Acute Effects of Natural and Interesterified Fats-margarine on Lipemic and Insulinemic Response

Acute Effects of Natural and Interesterified Fats-formulated Margarine on Lipemic and Insulinemic Response

Interesterified (IE) fat blends will respond differently in terms of postprandial lipemia and insulinemic responses compare to a trans free palm margarine (TFPM).

Study Overview

• Status: Completed
• Conditions: Healthy
• Intervention / Treatment: Other: Trans free palm margarine; Other: Interesterified Palm based margarine; Other: IE Soybean oil-based margarine

Detailed Description

The objective of this study was to investigate whether IE-fat blends behave differently on postprandial serum lipid, insulin, C-peptide and glucose concentration compare to TFPM. A randomised, double-blind crossover (3 × 3 arms) orthogonal Latin-square design was used and conducted on 10 males and 10 females adults. During postprandial challenge day, each subject received an a test muffin incorporated with test margarine. Blood samples were collected at half-hourly intervals until 4 hr by using cannulation technique.

• Study Type: Interventional
• Enrollment (Actual): 20
• Phase: Not Applicable

Contacts and Locations

Study Locations

• Malaysia
  • Selangor
    • Kajang, Selangor, Malaysia, 43000
      • MalaysiaPOB

Participation Criteria

Eligibility Criteria

• Ages Eligible for Study: 20 years to 50 years (Adult)
• Accepts Healthy Volunteers: Yes
• Genders Eligible for Study: All

Description

Inclusion Criteria:

• Body Mass Index (BMI) between 18.5 and 24.9
• <130mmHg for systolic pressure and <80 mmHg for diastolic pressure
• Blood glucose level in the range of 3.9 to 5.5 mmol/L
• Total cholesterol less than 5.17 mmol/L
• No use of tobacco and no consumption of alcohol.

Exclusion Criteria:

• Suffer from chronic disease
• Metabolic syndrome
• Consuming any medical prescriptions
• People with blood clotting problem
• Women who are pregnant
• Hypertensive person

Study Plan

How is the study designed?

Design Details

• Allocation: Randomized
• Interventional Model: Crossover Assignment
• Masking: Quadruple

Number of Arms

3

Arms and Interventions

Participant Group / Arm	Intervention / Treatment
Experimental: Trans free palm margarine; One high fat muffin will be serves together with a glass of low fat milk shake.	Other: Trans free palm margarine; A randomised, double-blind crossover (3 × 3 arms) orthogonal Latin-square design was used and conducted on 10 males and 10 females adults.; Other: Interesterified Palm based margarine; A randomised, double-blind crossover (3 × 3 arms) orthogonal Latin-square design was used and conducted on 10 males and 10 females adults.; Other: IE Soybean oil-based margarine; A randomised, double-blind crossover (3 × 3 arms) orthogonal Latin-square design was used and conducted on 10 males and 10 females adults.
Experimental: Interesterified palm based margarine; One high fat muffin will be serves together with a glass of low fat milk shake.	Other: Trans free palm margarine; A randomised, double-blind crossover (3 × 3 arms) orthogonal Latin-square design was used and conducted on 10 males and 10 females adults.; Other: Interesterified Palm based margarine; A randomised, double-blind crossover (3 × 3 arms) orthogonal Latin-square design was used and conducted on 10 males and 10 females adults.; Other: IE Soybean oil-based margarine; A randomised, double-blind crossover (3 × 3 arms) orthogonal Latin-square design was used and conducted on 10 males and 10 females adults.
Experimental: IE soybean oil-based margarine; One high fat muffin will be serves together with a glass of low fat milk shake.	Other: Trans free palm margarine; A randomised, double-blind crossover (3 × 3 arms) orthogonal Latin-square design was used and conducted on 10 males and 10 females adults.; Other: Interesterified Palm based margarine; A randomised, double-blind crossover (3 × 3 arms) orthogonal Latin-square design was used and conducted on 10 males and 10 females adults.; Other: IE Soybean oil-based margarine; A randomised, double-blind crossover (3 × 3 arms) orthogonal Latin-square design was used and conducted on 10 males and 10 females adults.

What is the study measuring?

Primary Outcome Measures

Outcome Measure	Time Frame
Change in serum triglycerides level of subjects as a measure of postprandial triglycerides response	Baseline, 4 hours

Secondary Outcome Measures

Outcome Measure	Time Frame
Change in the level of plasma insulin, c-peptide and serum glucose as a measure of postprandial insulinemic response	Baseline, 4 hours

Collaborators and Investigators

• Sponsor: Malaysia Palm Oil Board
• Collaborators: Universiti Putra Malaysia; International Medical University
• Investigators: Principal Investigator: Voon Phooi Tee, Ph.D, Malaysia Palm Oil Board

Study record dates

Study Major Dates

• Study Start: January 1, 2012
• Primary Completion (Actual): December 1, 2012
• Study Completion (Actual): December 1, 2012

Study Registration Dates

• First Submitted: June 25, 2014
• First Submitted That Met QC Criteria: July 16, 2014
• First Posted (Estimate): July 18, 2014

Study Record Updates

• Last Update Posted (Estimate): July 18, 2014
• Last Update Submitted That Met QC Criteria: July 16, 2014
• Last Verified: July 1, 2014

More Information

Terms related to this study

• Other Study ID Numbers: AH 00112