- ICH GCP
- US Clinical Trials Registry
- Klinisk forsøg NCT02498561
The Effects of Obesity on Glutathione Levels in Patients With Chronic Periodontitis Before and After Periodontal Therapy
Phase 1 Study of Periodontal Disease and Obese Individuals That Investigate of Local and Systemic Antioxidant Levels of Before and After Periodontal Treatment
Studieoversigt
Status
Betingelser
Intervention / Behandling
Detaljeret beskrivelse
The purpose of this study was to investigate the effects of obesity on glutathione levels in the plasma, GCF and saliva of patients with chronic periodontitis and to evaluate changes after nonsurgical periodontal therapy.
Diagnosed as obese (n=30) and normal-weight (n=30) individuals were categorized; chronic periodontitis (CP) and periodontally healthy controls (PH). Gingival crevicular fluid (GCF), plasma, saliva samples and clinical measurements were obtained at baseline and a month after nonsurgical periodontal therapy.
Unstimulated salivary samples were collected using standard techniques. About 2 mL whole saliva was collected in disposable tubes and centrifuged immediately to remove cell debris (10,000 g x 10 minutes). The supernatants (50µL each) were stored at -40C until analyzed. GCF samples were collected from a mesio-buccal and disto-palatal site on each tooth. In the CP group, the samples were obtained from patients at areas with ≥5 mm CAL, ≥6 mm PD and ≥30% bone loss. In gingivitis group, GCF samples were obtained from teeth with BOP and without CAL. In the healthy group, GCF samples were collected from teeth exhibiting PD<3 mm without CAL and BOP. The area was isolated with cotton rolls, saliva contamination elimination was ensured, and it was slightly air dried. GCF was sampled with paper strips. Paper strips were placed into the crevice until mild resistance was felt (intracrevicular method) and left in the position for 30 seconds. Strips contaminated with blood or saliva were discarded. Each sampled strip was placed into a 400µl eppendorf centrifuge tube and stored at -40C until analyzed.
Five milliliters of venous blood was taken from antecubital vein by using a standard venipuncture method. Obtained blood sample was collected in vacutainer tubes and anti-coagulated with EDTA. The blood samples were then stored at -40C until required for use in ELISAs.
Power analysis indicated that 12 individuals for each group would be sufficient to achieve 80% power to detect a difference of 0.05 between the alternative and the null hypotheses.
The Shapiro Wilk test was used to investigate whether or not the data were normally distributed. Continuous variables with unequal variances were compared by means of Welch and Tamhane's T2 post-hoc test for BMI, PD, CAL and the levels of glutathione. The comparison of the age, GI, PI and BOP was analyzed using the Kruskal-Wallis non-parametric test followed by post hoc group comparisons with the Bonferroni-adjusted Mann-Whitney U test. Paired Student's t-test or Wilcoxon rank-sum test was used to compare the measurements at two points (baseline and after SRP). The Spearman's rank correlation test was also used to detect the relationship between biochemical and clinical findings.
Undersøgelsestype
Tilmelding (Faktiske)
Fase
- Fase 1
Deltagelseskriterier
Berettigelseskriterier
Aldre berettiget til at studere
Tager imod sunde frivillige
Køn, der er berettiget til at studere
Beskrivelse
Inclusion Criteria:
- never-smokers
- no history of systemic disease
- no patients had been under periodontal therapy and medicine for at least 6 months before the study
- no pregnancy or lactation
- no alcohol or antioxidant vitamin consumption.
Exclusion Criteria:
-
Studieplan
Hvordan er undersøgelsen tilrettelagt?
Design detaljer
- Primært formål: Diagnostisk
- Tildeling: Randomiseret
- Interventionel model: Parallel tildeling
- Maskning: Firedobbelt
Våben og indgreb
Deltagergruppe / Arm |
Intervention / Behandling |
---|---|
Aktiv komparator: obese-chronic periodontitis patients
GCF, plasma and GCF samples were taken before and after nonsurgical periodontal therapy
|
Scaling and root planning under local anaesthesia, in a total of four clinical visits Oral hygiene instructions including the modified Bass technique, regular toothpaste, and an appropriate interdental cleaning device with dental floss and interdental brush.
Andre navne:
|
Placebo komparator: obese-periodontally healthy controls
GCF, plasma and GCF samples were taken at baseline after oral hygiene instructions
|
Oral hygiene instructions including the modified Bass technique, regular toothpaste, and an appropriate interdental cleaning device with dental floss and interdental brush
Andre navne:
|
Aktiv komparator: normal weight-CP patients
GCF, plasma and GCF samples were taken before and after nonsurgical periodontal therapy
|
Scaling and root planning under local anaesthesia, in a total of four clinical visits Oral hygiene instructions including the modified Bass technique, regular toothpaste, and an appropriate interdental cleaning device with dental floss and interdental brush.
Andre navne:
|
Placebo komparator: normal weight-PH controls
GCF, plasma and GCF samples were taken at baseline after oral hygiene instructions
|
Oral hygiene instructions including the modified Bass technique, regular toothpaste, and an appropriate interdental cleaning device with dental floss and interdental brush
Andre navne:
|
Hvad måler undersøgelsen?
Primære resultatmål
Resultatmål |
Foranstaltningsbeskrivelse |
Tidsramme |
---|---|---|
Biochemical parameters (GSH and GSSG levels, GSH/GSSG ratio )
Tidsramme: Baseline and 1 month after treatment
|
The changes in levels of reduced and oxidized glutathione (GSH and GSSG) 1 month after periodontal treatment determined by ELISA.
The changes in levels of GSH were analyzed to determine diagnostic and prognostic potential as a biomarker of periodontitis and obesity.
|
Baseline and 1 month after treatment
|
Sekundære resultatmål
Resultatmål |
Foranstaltningsbeskrivelse |
Tidsramme |
---|---|---|
Sonderende lommedybde
Tidsramme: Baseline og 1 måned efter behandling
|
Ændringerne i sonderingslommedybden efter parodontalbehandling. Sondelommedybden blev målt til bestemmelse af sygdommens sværhedsgrad og klinikudfald.
|
Baseline og 1 måned efter behandling
|
Undersøgelse af lommedybde og klinisk fastgørelsesniveau
Tidsramme: Baseline og 1 måned efter behandling
|
Ændringerne i klinisk tilknytningsniveau efter parodontal behandling.
Sondedybden og afstanden fra tandkødsranden til cemento-emaljeforbindelsen bruges til måling af klinisk vedhæftningsniveau.
Klinisk tilknytningsniveau blev målt for at bestemme sværhedsgraden af sygdommen og klinikresultatet.
|
Baseline og 1 måned efter behandling
|
Plaque indeks
Tidsramme: Baseline og 1 måned efter behandling
|
Ændringerne i plakindeks efter parodontalbehandling.
Plakindeks blev registreret for at bestemme og klassificere mundhygiejnestatus.
|
Baseline og 1 måned efter behandling
|
Blødning ved sondering
Tidsramme: Baseline og 1 måned efter behandling
|
Ændringerne i blødning ved sondering efter parodontalbehandling.
Blødning ved sondering blev registreret for at klassificere og evaluere (apikalt) gingival inflammation.
|
Baseline og 1 måned efter behandling
|
Gingival index
Tidsramme: Baseline and 1 month after treatment
|
he changes in gingival index after periodontal treatment.
Gingival index was recorded for classifying and evaluating (coronally) gingival inflammation.
|
Baseline and 1 month after treatment
|
Samarbejdspartnere og efterforskere
Sponsor
Samarbejdspartnere
Datoer for undersøgelser
Studer store datoer
Studiestart
Primær færdiggørelse (Faktiske)
Studieafslutning (Faktiske)
Datoer for studieregistrering
Først indsendt
Først indsendt, der opfyldte QC-kriterier
Først opslået (Skøn)
Opdateringer af undersøgelsesjournaler
Sidste opdatering sendt (Skøn)
Sidste opdatering indsendt, der opfyldte kvalitetskontrolkriterier
Sidst verificeret
Mere information
Begreber relateret til denne undersøgelse
Yderligere relevante MeSH-vilkår
Andre undersøgelses-id-numre
- 2013-62550515-02
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