Matrilysin Expression in Different Stages of Colorectal Tumors (MMP7)

Matrix metalloproteinases (MMPs) have been shown to be involved in cancer biology. Significant expression of MMP-7 (matrilysin) in colorectal cancer is mainly associated with metastatic disease even though it is expressed in most tumor states. Our purpose is to analyse MMP-7 in bowel and lymph nodes of different tumor stages and to evaluate its expression as a potential biomarker of cancer disease in patients surgically treated for benign and malignant colorectal tumors. Tumoral tissue, lymph nodes and serum samples from recruited Patients plus serum samples from healthy volunteers are analysed for matrilysin expression by histology, immunohistochemistry, ELISA and Western blotting.

If Matrilysin increases with increasing dysplasia and cancer disease stage in tumor tissue as well as in the regional lymph nodes it might be used as a complement in investigating suspected locally advanced cancer.

Study Overview

• Status: Completed
• Conditions: Colorectal Cancer Stage II; Colorectal Cancer Stage III; Colorectal Cancer Stage IV; Colorectal Cancer Stage 0; Colorectal Cancer Stage I
• Intervention / Treatment: Procedure: colonic or colorectal resection

• Study Type: Observational
• Enrollment (Actual): 38

Contacts and Locations

Study Locations

• Italy
  • Rome, Italy, 00161
    • Departement of Surgery Pietro Valdoni

Participation Criteria

Eligibility Criteria

• Ages Eligible for Study: 40 years to 83 years (Adult, Older Adult)
• Accepts Healthy Volunteers: No
• Genders Eligible for Study: All

• Sampling Method: Non-Probability Sample

Study Population

patients affected by colorectal neoplasm admitted to our department of surgery

Description

Inclusion Criteria:

• benign colonic neoplasm not suitable for endoscopic treatment,
• malignant colonic neoplasm with indication to primary surgical treatment

Exclusion Criteria:

• neo-adjuvant radiotherapy,
• chemo-radiotherapy,
• language problems and withdrawal of consent

Study Plan

How is the study designed?

Design Details

• Observational Models: Case-Control
• Time Perspectives: Prospective

Number of groups / cohorts

4

Cohorts and Interventions

Group / Cohort	Intervention / Treatment
healthy volunteers; healthy subjects not affected by benign or malignant colonic disease	Procedure: colonic or colorectal resection; standard laparotomic resection of colon-rectum including right hemicolectomy, left hemicolectomy, sigmoidectomy, anterior resection
benign colonic tumor patients; patients affected by benign colonic tumor such as adenoma	Procedure: colonic or colorectal resection; standard laparotomic resection of colon-rectum including right hemicolectomy, left hemicolectomy, sigmoidectomy, anterior resection
cancer patient I-II stage; Patients affected by colonic cancer in I-II stage	Procedure: colonic or colorectal resection; standard laparotomic resection of colon-rectum including right hemicolectomy, left hemicolectomy, sigmoidectomy, anterior resection
cancer patients III-IV stage; Patients affected by colonic cancer in III-IV stage	Procedure: colonic or colorectal resection; standard laparotomic resection of colon-rectum including right hemicolectomy, left hemicolectomy, sigmoidectomy, anterior resection

Collaborators and Investigators

• Sponsor: University of Roma La Sapienza
• Investigators: Principal Investigator: Andrea Polistena, MD, Department of Surgery Pietro Valdoni, University La Sapienza Rome

Publications and helpful links

General Publications

• Mori M, Barnard GF, Mimori K, Ueo H, Akiyoshi T, Sugimachi K. Overexpression of matrix metalloproteinase-7 mRNA in human colon carcinomas. Cancer. 1995 Mar 15;75(6 Suppl):1516-9. doi: 10.1002/1097-0142(19950315)75:6+3.0.co;2-7.

Study record dates

Study Major Dates

• Study Start: September 1, 2005
• Primary Completion (Actual): September 1, 2008
• Study Completion (Actual): February 1, 2012

Study Registration Dates

• First Submitted: March 31, 2012
• First Submitted That Met QC Criteria: April 3, 2012
• First Posted (Estimate): April 4, 2012

Study Record Updates

• Last Update Posted (Estimate): April 4, 2012
• Last Update Submitted That Met QC Criteria: April 3, 2012
• Last Verified: March 1, 2012

More Information

Terms related to this study

• Keywords: colorectal cancer; adenoma; matrilysin; MMP-7; lymph nodes
• Additional Relevant MeSH Terms: Digestive System Diseases; Neoplasms; Neoplasms by Site; Gastrointestinal Neoplasms; Digestive System Neoplasms; Gastrointestinal Diseases; Colonic Diseases; Intestinal Diseases; Intestinal Neoplasms; Rectal Diseases; Colorectal Neoplasms
• Other Study ID Numbers: andreapolistena1