Iron Absorption and Utilization in Adolescents Infected With Malaria Parasites, Hookworms or Schistosoma

Iron Absorption and Utilization in Adolescents Infected With Malaria Parasites, Hookworms or Schistosoma.

The aim of this study is to investigate the change in iron metabolism in relation to malaria and helminth infections using a stable isotope technique.

Study Overview

• Status: Completed
• Conditions: Malaria, Falciparum; Hookworm Infections; Schistosoma Haematobium
• Intervention / Treatment: Dietary supplement: Fe-57 orally, Fe-58 intravenously

Detailed Description

The aim of the study is to determine how iron metabolism in subjects with infectious diseases (malaria, hookworm or S. haematobium) differs while infected and after treatment, i.e. the same individuals will be restudied while free of infection.

• Study Type: Interventional
• Enrollment (Actual): 43
• Phase: Not Applicable

Contacts and Locations

Study Locations

• Côte D'Ivoire
  • Taabo, Côte D'Ivoire
    • Taabo Cité Hospital

Participation Criteria

Eligibility Criteria

• Ages Eligible for Study: 12 years to 16 years (CHILD)
• Accepts Healthy Volunteers: No
• Genders Eligible for Study: All

Description

Inclusion Criteria:

• Children aged 12 to 16 years
• Body weight > 30 kg
• no chronic medical illnesses
• no intake of vitamin/mineral supplements 2 weeks before the study and until the last blood drawing
• For each of the 4 arms the respective disease needs to be present (symptomatic malaria, asymptomatic malaria, hookworm infection, S. haematobium infection) without any other concurrent infection

Exclusion Criteria:

-

Study Plan

How is the study designed?

Design Details

• Primary Purpose: BASIC_SCIENCE
• Allocation: NON_RANDOMIZED
• Interventional Model: PARALLEL
• Masking: NONE

Number of Arms

4

Arms and Interventions

Participant Group / Arm	Intervention / Treatment
OTHER: Symptomatic malaria infection	Dietary supplement: Fe-57 orally, Fe-58 intravenously; Subjects will receive a test drink containing 3 mg Fe-57 in the form of labelled FeSO4 followed by 1 ml of an aqueous solution containing 50 μg Fe-58 as iron citrate.
OTHER: Asymptomatic malaria infection	Dietary supplement: Fe-57 orally, Fe-58 intravenously; Subjects will receive a test drink containing 3 mg Fe-57 in the form of labelled FeSO4 followed by 1 ml of an aqueous solution containing 50 μg Fe-58 as iron citrate.
OTHER: Hookworm infection	Dietary supplement: Fe-57 orally, Fe-58 intravenously; Subjects will receive a test drink containing 3 mg Fe-57 in the form of labelled FeSO4 followed by 1 ml of an aqueous solution containing 50 μg Fe-58 as iron citrate.
OTHER: Schistosoma haematobium infection	Dietary supplement: Fe-57 orally, Fe-58 intravenously; Subjects will receive a test drink containing 3 mg Fe-57 in the form of labelled FeSO4 followed by 1 ml of an aqueous solution containing 50 μg Fe-58 as iron citrate.

What is the study measuring?

Primary Outcome Measures

Outcome Measure	Time Frame
Isotope absorption into blood stream	Day 15
Isotope absorption into blood stream	Day 43

Secondary Outcome Measures

Outcome Measure	Time Frame
Isotope incorporation into erythrocytes	Day 15
Biochemical parameters of iron status and infection	Day 1
Biochemical parameters of iron status and infection	Day 15
Biochemical parameters of iron status and infection	Day 29
Biochemical parameters of iron status and infection	Day 43
Isotope incorporation into erythrocytes	Day 43

Collaborators and Investigators

• Sponsor: Swiss Federal Institute of Technology
• Investigators: Principal Investigator: Rita Wegmüller, Dr., Swiss Federal Institute of Technology (ETH) Zurich

Publications and helpful links

General Publications

• Rohner F, Zimmermann MB, Amon RJ, Vounatsou P, Tschannen AB, N'goran EK, Nindjin C, Cacou MC, Te-Bonle MD, Aka H, Sess DE, Utzinger J, Hurrell RF. In a randomized controlled trial of iron fortification, anthelmintic treatment, and intermittent preventive treatment of malaria for anemia control in Ivorian children, only anthelmintic treatment shows modest benefit. J Nutr. 2010 Mar;140(3):635-41. doi: 10.3945/jn.109.114256. Epub 2010 Jan 27.
• Wegmuller R, Camara F, Zimmermann MB, Adou P, Hurrell RF. Salt dual-fortified with iodine and micronized ground ferric pyrophosphate affects iron status but not hemoglobin in children in Cote d'Ivoire. J Nutr. 2006 Jul;136(7):1814-20. doi: 10.1093/jn/136.7.1814.
• Kastenmayer P, Davidsson L, Galan P, Cherouvrier F, Hercberg S, Hurrell RF. A double stable isotope technique for measuring iron absorption in infants. Br J Nutr. 1994 Mar;71(3):411-24. doi: 10.1079/bjn19940148.
• Walczyk T, Davidsson L, Zavaleta N, Hurrell RF. Stable isotope labels as a tool to determine the iron absorption by Peruvian school children from a breakfast meal. Fresenius Journal of Analytical Chemistry 359: 445-449, 1997.
• Glinz D, Hurrell RF, Righetti AA, Zeder C, Adiossan LG, Tjalsma H, Utzinger J, Zimmermann MB, N'Goran EK, Wegmuller R. In Ivorian school-age children, infection with hookworm does not reduce dietary iron absorption or systemic iron utilization, whereas afebrile Plasmodium falciparum infection reduces iron absorption by half. Am J Clin Nutr. 2015 Mar;101(3):462-70. doi: 10.3945/ajcn.114.090175. Epub 2015 Feb 4.

Study record dates

Study Major Dates

• Study Start: April 1, 2010
• Primary Completion (ACTUAL): September 1, 2013
• Study Completion (ACTUAL): September 1, 2013

Study Registration Dates

• First Submitted: July 14, 2010
• First Submitted That Met QC Criteria: July 15, 2010
• First Posted (ESTIMATE): July 16, 2010

Study Record Updates

• Last Update Posted (ESTIMATE): October 14, 2013
• Last Update Submitted That Met QC Criteria: October 11, 2013
• Last Verified: October 1, 2013

More Information

Terms related to this study

• Keywords: malaria; iron absorption; helminth; iron utilization; Ivory Coast; Acute symptomatic uncomplicated falciparum malaria; Asymptomatic falciparum malaria; Moderate to heavy hookworm infection; Heavy Schistosoma haematobium infection
• Additional Relevant MeSH Terms: Vector Borne Diseases; Parasitic Diseases; Protozoan Infections; Secernentea Infections; Nematode Infections; Helminthiasis; Strongylida Infections; Infections; Malaria; Malaria, Falciparum; Hookworm Infections; Ancylostomiasis
• Other Study ID Numbers: EK-2009-N-19