- ICH GCP
- US Clinical Trials Registry
- Clinical Trial NCT04274582
The Influences of Chokeberry Extract Supplementation on Handball Players
The Influences of Chokeberry Extract Supplementation on Redox Status and Body Composition in Handball Players During Competition Phase
The study included 16 handball players, aged 16-24 years , of handball club "Novi Beograd". All study participants were apparently healthy, had no active sports injuries, did not use any medications, and were non-smokers. Standardized questionnaires conducted under the supervision of a trained nutritionist were used to collect general data, nutritional habits and use of dietary supplements. The athletes who used any dietary supplements at least a month before the study, were excluded. All participants (or their parents if they were under the age of 18) signed an informed consent document. The study was approved by the Ethical committee of The Military Medical Academy, Belgrade.
The study was conducted during regular competition season, and lasted for twelve weeks. All participants had the same training and nutritional regime, which excluded intake of berries. The players received 30 mL of liquid chokeberry extract, in the morning before training, once per day for 12 weeks. For the preparation of chokeberry extract (liquid form) was used fruit (berries) of Aronia melanocarpa Elliot, Rosaceae. The extract was donated by Pharmanova Belgrade, Serbia. Process of extraction is performed under specific conditions which are subject of technical patent (producer EUHEM), for the purpose of production of extract with high concentration of polyphenols. The design of the product included few demands: sufficient daily dose of polyphenols to be dietary supplement, small volume which can be consumed as shot and acceptable taste for most consumers. Compliance was monitored by the trainers.
Study Overview
Status
Conditions
Intervention / Treatment
Study Type
Enrollment (Actual)
Phase
- Not Applicable
Contacts and Locations
Study Locations
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Kragujevac, Serbia
- Faculty of Medical Science
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Participation Criteria
Eligibility Criteria
Ages Eligible for Study
Accepts Healthy Volunteers
Genders Eligible for Study
Description
Inclusion Criteria:
- healthy handball players of handball club "Novi Beograd" during competition phase.
Exclusion Criteria:
- active sports injuries,
- usage of any medications,
- smokers
Study Plan
How is the study designed?
Design Details
- Primary Purpose: Prevention
- Allocation: N/A
- Interventional Model: Single Group Assignment
- Masking: None (Open Label)
Arms and Interventions
Participant Group / Arm |
Intervention / Treatment |
---|---|
Experimental: Chokeberry extract consumption
The players received 30 mL of liquid chokeberry extract, in the morning before training, once per day for 12 weeks.
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The players received 30 mL of liquid chokeberry extract, in the morning before training, once per day for 12 weeks.
Other Names:
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What is the study measuring?
Primary Outcome Measures
Outcome Measure |
Measure Description |
Time Frame |
---|---|---|
Superoxide anion radical
Time Frame: three months
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The concentration of O2- was measured after the reaction of nitro blue tetrazolium in TRIS buffer with plasma at 530 nm.
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three months
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hydrogen peroxide
Time Frame: three months
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The determination of H2O2 concentration is based on oxidation of phenol red using hydrogen peroxide, in reaction catalyzed by enzyme peroxidase from horse radish (POD).
The level of H2O2 was measured at 610 nm.
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three months
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nitrites
Time Frame: three months
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Nitric oxide (•NO) decomposes rapidly to form stable metabolite nitrite/nitrate products.
The method for detection of the plasma nitrite levels is based on the Griess reaction.
Nitrites (NO2-) were determined as an index of NO production with Griess reagent (forms purple diazocomplex).
Nitrites were measured at 550 nm.
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three months
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index of lipid peroxidation
Time Frame: three months
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The degree of lipid peroxidation in the plasma was estimated by measuring the TBARS using 1% thiobarbituric acid in 0.05 NaOH, incubated with plasma at 100 C for 15 min, and measured at 530 nm.
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three months
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Secondary Outcome Measures
Outcome Measure |
Measure Description |
Time Frame |
---|---|---|
Superoxide dismutase
Time Frame: three months
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SOD activity was determined by the epinephrine method of Beutler. 100 μl lysate and 1 mL of carbonate buffer were mixed; then 100 μl of epinephrine was added.
Detection was performed at 470 nm.
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three months
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Reduced glutathione
Time Frame: three months
|
The level of GSH was determined based on GSH oxidation with 5.5- dithio-bis-6.2-nitrobenzoic
acid using the method reported by Beutler.
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three months
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Catalase
Time Frame: three months
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CAT activity was determined according to Aebi.
Lysates were diluted with distilled water (1:7 v/v) and treated with chloroform-ethanol (0.6:1 v/v) to remove haemoglobin; then 50 ll of CAT buffer, 100 μl of sample, and 1 mL of 10 mM H2O2 were added to the samples.
Detection was performed at 360 nm.
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three months
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Collaborators and Investigators
Sponsor
Study record dates
Study Major Dates
Study Start (Actual)
Primary Completion (Actual)
Study Completion (Actual)
Study Registration Dates
First Submitted
First Submitted That Met QC Criteria
First Posted (Actual)
Study Record Updates
Last Update Posted (Actual)
Last Update Submitted That Met QC Criteria
Last Verified
More Information
Terms related to this study
Other Study ID Numbers
- Alixir400protectSportsmen
Plan for Individual participant data (IPD)
Plan to Share Individual Participant Data (IPD)?
Drug and device information, study documents
Studies a U.S. FDA-regulated drug product
Studies a U.S. FDA-regulated device product
This information was retrieved directly from the website clinicaltrials.gov without any changes. If you have any requests to change, remove or update your study details, please contact register@clinicaltrials.gov. As soon as a change is implemented on clinicaltrials.gov, this will be updated automatically on our website as well.
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