Bacterial Arrangement on the Teeth
Bacterial Arrangement in Supragingival Biofilms
Background:
- Dental plaque is a coating of bacteria on the teeth. It contributes to poor dental health and diseases such as gingivitis, cavities and periodontal disease. Researchers who study plaque know that many different types of oral bacteria stick to each other in test-tube experiments. However, it is not clear if these interactions occur in natural dental plaque. By studying how bacteria interact, researchers can better understand how bacteria come together and grow to form plaque.
Objectives:
- To look at how bacteria interact with one another to form dental plaque.
Eligibility:
- Healthy nonsmoking volunteers at least 18 years of age.
Design:
- Participants will be screened with a medical history and dental exam. They will also have dental impressions taken for a stent (a device similar to Invisalign(SqrRoot) braces).
- Participants will wear the stent for up to 8 hours a day on selected study visit days. It will contain enamel chips made from sterilized human teeth. The mouth bacteria will stick to the chips and grow. Chips will be taken out of the stent twice during these study visit days.
- Participants will have other study visits to provide saliva and plaque samples. They will also have gum swabs to collect bacteria.
- Some participants may have a second set of study visits. At these visits, they will chew gum to increase their saliva production. Afterwards, they will wear the stent with the enamel chips for 4 hours. Samples from the stent will be taken once on these study visit days.
Study Overview
Status
Detailed Description
Dental caries and periodontal disease are closely associated with the oral biofilm known as dental plaque. Much is known about the makeup of this biofilm, especially at its early stage of development (i.e. up to 12 hrs following tooth cleaning). The primary initial colonizers are streptococci, and actinomyces. These bacteria coaggregate frequently with one another by a mechanism that involves binding of protein adhesins on the surface of one cell to complementary cell surface receptor polysaccharides (RPS) on streptococci.
Coaggregation profiles are known for a wide range of oral actinomyces and streptococci. However, despite the potential significance of these interactions in biofilm formation, little is known about their occurrence within oral biofilms in situ.
Objective
The objective of this study is to determine and compare the influence of coaggregation on the spatial organization of bacteria in supragingival dental plaque formed under conditions of normal salivary flow (Arm 1 of the protocol) or increased salivary flow (Arm 2 of the protocol).
Study Population
The study population will consist of up to twenty-five (25) healthy adult subjects 18 years of age and older. All subjects will participate in Arm 1 of the protocol. In addition, at least three volunteers from Arm 1 will participate in Arm 2 of the protocol.
Study Design
This will be an observational, longitudinal protocol to determine the spatial organization of bacteria found within dental plaque of normal healthy subjects. Enrolled subjects will have a standard oral examination (including oral cancer screening) performed in the NIDCR clinic by protocol-listed dental clinicians. A mandibular stent will be custom fabricated based on impressions taken during the exam. Enamel chips cut from previously extracted third molars will be sterilized by ethylene oxide then inserted into the stent on each side over the molar area. We currently have enough extracted third molars in storage to accommodate tooth chips for the entire protocol if four subjects complete the study as projected. If additional chips are needed, the NIDCR is currently in the process of preparing a protocol that will provide extracted teeth, if needed, for this and other protocols. In Arm 1 of the study, a custom-fitted stent will be worn in the mouth of each subject, and chips removed for analysis after 4 or 8 hours of wearing. The bacterial biofilm on the chips will be stained with fluorescent antibodies against specific microbial adhesins or receptors, after which the spatial organization of labeled cells within the biofilm will be documented by laser confocal microscopy. Chips may be used for bacterial isolation and subjects will be asked to provide saliva samples, and site-specific plaque samples (curette scrapings, gum swabs) for molecular taxonomic analysis. Decisions on which subjects will have these samples taken will be based on the reactivity of available antibodies with bacterial communities. Subjects from Arm 1 of the study who are chosen to participate in Arm 2, will chew a gum base for 10 minutes to increase salivary flow while wearing their stent. The stent from these individuals will be removed after 4 hours of additional wear and analyzed as above. Prior to participation in Arm 2 of the protocol, subjects will be advised that the experiments are supported by a CRADA agreement. Through this agreement, only coded research information will be exchanged. The samples will remain with the NIH research team. Individuals may participate in the first arm only, or in both arms of the study, as indicated in their consent document.
Outcome Measures
Outcome measures for this protocol will be the qualitative and quantitative description of the oral bacterial community during development of initial plaque.
Study Type
Enrollment (Actual)
Contacts and Locations
Study Locations
-
-
Maryland
-
Bethesda, Maryland, United States, 20892
- National Institutes of Health Clinical Center
-
-
Participation Criteria
Eligibility Criteria
Ages Eligible for Study
Accepts Healthy Volunteers
Sampling Method
Study Population
Description
- INCLUSION CRITERIA:
- Healthy adult subjects (as determined from self-reported medical history, evaluation by study dentist and nursing assessment, per policy).
- 18 years of age or older
Good oral health as determined by screening oral examination with:
- no history of oral cancer
- no active periodontal disease.
EXCLUSION CRITERIA:
- History of any medical conditions affecting salivary flow, such as Sj(SqrRoot)(Delta)gren s syndrome
- History of any immunosuppressant therapy, such as glucocorticoids (within the last year), auto-immune or immune diseases such as ulcerative colitis or systemic lupus erythematosis, or current steroid therapy, other than topical, within the last 30 days
- History of cardiac, kidney, liver and lung disorders
- Use of tobacco within the last year
- Use of antibiotics within the preceding four months
- Use of medications thought to affect salivary flow such as head/neck radiation therapy, diuretics, or nitrates. Medications will be checked against product information for effect and reviewed by the protocol dentist.
- Clinically visible active dental caries.
- Active periodontal disease as defined as no more than 4 sites in the entire mouth with greater than or equal to 5mm probing depths.
- History of severe allergic reactions (Anaphylaxis) to several allergins and specific allergies to or other substances found in the stent or impression material (ie: Plasticized Methacrylate Polymer, methyl methacrylate monomer, alkyl dimethacrylate, benzophenone, Toluidine and Hydroquinone), waxes containing Pine Rosin, and to the list of ingredients for the impression material.
Study Plan
How is the study designed?
Design Details
- Observational Models: Case-Only
- Time Perspectives: Other
Cohorts and Interventions
Group / Cohort |
|---|
|
1
Healthy participants
|
What is the study measuring?
Primary Outcome Measures
Outcome Measure |
Measure Description |
Time Frame |
|---|---|---|
|
qualitatively analyze image data to determine the types of bacteria that directly interact with one another on the chip surface to understand community assembly during plaque accumulation
Time Frame: within 23 study visits
|
Bacteria will be isolated from chips and plaque samples.
Molecular descriptions of total flora (from chips, plaque samples and gum swabs) will provide a list of bacteria present at the sampling time.
|
within 23 study visits
|
Collaborators and Investigators
Investigators
- Principal Investigator: Niki M Moutsopoulos, D.D.S., National Institute of Dental and Craniofacial Research (NIDCR)
Publications and helpful links
Helpful Links
Study record dates
Study Major Dates
Study Start (Actual)
Primary Completion (Actual)
Study Completion (Actual)
Study Registration Dates
First Submitted
First Submitted That Met QC Criteria
First Posted (Estimated)
Study Record Updates
Last Update Posted (Actual)
Last Update Submitted That Met QC Criteria
Last Verified
More Information
Terms related to this study
Keywords
Additional Relevant MeSH Terms
Other Study ID Numbers
- 130014
- 13-D-0014
Plan for Individual participant data (IPD)
Plan to Share Individual Participant Data (IPD)?
IPD Plan Description
Drug and device information, study documents
Studies a U.S. FDA-regulated drug product
Studies a U.S. FDA-regulated device product
This information was retrieved directly from the website clinicaltrials.gov without any changes. If you have any requests to change, remove or update your study details, please contact register@clinicaltrials.gov. As soon as a change is implemented on clinicaltrials.gov, this will be updated automatically on our website as well.