- ICH GCP
- 미국 임상 시험 레지스트리
- 임상시험 NCT02498561
The Effects of Obesity on Glutathione Levels in Patients With Chronic Periodontitis Before and After Periodontal Therapy
Phase 1 Study of Periodontal Disease and Obese Individuals That Investigate of Local and Systemic Antioxidant Levels of Before and After Periodontal Treatment
연구 개요
상세 설명
The purpose of this study was to investigate the effects of obesity on glutathione levels in the plasma, GCF and saliva of patients with chronic periodontitis and to evaluate changes after nonsurgical periodontal therapy.
Diagnosed as obese (n=30) and normal-weight (n=30) individuals were categorized; chronic periodontitis (CP) and periodontally healthy controls (PH). Gingival crevicular fluid (GCF), plasma, saliva samples and clinical measurements were obtained at baseline and a month after nonsurgical periodontal therapy.
Unstimulated salivary samples were collected using standard techniques. About 2 mL whole saliva was collected in disposable tubes and centrifuged immediately to remove cell debris (10,000 g x 10 minutes). The supernatants (50µL each) were stored at -40C until analyzed. GCF samples were collected from a mesio-buccal and disto-palatal site on each tooth. In the CP group, the samples were obtained from patients at areas with ≥5 mm CAL, ≥6 mm PD and ≥30% bone loss. In gingivitis group, GCF samples were obtained from teeth with BOP and without CAL. In the healthy group, GCF samples were collected from teeth exhibiting PD<3 mm without CAL and BOP. The area was isolated with cotton rolls, saliva contamination elimination was ensured, and it was slightly air dried. GCF was sampled with paper strips. Paper strips were placed into the crevice until mild resistance was felt (intracrevicular method) and left in the position for 30 seconds. Strips contaminated with blood or saliva were discarded. Each sampled strip was placed into a 400µl eppendorf centrifuge tube and stored at -40C until analyzed.
Five milliliters of venous blood was taken from antecubital vein by using a standard venipuncture method. Obtained blood sample was collected in vacutainer tubes and anti-coagulated with EDTA. The blood samples were then stored at -40C until required for use in ELISAs.
Power analysis indicated that 12 individuals for each group would be sufficient to achieve 80% power to detect a difference of 0.05 between the alternative and the null hypotheses.
The Shapiro Wilk test was used to investigate whether or not the data were normally distributed. Continuous variables with unequal variances were compared by means of Welch and Tamhane's T2 post-hoc test for BMI, PD, CAL and the levels of glutathione. The comparison of the age, GI, PI and BOP was analyzed using the Kruskal-Wallis non-parametric test followed by post hoc group comparisons with the Bonferroni-adjusted Mann-Whitney U test. Paired Student's t-test or Wilcoxon rank-sum test was used to compare the measurements at two points (baseline and after SRP). The Spearman's rank correlation test was also used to detect the relationship between biochemical and clinical findings.
연구 유형
등록 (실제)
단계
- 1단계
참여기준
자격 기준
공부할 수 있는 나이
건강한 자원 봉사자를 받아들입니다
연구 대상 성별
설명
Inclusion Criteria:
- never-smokers
- no history of systemic disease
- no patients had been under periodontal therapy and medicine for at least 6 months before the study
- no pregnancy or lactation
- no alcohol or antioxidant vitamin consumption.
Exclusion Criteria:
-
공부 계획
연구는 어떻게 설계됩니까?
디자인 세부사항
- 주 목적: 특수 증상
- 할당: 무작위
- 중재 모델: 병렬 할당
- 마스킹: 네 배로
무기와 개입
참가자 그룹 / 팔 |
개입 / 치료 |
---|---|
활성 비교기: obese-chronic periodontitis patients
GCF, plasma and GCF samples were taken before and after nonsurgical periodontal therapy
|
Scaling and root planning under local anaesthesia, in a total of four clinical visits Oral hygiene instructions including the modified Bass technique, regular toothpaste, and an appropriate interdental cleaning device with dental floss and interdental brush.
다른 이름들:
|
위약 비교기: obese-periodontally healthy controls
GCF, plasma and GCF samples were taken at baseline after oral hygiene instructions
|
Oral hygiene instructions including the modified Bass technique, regular toothpaste, and an appropriate interdental cleaning device with dental floss and interdental brush
다른 이름들:
|
활성 비교기: normal weight-CP patients
GCF, plasma and GCF samples were taken before and after nonsurgical periodontal therapy
|
Scaling and root planning under local anaesthesia, in a total of four clinical visits Oral hygiene instructions including the modified Bass technique, regular toothpaste, and an appropriate interdental cleaning device with dental floss and interdental brush.
다른 이름들:
|
위약 비교기: normal weight-PH controls
GCF, plasma and GCF samples were taken at baseline after oral hygiene instructions
|
Oral hygiene instructions including the modified Bass technique, regular toothpaste, and an appropriate interdental cleaning device with dental floss and interdental brush
다른 이름들:
|
연구는 무엇을 측정합니까?
주요 결과 측정
결과 측정 |
측정값 설명 |
기간 |
---|---|---|
Biochemical parameters (GSH and GSSG levels, GSH/GSSG ratio )
기간: Baseline and 1 month after treatment
|
The changes in levels of reduced and oxidized glutathione (GSH and GSSG) 1 month after periodontal treatment determined by ELISA.
The changes in levels of GSH were analyzed to determine diagnostic and prognostic potential as a biomarker of periodontitis and obesity.
|
Baseline and 1 month after treatment
|
2차 결과 측정
결과 측정 |
측정값 설명 |
기간 |
---|---|---|
프로빙 포켓 깊이
기간: 베이스라인 및 치료 1개월 후
|
치주 치료 후 프로빙 포켓 깊이의 변화. 질병의 중증도 및 임상 결과를 결정하기 위해 프로빙 포켓 깊이를 측정했습니다.
|
베이스라인 및 치료 1개월 후
|
포켓 깊이 및 임상 부착 수준 탐색
기간: 베이스라인 및 치료 1개월 후
|
치주치료 후 임상적 애착 정도의 변화.
프로빙 깊이와 치은 변연에서 백악질-에나멜 접합부까지의 거리는 임상 부착 수준을 측정하는 데 사용됩니다.
질병의 중증도 및 임상 결과를 결정하기 위해 임상 애착 수준을 측정했습니다.
|
베이스라인 및 치료 1개월 후
|
플라크 인덱스
기간: 베이스라인 및 치료 1개월 후
|
치주치료 후 치태지수 변화.
구강 위생 상태를 결정하고 분류하기 위해 플라크 지수를 기록했습니다.
|
베이스라인 및 치료 1개월 후
|
프로빙 시 출혈
기간: 베이스라인 및 치료 1개월 후
|
치주치료 후 탐침시 출혈의 변화.
치은 염증을 분류하고 평가하기 위해 프로빙 시 출혈을 기록했습니다.
|
베이스라인 및 치료 1개월 후
|
Gingival index
기간: Baseline and 1 month after treatment
|
he changes in gingival index after periodontal treatment.
Gingival index was recorded for classifying and evaluating (coronally) gingival inflammation.
|
Baseline and 1 month after treatment
|
공동 작업자 및 조사자
연구 기록 날짜
연구 주요 날짜
연구 시작
기본 완료 (실제)
연구 완료 (실제)
연구 등록 날짜
최초 제출
QC 기준을 충족하는 최초 제출
처음 게시됨 (추정)
연구 기록 업데이트
마지막 업데이트 게시됨 (추정)
QC 기준을 충족하는 마지막 업데이트 제출
마지막으로 확인됨
추가 정보
이 정보는 변경 없이 clinicaltrials.gov 웹사이트에서 직접 가져온 것입니다. 귀하의 연구 세부 정보를 변경, 제거 또는 업데이트하도록 요청하는 경우 register@clinicaltrials.gov. 문의하십시오. 변경 사항이 clinicaltrials.gov에 구현되는 즉시 저희 웹사이트에도 자동으로 업데이트됩니다. .
nonsurgical periodontal therapy에 대한 임상 시험
-
Alpha Tau Medical LTD.European Organisation for Research and Treatment of Cancer - EORTC아직 모집하지 않음
-
Alpha Tau Medical LTD.모병
-
Medical University of South CarolinaNational Institute on Deafness and Other Communication Disorders (NIDCD)모병
-
University of Alabama at Birmingham완전한
-
Abbott Medical Devices완전한