Effects of Co-ingesting a Low/High GI Meal With Different Fat Saturation on Postprandial Glucose, Insulin, and Triacylglycerol Responses in Healthy Participants

It is now recognized that diet plays a critical role in the etiology and management of the chronic diseases such as diabetes, obesity and cardiovascular disease. A high consumption of fat, especially saturated fat, is an established risk factor for Cardiovascular Disease.The glycaemic index (GI) is a measurement of the ability of different types of carbohydrate-based foods to raise blood glucose. The interest in low GI food as a weight management tool has been increasing. Different types of carbohydrates differ in their ability to increase postprandial blood glucose and insulin secretion, and may also modulate the effect of different fatty acids. The current study will compare the consumption of low and high GI carbohydrate with different fat saturation on postprandial glucose, insulin, and triacylglycerol responses in healthy participants.

The data generated will have direct applications in public health policy and clinical nutrition management of chronic disease. The outcomes of the study will significantly contribute to the long term national goals of Singapore to manage and to reduce the incidence of chronic disease associated with the metabolic syndrome.

Study Overview

• Status: Completed
• Conditions: Healthy
• Intervention / Treatment: Dietary supplement: low glycemic index rice, high glycemic index rice, butter, olive oil and grapeseed oil

Detailed Description

This study will be conducted using a randomized, crossover design with six dietary phases: a low GI meal or a high GI meal with saturated fat, monounsaturated fat or polyunsaturated fat. All participants will be randomly allocated to the six test meals, with the order balanced. Fifteen participants aged between 21 and 40 years will be recruited from the general public in Singapore. During the testing session, a maximum of eleven blood samples will be collected by fingerpricks and cannulation. Fingerpricks and cannulation may cause minor discomfort and there may be some slight bruising around the site. Fingerpricks and cannulation will be done by an experienced research nurse or a trained research officer to minimize participant discomfort. If bruising does occur, it should disappear within one day and it should not affect participants' regular daily activity. Participants will be limited to a maximum of two tests per week. In addition, blood collection will be carried out in a sterile designated area and thus the risk of infection will be very low. On rare occasions, participants may feel unwell during or after the testing session. The investigators have several metabolic suites with beds for these individuals to rest on. The research nurse and research officers will monitor the individuals' condition and they will be provided with a ride home if required.

All the study foods will be prepared in a hygienic manner in a purpose-built research kitchen maintained to the highest hygiene standards. The research staff has undergone basic training in food hygiene procedures. Hence, the risk of infective acute gastroenteritis will be minimal.

A number of dietary interventions have aimed to investigate the effect of dietary fat quality on glucose, insulin and lipidemia response. Up to now there have been few studies connect the glycaemic load and fat quality together. The investigators study's results will help public choose fat quality and carbohydrate quality in the diet. Participants will be provided with some snacks at the end of each testing session.

In addition, participants will be provided with their blood glucose, blood insulin, blood lipid profile, blood pressure, and body composition results, with a brief interpretation of these at the conclusion of the study.

• Study Type: Interventional
• Enrollment (Actual): 25
• Phase: Not Applicable

Contacts and Locations

Study Locations

• Singapore
  • Singapore, Singapore, 117599
    • Clinical Nutrition Research Centre

Participation Criteria

Eligibility Criteria

• Ages Eligible for Study: 21 years to 40 years (Adult)
• Accepts Healthy Volunteers: No
• Genders Eligible for Study: Male

Description

Inclusion Criteria:

• Healthy Chinese male subjects aged between 21 and 40 years
• Do not have major chronic disease such as heart disease, cancer or diabetes mellitus
• Do not have family history of diabetes
• Do not have intolerances or allergies to test products(wheat and milk based products)
• Not taking insulin or drugs known to affect glucose or triglyceride levels and body fat distribution
• Do not have major medical or surgical event requiring hospitalization within the preceding three months

Study Plan

How is the study designed?

Design Details

• Primary Purpose: Basic Science
• Allocation: Randomized
• Interventional Model: Crossover Assignment
• Masking: None (Open Label)

Number of Arms

6

Arms and Interventions

Participant Group / Arm	Intervention / Treatment
Experimental: low glycemic index rice with butter; 50 g available low glycemic index rice cooked with 48 g butter ( equal 40 g saturated fatty acid)	Dietary supplement: low glycemic index rice, high glycemic index rice, butter, olive oil and grapeseed oil
Experimental: low glycemic index rice with olive oil; 50 g available low glycemic index rice cooked with 44 g olive oil ( equal 40 g monounsaturated fatty acid)	Dietary supplement: low glycemic index rice, high glycemic index rice, butter, olive oil and grapeseed oil
Experimental: low glycemic index rice with grapeseed oil; 50 g available low glycemic index rice cooked with 40 g grapeseed oil ( equal 40 g polyunsaturated fatty acid)	Dietary supplement: low glycemic index rice, high glycemic index rice, butter, olive oil and grapeseed oil
Experimental: high glycemic index rice with butter; 50 g available high glycemic index rice cooked with 48 g butter ( equal 40 g saturated fatty acid)	Dietary supplement: low glycemic index rice, high glycemic index rice, butter, olive oil and grapeseed oil
Experimental: high glycemic index rice with olive oil; 50 g available high glycemic index rice cooked with 44 g olive oil ( equal 40 g monounsaturated fatty acid)	Dietary supplement: low glycemic index rice, high glycemic index rice, butter, olive oil and grapeseed oil
Experimental: high glycemic index rice with grapeseed oil; 50 g available high glycemic index rice cooked with 40 g grapeseed oil ( equal 40 g polyunsaturated fatty acid)	Dietary supplement: low glycemic index rice, high glycemic index rice, butter, olive oil and grapeseed oil

What is the study measuring?

Primary Outcome Measures

Outcome Measure	Measure Description	Time Frame
Glucose response of different treatments	The blood glucose will be measured by finger prick and venous blood collection at the same time points	4 hours postprandial
Insulin response of different treatments	Insulin concentration will be measured by COBAS Roche analyzer e311	4 hours postprandial
Triglycerides response of different treatments	Triglycerides will be measured by COBAS Roche analyzer e411	4 hours postprandial

Secondary Outcome Measures

Outcome Measure	Measure Description	Time Frame
Active Glucagon-like peptide 1 response of different treatments	Active Glucagon-like peptide 1 will be measured by Multiplex kits	4 hours postprandial
Gastric inhibitory polypeptide response of different treatments	Gastric inhibitory polypeptide will be measured by Multiplex kits	4 hours postprandial

Collaborators and Investigators

• Sponsor: Clinical Nutrition Research Centre, Singapore

Publications and helpful links

General Publications

• Sun L, Tan KWJ, Lim JZ, Magkos F, Henry CJ. Dietary fat and carbohydrate quality have independent effects on postprandial glucose and lipid responses. Eur J Nutr. 2018 Feb;57(1):243-250. doi: 10.1007/s00394-016-1313-y. Epub 2016 Oct 21.

Study record dates

Study Major Dates

• Study Start: November 1, 2014
• Primary Completion (Actual): April 1, 2016
• Study Completion (Actual): April 1, 2016

Study Registration Dates

• First Submitted: October 14, 2015
• First Submitted That Met QC Criteria: October 22, 2015
• First Posted (Estimate): October 23, 2015

Study Record Updates

• Last Update Posted (Estimate): August 2, 2016
• Last Update Submitted That Met QC Criteria: August 1, 2016
• Last Verified: August 1, 2016

More Information

Terms related to this study

• Keywords: Diabetes; Obesity; metabolic disorders
• Other Study ID Numbers: 2014/00719