Influence of Carbohydrate on Amino Acid Absorption From Dietary Protein (ICADP) (ICADP)

Influence of Carbohydrate on Amino Acid Absorption From Dietary Protein

10 young males will be recruited to participate in a randomized double blinded crossover study to investigate the influence of concomitant intake of two different types of carbohydrate with protein hydrolysate on the absorption kinetics of protein-derived amino acids and the impact on myofibrillar protein synthesis. During the trial days the subjects will perform a bout of whole body resistance exercise and ingest a supplement of meat hydrolysate labeled with D5-phenylalanine together with randomized and double blinded type of carbohydrate consisting of either glucose or cluster dextrin.

The primary outcome is the time to obtain peak concentration of D5-phenylalanine after intake of meat hydrolysate with either glucose or cluster dextrin.

Hypotheses:

I) The cluster dextrin will increase the absorption of the amino acids from the meat hydrolysate, and thereby result in a faster rise and a higher peak in plasma amino acid concentration in the postprandial period.

II) The meat hydrolysate will increase the protein synthesis rate to a higher extent when combined with cluster dextrin than with glucose.

Study Overview

• Status: Completed
• Conditions: Muscle Protein Synthesis; Amino Acid
• Intervention / Treatment: Dietary supplement: Meat Hydrolysate; Dietary supplement: Cluster Dextrin; Dietary supplement: Placebo

• Study Type: Interventional
• Enrollment (Actual): 10
• Phase: Not Applicable

Contacts and Locations

Study Locations

• Denmark
  • Copenhagen NV
    • Copenhagen, Copenhagen NV, Denmark, 2400
      • Bispebjerg Hospital

Participation Criteria

Eligibility Criteria

• Ages Eligible for Study: 14 years to 26 years (Adult)
• Accepts Healthy Volunteers: No
• Genders Eligible for Study: Male

Description

Inclusion Criteria:

• young healthy adults,
• organised sport/training 1-3 times per week

Exclusion Criteria:

• Subject younger than 18 years or above 30 years
• BMI > 30
• Chronic medical diseases: active cancer, renal diseases, metabolic diseases like diabetes mellitus.
• Physical inactivity. Subjects must participate in systematized weekly exercise 1-3 times per week.
• Participating in systematized exercise more than 3 times per week.
• Vegetarian.
• Participating in a special diet which deviates from the standard of young adults.

Study Plan

How is the study designed?

Design Details

• Primary Purpose: Basic Science
• Allocation: Randomized
• Interventional Model: Crossover Assignment
• Masking: Double

Number of Arms

2

Arms and Interventions

Participant Group / Arm	Intervention / Treatment
Experimental: Meat hydrolysate & Cluster Dextrin; Meat hydrolysate containing 25g of protein given together with 75 grams of Cluster Dextrin.	Dietary supplement: Meat Hydrolysate; 25 grams of meat hydrolysate to maximally stimulate protein synthesis.; Dietary supplement: Cluster Dextrin; 75 grams of Cluster Dextrin; Other Names: CAS no.: 9050-36-6; Glico Nutrition Co., Ltd.
Active Comparator: Meat hydrolysate & placebo; Meat hydrolysate containing 25g of protein given together with 75 grams of Glucose.	Dietary supplement: Meat Hydrolysate; 25 grams of meat hydrolysate to maximally stimulate protein synthesis.; Dietary supplement: Placebo; 75 grams of Glucose; Other Names: Glucose

What is the study measuring?

Primary Outcome Measures

Outcome Measure	Measure Description	Time Frame
Protein hydrolysate amino acid label time to peak appearance in plasma	Difference between the time to peak of protein-derived D5-phenylalanine amino acid appearance in the plasma	From hour 0 to hour 3

Secondary Outcome Measures

Outcome Measure	Measure Description	Time Frame
Myofibrillar protein fractional synthesis rate response to protein and carbohydrate intake after a whole body resistance exercise	Difference between myofibrillar protein fractional synthesis rate after meat hydrolysate with either 75 grams of Cluster Dextrin or Clucose.	from hour 0,5 to hour hour 1. From hour 1 to hour 3. From hour 0,5 to hour 3.
Protein hydrolysate amino acid label area under the concentration curve in plasma	Difference between the area under the concentration curve of protein-derived D5-phenylalanine amino acid appearance in the plasma	From hour 0 to hour 3
Insulin concentration	Difference between insulin concentrations after intake of the two carbohydrate types.	From hour 0 to hour 3

Collaborators and Investigators

• Sponsor: Bispebjerg Hospital
• Collaborators: University of Birmingham
• Investigators: Principal Investigator: Jacob L. Bülow, MD, Bispebjerg Hospital; Principal Investigator: Lars Holm, Prof., University of Birmingham; Principal Investigator: Michael Kjaer, MD, prof., Bispebjerg Hospital; Principal Investigator: Sofus Sølyst, stud.med., University of Copenhagen; Principal Investigator: Mikkel Jensen, Stud.scient., University of Copenhagen

Publications and helpful links

General Publications

• Nishimura Y, Jensen M, Bulow J, Thomsen TT, Arimitsu T, van Hall G, Fujita S, Holm L. Co-ingestion of cluster dextrin carbohydrate does not increase exogenous protein-derived amino acid release or myofibrillar protein synthesis following a whole-body resistance exercise in moderately trained younger males: a double-blinded randomized controlled crossover trial. Eur J Nutr. 2022 Aug;61(5):2475-2491. doi: 10.1007/s00394-021-02782-y. Epub 2022 Feb 19.

Study record dates

Study Major Dates

• Study Start (Actual): November 24, 2017
• Primary Completion (Actual): February 23, 2018
• Study Completion (Actual): February 23, 2018

Study Registration Dates

• First Submitted: October 3, 2017
• First Submitted That Met QC Criteria: October 3, 2017
• First Posted (Actual): October 6, 2017

Study Record Updates

• Last Update Posted (Actual): February 27, 2018
• Last Update Submitted That Met QC Criteria: February 26, 2018
• Last Verified: February 1, 2018

More Information

Terms related to this study

• Keywords: Exercise training; Carbohydrate; Protein supplement; Cluster Dextrin
• Other Study ID Numbers: H-17017363

Drug and device information, study documents

• Studies a U.S. FDA-regulated drug product: No
• Studies a U.S. FDA-regulated device product: No