Muscle Response to High Intensity Interval Resistance Training (HIIRT) (HIIRT)

Effects of Exercise Intensity and Rest Intervals on Intracellular Signals and Anabolic Response of Skeletal Muscle to Resistance Training

Resistance training (RT) is one of the most important stimuli for muscle hypertrophy and it plays an important role on weight loss and fatty acid oxidation increase. RT affects anabolic pathways but the differences among various training techniques has been till now were poorly investigated. The aim of this study was to compare the effect of two different intensity of training, high-intensity interval resistance training (HIIRT) and traditional resistance training (TRT), on muscle signalling pathway.

Study Overview

• Status: Completed
• Conditions: Exercise; Resistance Training
• Intervention / Treatment: Other: High Intensity Interval Resistance Training; Other: Traditional Resistance Training

Detailed Description

Nine young healthy subjects performed HIIRT and TRT protocol in two different moments and with different legs. HIIRT technique consisted of three sets of: 6 repetitions at 6RM (6 repetitions maximum) and then 20 seconds of rest and 2/3 repetitions (until exhaustion) repeated for 3 times with 2'30" rest between sets; while TRT consisted of 3 sets of 15 reps with 75 sec of rest between sets. Biopsies from the vastus lateralis were taken at baseline (pre), immediately (0h) at the end of training, 6 hours (6h) and 24 hours (24h) after training. Western blot and RT-PCR mRNA (Reverse transcriptase-polymerase chain reaction) analysis were performed to assess muscle signalling pathway activation

• Study Type: Interventional
• Enrollment (Actual): 9
• Phase: Not Applicable

Contacts and Locations

Study Locations

• Italy
  • Padova, Italy, 35131
    • Nutrition and Exercise Lab, DSB, University of Padova

Participation Criteria

Eligibility Criteria

• Ages Eligible for Study: 20 years to 30 years (ADULT)
• Accepts Healthy Volunteers: Yes
• Genders Eligible for Study: All

Description

Inclusion Criteria:

• moderate active
• experience with resistance training

Exclusion Criteria:

• history of recent myocardial infarction,
• severe cardiac arrhythmia,
• unstable angina,
• poorly controlled hypertension,
• poorly controlled diabetes mellitus,
• frequent or complex ventricular ectopy which might interfere with one's ability to adhere to exercise protocols

Study Plan

How is the study designed?

Design Details

• Primary Purpose: BASIC_SCIENCE
• Allocation: RANDOMIZED
• Interventional Model: CROSSOVER
• Masking: SINGLE

Number of Arms

2

Arms and Interventions

Participant Group / Arm	Intervention / Treatment
EXPERIMENTAL: High Intensity Interval Resistance Training (HIIRT); HIIRT technique consisted of three sets of: 6 repetitions at 80% 1RM (1 repetition maximum) and then 20 seconds of rest and 2/3 repetitions (until exhaustion) repeated for 3 times with 2'30" rest between sets; while TRT consisted of 3 sets of 15 reps with 75 sec of rest between sets.	Other: High Intensity Interval Resistance Training; Subjects trained one leg with HIIRT
ACTIVE_COMPARATOR: Traditional Resistance Training (TRT); TRT protocol performed 3 series of 15 repetitions at 60% 1RM with 75" of rest between sets.	Other: Traditional Resistance Training; Subjects trained the other leg with HIIRT

What is the study measuring?

Primary Outcome Measures

Outcome Measure	Measure Description	Time Frame
pAMPKThr172 in skeletal muscle tissue measured by immunoblot	measurement of pAMPKThr172 in skeletal muscle tissue taken by biopsy	24 hours
pERK 1/2Thr202/Tyr204 in skeletal muscle tissue measured by immunoblot	measurement of pERK 1/2Thr202/Tyr204 in skeletal muscle tissue taken by biopsy	24 hours
pS6Ser235/236 in skeletal muscle tissue measured by immunoblot	measurement of pS6Ser235/236 in skeletal muscle tissue taken by biopsy	24 hours
p4EBP1Thr37/46 in skeletal muscle tissue measured by immunoblot	measurement of p4EBP1Thr37/46 in skeletal muscle tissue taken by biopsy	24 hours
ACC (acetyl-coenzyme A carboxylase) phosphorylation in skeletal muscle tissue measured by immunoblot	ACC phosphorylation in skeletal muscle tissue taken by biopsy	24 hours
IGF-1 mRNA (messenger ribonucleic acid) in skeletal muscle tissue measured by Quantitative polymerase chain reaction (qPCR)	IGF-1 mRNA in skeletal muscle tissue taken by biopsy	24 hours
IGF-1ea (insulin like growth factor) mRNA in skeletal muscle tissue measured by Quantitative polymerase chain reaction (qPCR)	IGF-1ea mRNA in skeletal muscle tissue taken by biopsy	24 hoursn
MGF (mechanical growth factor) mRNA in skeletal muscle tissue measured by Quantitative polymerase chain reaction (qPCR)	MGF mRNA in skeletal muscle tissue taken by biopsy	24 hours
STARS mRNA in skeletal muscle tissue measured by Quantitative polymerase chain reaction (qPCR)	STARS mRNA in skeletal muscle tissue taken by biopsy	24 hoursn
Myogenin mRNA in skeletal muscle tissue measured by Quantitative polymerase chain reaction (qPCR)	Myogenin mRNA in skeletal muscle tissue taken by biopsy	24 hours
IL-6 (interleukin 6) mRNA in skeletal muscle tissue measured by Quantitative polymerase chain reaction (qPCR)	IL-6 mRNA in skeletal muscle tissue taken by biopsy	24 hours
PGC1a4 (Peroxisome proliferator-activated receptor gamma coactivator 1-alpha 4) mRNA in skeletal muscle tissue measured by Quantitative polymerase chain reaction (qPCR)	PGC1a4 mRNA in skeletal muscle tissue taken by biopsy	24 hours
PGC1a (Peroxisome proliferator-activated receptor gamma coactivator 1-alpha) mRNA in skeletal muscle tissue measured by Quantitative polymerase chain reaction (qPCR)	PGC1a mRNA in skeletal muscle tissue taken by biopsy	24 hours
Atrogin mRNA in skeletal muscle tissue measured by Quantitative polymerase chain reaction (qPCR)	Atrogin mRNA in skeletal muscle tissue taken by biopsy	24 hours
Beclin mRNA in skeletal muscle tissue measured by Quantitative polymerase chain reaction (qPCR)	Beclin mRNA in skeletal muscle tissue taken by biopsy	24 hours
Myostatin mRNA in skeletal muscle tissue measured by Quantitative polymerase chain reaction (qPCR)	Myostatin mRNA in skeletal muscle tissue taken by biopsy	24 hours

Collaborators and Investigators

• Sponsor: University of Padova
• Collaborators: University of Roma La Sapienza
• Investigators: Study Director: Antonio Paoli, MD, University of Padova

Publications and helpful links

General Publications

• Paoli A. Resistance training: the multifaceted side of exercise. Am J Physiol Endocrinol Metab. 2012 Feb 1;302(3):E387. doi: 10.1152/ajpendo.00541.2011. No abstract available.
• Paoli A, Moro T, Marcolin G, Neri M, Bianco A, Palma A, Grimaldi K. High-Intensity Interval Resistance Training (HIRT) influences resting energy expenditure and respiratory ratio in non-dieting individuals. J Transl Med. 2012 Nov 24;10:237. doi: 10.1186/1479-5876-10-237.
• Paoli A, Pacelli QF, Cancellara P, Toniolo L, Moro T, Canato M, Miotti D, Reggiani C. Myosin isoforms and contractile properties of single fibers of human Latissimus Dorsi muscle. Biomed Res Int. 2013;2013:249398. doi: 10.1155/2013/249398. Epub 2013 Jul 22.

Study record dates

Study Major Dates

• Study Start (ACTUAL): February 1, 2012
• Primary Completion (ACTUAL): May 15, 2012
• Study Completion (ACTUAL): February 10, 2013

Study Registration Dates

• First Submitted: March 19, 2020
• First Submitted That Met QC Criteria: March 24, 2020
• First Posted (ACTUAL): March 25, 2020

Study Record Updates

• Last Update Posted (ACTUAL): March 25, 2020
• Last Update Submitted That Met QC Criteria: March 24, 2020
• Last Verified: March 1, 2020

More Information

Terms related to this study

• Keywords: recovery; resistance training; muscle hypertrophy; muscle signalling
• Other Study ID Numbers: HIIRT12

Plan for Individual participant data (IPD)

• Plan to Share Individual Participant Data (IPD)?: NO

Drug and device information, study documents

• Studies a U.S. FDA-regulated drug product: No
• Studies a U.S. FDA-regulated device product: No