until mild resistance was felt (intracrevicular method) and left in the position for 30 seconds. Strips contaminated with blood or saliva were discarded. Each sampled strip was placed into a 400µl eppendorf centrifuge tube and stored at -40C until analyzed.
Five milliliters of venous blood was taken from antecubital vein by using a standard venipuncture method. Obtained blood sample was collected in vacutainer tubes and anti-coagulated with EDTA. The blood samples were then stored at -40C until required for use in ELISAs.
Power analysis indicated that 12 individuals for each group would be sufficient to achieve 80% power to detect a difference of 0.05 between the alternative and the null hypotheses.
The Shapiro Wilk test was used to investigate whether or not the data were normally distributed. Continuous variables with unequal variances were compared by means of Welch and Tamhane's T2 post-hoc test for BMI, PD, CAL and the levels of glutathione. The comparison of the age, GI, PI and BOP was analyzed using the Kruskal-Wallis non-parametric test followed by post hoc group comparisons with the Bonferroni-adjusted Mann-Whitney U test. Paired Student's t-test or Wilcoxon rank-sum test was used to compare the measurements at two points (baseline and after SRP). The Spearman's rank correlation test was also used to detect the relationship between biochemical and clinical findings.
研究の種類
入学 (実際)
段階
- フェーズ 1
参加基準
適格基準
就学可能な年齢
健康ボランティアの受け入れ
受講資格のある性別
説明
Inclusion Criteria:
- never-smokers
- no history of systemic disease
- no patients had been under periodontal therapy and medicine for at least 6 months before the study
- no pregnancy or lactation
- no alcohol or antioxidant vitamin consumption.
Exclusion Criteria:
-
研究計画
研究はどのように設計されていますか?
デザインの詳細
- 主な目的:診断
- 割り当て:ランダム化
- 介入モデル:並列代入
- マスキング:4倍
武器と介入
参加者グループ / アーム |
介入・治療 |
---|---|
アクティブコンパレータ:obese-chronic periodontitis patients
GCF, plasma and GCF samples were taken before and after nonsurgical periodontal therapy
|
Scaling and root planning under local anaesthesia, in a total of four clinical visits Oral hygiene instructions including the modified Bass technique, regular toothpaste, and an appropriate interdental cleaning device with dental floss and interdental brush.
他の名前:
|
プラセボコンパレーター:obese-periodontally healthy controls
GCF, plasma and GCF samples were taken at baseline after oral hygiene instructions
|
Oral hygiene instructions including the modified Bass technique, regular toothpaste, and an appropriate interdental cleaning device with dental floss and interdental brush
他の名前:
|
アクティブコンパレータ:normal weight-CP patients
GCF, plasma and GCF samples were taken before and after nonsurgical periodontal therapy
|
Scaling and root planning under local anaesthesia, in a total of four clinical visits Oral hygiene instructions including the modified Bass technique, regular toothpaste, and an appropriate interdental cleaning device with dental floss and interdental brush.
他の名前:
|
プラセボコンパレーター:normal weight-PH controls
GCF, plasma and GCF samples were taken at baseline after oral hygiene instructions
|
Oral hygiene instructions including the modified Bass technique, regular toothpaste, and an appropriate interdental cleaning device with dental floss and interdental brush
他の名前:
|
この研究は何を測定していますか?
主要な結果の測定
結果測定 |
メジャーの説明 |
時間枠 |
---|---|---|
Biochemical parameters (GSH and GSSG levels, GSH/GSSG ratio )
時間枠:Baseline and 1 month after treatment
|
The changes in levels of reduced and oxidized glutathione (GSH and GSSG) 1 month after periodontal treatment determined by ELISA.
The changes in levels of GSH were analyzed to determine diagnostic and prognostic potential as a biomarker of periodontitis and obesity.
|
Baseline and 1 month after treatment
|
二次結果の測定
結果測定 |
メジャーの説明 |
時間枠 |
---|---|---|
ポケットの深さを調べる
時間枠:ベースラインと治療後 1 か月
|
歯周治療後のプロービングポケットの深さの変化。疾患の重症度と臨床転帰を決定するためにプロービングポケットの深さを測定しました。
|
ベースラインと治療後 1 か月
|
ポケットの深さと臨床的付着レベルの調査
時間枠:ベースラインと治療後 1 か月
|
歯周治療後の臨床的愛着レベルの変化。
プロービングの深さと歯肉縁からセメントとエナメル質の接合部までの距離は、臨床的付着レベルの測定に使用されます。
疾患の重症度および臨床転帰を決定するために、臨床的愛着レベルが測定されました。
|
ベースラインと治療後 1 か月
|
プラークインデックス
時間枠:ベースラインと治療後 1 か月
|
歯周治療後のプラーク指数の変化。
歯垢指数は、口腔衛生状態を判定および分類するために記録されました。
|
ベースラインと治療後 1 か月
|
プロービング時の出血
時間枠:ベースラインと治療後 1 か月
|
歯周治療後のプロービング時の出血の変化。
プロービング時の出血は、(根尖部の)歯肉炎症を分類および評価するために記録されました。
|
ベースラインと治療後 1 か月
|
Gingival index
時間枠:Baseline and 1 month after treatment
|
he changes in gingival index after periodontal treatment.
Gingival index was recorded for classifying and evaluating (coronally) gingival inflammation.
|
Baseline and 1 month after treatment
|
協力者と研究者
スポンサー
研究記録日
主要日程の研究
研究開始
一次修了 (実際)
研究の完了 (実際)
試験登録日
最初に提出
QC基準を満たした最初の提出物
最初の投稿 (見積もり)
学習記録の更新
投稿された最後の更新 (見積もり)
QC基準を満たした最後の更新が送信されました
最終確認日
詳しくは
この情報は、Web サイト clinicaltrials.gov から変更なしで直接取得したものです。研究の詳細を変更、削除、または更新するリクエストがある場合は、register@clinicaltrials.gov。 までご連絡ください。 clinicaltrials.gov に変更が加えられるとすぐに、ウェブサイトでも自動的に更新されます。
nonsurgical periodontal therapyの臨床試験
-
Universitätsklinikum Hamburg-EppendorfCytoSorbents, Inc募集
-
Universitätsklinikum Hamburg-Eppendorf完了
-
Advanced Cooling Therapy, Inc., d/b/a Attune MedicalDnipropetrovsk State Medical Academy完了