Cervical Mucus - the Biochemical and Molecular Properties in Fertile and Subfertile Women (C-MIS Study) (C-MIS)

Cervical Mucus - the Biochemical and Molecular Properties in Fertile and Subfertile Women

Infertility affects approximately one in six couples1. In approximately one third of cases, there is no cause found as to why a couple are unable to conceive2. In order for natural pregnancy to occur, sperm must pass through the cervix (neck of the womb) and swim to the woman's Fallopian tubes so that fertilisation of one of the woman's eggs can occur. It is known that mid-cycle mucus at the cervix is essential for sperm to gain access to the uterus and tubes.

There is evidence that the composition of this mucus in women may affect fertility but this area has not been studied well in recent years, partly because fertility treatments such as IVF bypass the cervix.

Research in sheep and cows in UCD has shown interesting differences in the cervical mucus of fertile and infertile animals. It appears that the mucus not only helps sperm to get to the uterus but may also help the sperm to mature and be ready to fertilise an egg. This mucus may also help prevent bacteria and infection reaching the womb.

In conjunction with colleagues in UCD, under the leadership of Professor S Carrington, the investigators would like to investigate some of the properties of human cervical mucus.The investigators propose to do this by taking samples of cervical mucus around the time of ovulation and also approximately one week later in fertile women and sub-fertile women. Then to monitor ovulation with an ultrasound scan (follicle tracking) of the woman's ovaries and urinary ovulation kits and take blood for oestrogen, progesterone, LH and FSH levels.

The investigators wish to determine if there are differences between the mucus of these women and how it may impact on sperm function and on fertility.

Study Overview

• Status: Unknown
• Conditions: Infertility

Detailed Description

This study will investigate functional, biochemical and molecular properties of cervical mucus at various times of the menstrual cycle in fertile and sub-fertile women. The investigators would aim to also investigate the interaction of cervical mucus with sperm and capacitation and to investigate the impact cervical surgery and differing diagnoses of sub-fertility have on cervical mucus.

Study aims:

1. To determine the functional, biochemical and molecular properties of cervical mucus through out the menstrual cycle in fertile women.
2. To determine if there is a difference in these properties of cervical mucus in fertile and sub-fertile women.
3. To determine whether these properties are influenced by the sub-fertility diagnostic group eg unexplained, endometriosis, tubal factor, sperm factor.
4. To examine the interaction between cervical mucus and sperm in women with normal fertility and sub-fertility.
5. To examine the impact of cervical surgery on cervical mucus functional, biochemical and molecular properties and sperm interaction.

• Study Type: Observational
• Enrollment (Anticipated): 90

Contacts and Locations

• Study Contact: Name: fiona m martyn, MB, MRCOG; Phone Number: 4058 00353-1-6635000; Email: f_martyn@yahoo.com
• Study Contact Backup: Name: mary wingfield, MD, FRCOG; Phone Number: 4003 00353-1-6635000; Email: mbwingfield@gmail.com

Study Locations

• Ireland
  • Dublin 2
    • Holles streetdublin 2, ireland, Dublin 2, Ireland
      • Recruiting
      • Merrion fertility clinic/national maternity hospital
      • Contact: fiona m martyn, MB,MRCOG; Phone Number: 4058 00353-1-6635000; Email: f_martyn@yahoo.com
      • Sub-Investigator: fiona m martyn, MB,MRCOG

Participation Criteria

Eligibility Criteria

• Ages Eligible for Study: 18 years to 42 years (Adult)
• Accepts Healthy Volunteers: Yes
• Genders Eligible for Study: Female

• Sampling Method: Non-Probability Sample

Study Population

gyanecological outpatient department

Description

Inclusion Criteria:

1. Fertile women

   1. Age 18-42 years
   2. Regular ovulatory menstrual cycle
   3. Previous spontaneous pregnancy to term
   4. No history of subfertility
   5. No underlying medical condition or immunosuppression
   6. No history of hormonal medication or pregnancy in past two months
   7. Capable of giving informed consent to participate in study
2. Non fertile women a.Age 18-42 years b.Regular ovulatory menstrual cycle c.Difficulty conceiving for greater than 12 months e.No underlying medical condition or immunosuppression f.No history of hormonal medication in past two months g.Capable of giving informed consent to participate in study

Exclusion Criteria:

1. hormonal medication
2. pregnancy
3. not capable of giving consent
4. age <18 years or > 42 years
5. underlying medical condition

Study Plan

How is the study designed?

What is the study measuring?

Primary Outcome Measures

Outcome Measure	Time Frame
The ability of sperm to swim through cervical mucus	2 years

Collaborators and Investigators

• Sponsor: Merrion Fertility Clinic
• Collaborators: University College Dublin
• Investigators: Principal Investigator: mary wingfield, MD, FRCOG, Merrion fertility clinic/national maternity hospital

Study record dates

Study Major Dates

• Study Start: August 1, 2012
• Primary Completion (Anticipated): August 1, 2014
• Study Completion (Anticipated): August 1, 2014

Study Registration Dates

• First Submitted: August 31, 2012
• First Submitted That Met QC Criteria: September 4, 2012
• First Posted (Estimate): September 5, 2012

Study Record Updates

• Last Update Posted (Estimate): September 6, 2013
• Last Update Submitted That Met QC Criteria: September 5, 2013
• Last Verified: September 1, 2013

More Information

Terms related to this study

• Keywords: infertility; interaction; cervical; mucus; sperm
• Additional Relevant MeSH Terms: Infertility
• Other Study ID Numbers: MFC 1