PTX-COVID19-B, an mRNA Humoral Vaccine, Intended for Prevention of COVID-19 in a General Population. This Study is Designed to Demonstrate the Safety, Tolerability, and Immunogenicity of PTX-COVID19-B in Comparison to the Pfizer-BioNTech COVID-19 Vaccine.

A Phase 2, Randomized, Double-dummy, Observer-Blind, Study to Evaluate the Safety, Tolerability, and Immunogenicity of PTX-COVID19-B Compared to Pfizer-BioNTech COVID-19 Vaccine in Healthy Seronegative Adults Aged 18 to 64 Years

The Vaccine Product, PTX-COVID19-B mRNA Humoral Vaccine, is intended for prevention of COVID-19 in a general population. This study is designed to evaluate the safety, tolerability, and immunogenicity of PTX-COVID19-B compared to Pfizer-BioNTech COVID-19 vaccine in healthy seronegative adults aged 18 to 64.

Study Overview

• Status: Completed
• Conditions: Covid19 Vaccine
• Intervention / Treatment: Biological: Placebo; Biological: PTX-COVID19-B; Biological: Pfizer-BioNTech COVID-19 vaccine

• Study Type: Interventional
• Enrollment (Actual): 565
• Phase: Phase 2

Contacts and Locations

Study Locations

• Canada
  • Alberta
    • Calgary, Alberta, Canada, T2H 2G4
      • LMC Clinical Research Inc. (Calgary)
  • Ontario
    • Mississauga, Ontario, Canada, L4V1P1
      • Malton Medical
    • Ottawa, Ontario, Canada, K1H 1E4
      • Red Maple
    • Toronto, Ontario, Canada, M1S3V6
      • Pharma Medica Research Inc.
    • Toronto, Ontario, Canada, M4G3E8
      • Manna Bayview
    • Toronto, Ontario, Canada, M9W4L6
      • Manna Toronto
• South Africa
  • Gauten
    • Johannesburg, Gauten, South Africa, 1864
      • Wits Vaccines & Infections Diseases Analytics (VIDA) Research Unit
  • Mpumalanga
    • Middleburg, Mpumalanga, South Africa, 1055
      • MERC Middleburg
  • Western Cape
    • Cape Town, Western Cape, South Africa, 7500
      • TREAD Research
    • Cape Town, Western Cape, South Africa, 7700
      • UCT Lung institute
    • Paarl, Western Cape, South Africa, 7626
      • Be Part Research

Participation Criteria

Eligibility Criteria

• Ages Eligible for Study: 18 years to 64 years (Adult)
• Accepts Healthy Volunteers: Yes
• Genders Eligible for Study: All

Description

Inclusion Criteria:

Each subject must meet the following criteria to be enrolled and randomized into the study:

1. Willing and able to provide written informed consent to participate prior to commencing any study-related activities.
2. Must be able to attend all visits (scheduled and unscheduled, as applicable) for the duration of the study and comply with all study procedures.
3. Healthy adult males and females 18 to 64 years of age, inclusive, at Screening Visit (Visit 1).
4. Negative to SARS-CoV-2 (COVID-19 disease) at screening and at each dosing, without evidence of recent of exposure or viral respiratory disease not identified as influenza or respiratory syncytial virus (RSV) (febrile or lower respiratory tract infection).
5. Must be in general good health before study participation with no clinically relevant abnormalities that could interfere with study assessments at investigator's discretion. Subjects may have stable comorbidities (no change in medications, exacerbations, or hospitalizations in past three (3) months).

6. Women of childbearing potential (WOCBP) and men whose sexual partners are WOCBP must be able and willing to use at least one (1) highly effective method of contraception (i.e., including hysterectomy, bilateral salpingectomy, and bilateral oophorectomy, hormonal oral [in combination with male condoms with spermicide], transdermal, implant, or injection, barrier [i.e., condom, diaphragm with spermicide]; intrauterine device; vasectomized partner [six months minimum], clinically sterile partner; or abstinence) during the study.

   • A female subject is considered a WOCBP after menarche and until she is in a postmenopausal state for 12 consecutive months (without an alternative medical cause) or otherwise permanently sterile.

   • Subjects not of childbearing potential are not required to use any other forms of contraception during the study. Non-childbearing potential is defined as subject confirmed:

     • Surgical sterilization (e.g., bilateral oophorectomy, bilateral salpingectomy, bilateral occlusion by cautery, hysterectomy, or tubal ligation).
     • Postmenopausal (defined as permanent cessation of menstruation for at least 12 consecutive months prior to screening); if postmenopausal status is unclear, pregnancy tests will be performed prior to vaccinations.

7. WOCBP must have a negative pregnancy test before each vaccination. If menopausal status is unclear, a pregnancy test is required.

   Inclusion into the open label safety extension

8. Receipt of at least one (1) vaccination prior to Day 42.

Exclusion Criteria:

Subjects will not be eligible for study participation if they meet any of the exclusion criteria:

1. History of an acute or chronic medical condition that, in the opinion of the investigator, would render vaccination unsafe or would interfere with the evaluation of responses.
2. History of ongoing clinical condition or medication or treatments that may adversely affect the immune system.
3. Subjects with pulse oximetry <90% at screening and prior to each vaccination.
4. Individuals who are antigen positive, seropositive or reverse transcriptase polymerase chain reaction (RT-PCR) positive for SARS-CoV-2, including prior to a second dose of PTX-COVID19-B.
5. Individuals who are at increased risk of exposure to SARS-CoV-2 (e.g., healthcare workers, emergency responders).
6. Close contact of anyone known to have SARS-CoV-2 infection within 30 days prior to vaccine administration.
7. Individuals with any elevated (Grade 1 or higher) laboratory test assessed as clinically significant for age/sex by the investigator at screening.
8. Individuals known to have anti-platelet antibodies.
9. Individuals with myocarditis and/or arrythmias.
10. Active neoplastic disease (excluding non-melanoma skin cancer that was successfully treated) or a history of any hematological malignancy. ("Active" is defined as having received treatment within the past five years).
11. Long-term (> two (2) weeks) use of oral or parenteral steroids or high-dose inhaled steroids (> 800 μg/day of beclomethasone dipropionate or equivalent) within six months before screening (nasal and topical steroids are allowed).
12. History of autoimmune, inflammatory disease, or potential immune-mediated medical conditions (PIMMCs).
13. Women currently pregnant, lactating, or planning a pregnancy between enrollment and Day 42.
14. History of immune thrombocytopenic purpura (ITP).
15. History of Guillain-Barré Syndrome or any degenerative neurology disorder.
16. History of anaphylactic-type reaction to any injected vaccines.
17. Known or suspected hypersensitivity to one or more of the components of the vaccine.
18. Known history of alcohol abuse, illicit drug use, physical dependence to any opioid, or any history of drug abuse or addiction within 12 months of screening.
19. Acute illness or fever (temperature >37.5C) within three (3) days before study enrollment (enrollment may be delayed for full recovery if acceptable to the investigator and within Protocol-defined windows).
20. Individuals currently participating or planning to participate in a study that involves an experimental agent (vaccine, drug, biologic, device, or medication); or who have received an experimental agent within one (1) month (three (3) months for immunoglobulins) before enrollment in this study; or who expect to receive another experimental agent up to Day 90 in this study.
21. Receipt of immunoglobulin or another blood product within the three (3) months before enrollment in this study or those who expect to receive immunoglobulin or another blood product during this study.
22. Individuals using prescription medications for prophylaxis of SARS-CoV-2.
23. Individuals who plan to receive another vaccine within the first three (3) months of the study (except influenza vaccine which should not be given within two (2) weeks of vaccine).
24. Receipt of any other SARS-CoV-2 or other experimental coronavirus (MERS, SARS etc.) vaccine at any time prior to or during the study.
25. Receipt of any investigational vaccine or investigational drug within one (1) month of enrollment and through the end of the study (one (1) year after the last vaccination).
26. Planning international travel from enrollment through Day 43.
27. History of surgery or major trauma within 12 weeks of screening, or surgery planned during the study.
28. Significant blood loss (> 400 mL) or has donated one or more units of blood (within 56 days for males and 84 days for females) or plasma (within two (2) weeks) prior to study participation.
29. Positive urine drugs of abuse screen test result.
30. Positive screen for HIV-1 and HIV-2 antibodies, HBsAg, or HCV antibody.
31. Involved in the planning or conduct of this study.

32. Unwilling or unlikely to comply with the requirements of the study.

    Exclusion from the open label safety extension:

33. Participation in a study that would affect potential immunogenicity evaluation during ongoing participation in this study. Participation in any other study is allowed after Day 90.

Study Plan

How is the study designed?

Design Details

• Primary Purpose: Prevention
• Allocation: Randomized
• Interventional Model: Parallel Assignment
• Masking: Quadruple

Number of Arms

3

Arms and Interventions

Participant Group / Arm	Intervention / Treatment
Experimental: 40µg PTX-COVID19-B Open-label; Participants, 15 healthy adults 18 to 64 years of age will receive 1 intramuscular (IM) injection of 40µg PTX-COVID19-B vaccine on Day 1, followed by a second dose on Day 28.	Biological: PTX-COVID19-B; Sterile solution for injection
Experimental: 40µg PTX-COVID19-B; Participants, 360 healthy adults 18 to 64 years of age, will receive 1 intramuscular (IM) injection of 40µg PTX-COVID19-B vaccine on Day 1, followed by a second dose on Day 28. Participants will receive a placebo dose on Day 21.	Biological: Placebo; 0.9% sodium chloride (normal saline) injection; Biological: PTX-COVID19-B; Sterile solution for injection
Active Comparator: Pfizer-BioNTech COVID-19 vaccine; Participants, 190 healthy adults 18 to 64 years of age, will receive 1 intramuscular (IM) injection of Pfizer-BioNTech COVID-19 vaccine on Day 1, followed by a placebo dose on Days 21. Participants will receive a placebo dose on Day 28.	Biological: Placebo; 0.9% sodium chloride (normal saline) injection; Biological: Pfizer-BioNTech COVID-19 vaccine; Sterile solution for injection

What is the study measuring?

Primary Outcome Measures

Outcome Measure	Measure Description	Time Frame
Injection Site Reactions	Injection site reactions (e.g., arm check evaluations including pain, tenderness, erythema/redness, induration/swelling) through the seventh day post each vaccination	Day 1 through Day 42
Incidence of Solicited Adverse Events (AEs)	Incidence of solicited adverse events (AEs) (e.g., fever, chills, nausea/vomiting, diarrhea, headache, fatigue, myalgia) through the seventh day post each vaccination and Serious Adverse Events (SAEs)	Day 1 through Day 7 for each dose administration
Assessment of AEs	Incidence of unsolicited AEs and SAEs	Day 1 through Day 42
Assessment of Safety	Medically attended AEs New onset chronic disease SAEs, AEs of special interest Potential immune-mediated medical conditions	Day 1 through Day 42

Collaborators and Investigators

• Sponsor: Providence Therapeutics Holdings Inc.

Study record dates

Study Major Dates

• Study Start (Actual): August 17, 2021
• Primary Completion (Actual): March 29, 2023
• Study Completion (Actual): March 29, 2023

Study Registration Dates

• First Submitted: December 30, 2021
• First Submitted That Met QC Criteria: December 30, 2021
• First Posted (Actual): January 4, 2022

Study Record Updates

• Last Update Posted (Actual): April 7, 2023
• Last Update Submitted That Met QC Criteria: April 5, 2023
• Last Verified: April 1, 2023

More Information

Terms related to this study

• Additional Relevant MeSH Terms: Coronavirus Infections; Coronaviridae Infections; Nidovirales Infections; RNA Virus Infections; Virus Diseases; Infections; Respiratory Tract Infections; Respiratory Tract Diseases; Pneumonia, Viral; Pneumonia; Lung Diseases; COVID-19
• Other Study ID Numbers: PRO-CL-002

Drug and device information, study documents

• Studies a U.S. FDA-regulated drug product: No
• Studies a U.S. FDA-regulated device product: No