- ICH GCP
- US Clinical Trials Registry
- Clinical Trial NCT05375149
Exhaled Breath Particles in Lung Transplantation
Exhaled Breath Particles as a Clinical Indicator for Acute and Chronic Rejection After Lung Transplantation
Lung transplantation (LTx) is the only effective treatment for patients with end stage lung disease. Of the major organs transplanted, survival following LTx is the lowest with a mean of 5 years. Despite improvements, primary graft dysfunction (PGD) remains the leading cause of early mortality and contributes to the development of chronic lung allograft dysfunction (CLAD) that remains the leading cause of late mortality. Earlier detection of rejection after LTx is of substantial importance as it would improve the possibilities of treatment and could increase survival.
The investigators have shown in previous work that exhaled breath particles (EBP) reflect the composition of respiratory tract lining fluid (RTLF). EBP and particle flow rate (PFR) can be used as non-invasive methods for early detection and monitoring of airway diseases such as acute respiratory distress syndrome (ARDS). It has also been shown that the particle flow prolife after lung transplantation differs between patients who develop PGD and those who do not and that the composition of EBP differs between patients with and without bronchiolitis obliterans syndrome (BOS), an obstructive form of CLAD.
Samples of EBP and measurements of PFR will be collected from lung transplanted patients. Membranes with EBP will be saved for molecular analysis. The investigators aim to identify potential particle flow patterns and biomarkers for earlier detection of rejection after lung transplantation.
Study Overview
Status
Detailed Description
Samples of EBP and measurement of PFR will be done on patients undergoing lung transplantation at Skåne University Hospital (SUS) Lund. Measurements of EBP and PFR and collection of blood will be done repeatedly postoperatively when the patient is still in the hospital, at three months post-transplantation, at six months post-transplantation, at 12 months post-transplantation and annually after that. An additional preoperative blood sample will also be obtained. Furthermore, the investigators will collect bronchoalveolar lavage fluid (BALF) and lung biopsies when the patient is scheduled for routine bronchoscopies in the follow-up period.
This study involves measurements of EBP and PFR done by the particles in exhaled air (PExA) system on lung transplanted patients with and without acute or chronic rejection. The patients who do not develop any form of rejection will serve as a control group to the ones who develop rejection. Furthermore, each patient will serve as their own control.
The purpose of this study is to find better means of identifying rejection of transplanted lungs in earlier stages, and to explore candidate biomarkers. Earlier diagnosis of rejection of the transplanted organ can lead to better treatment possibilities and a positive impact on survival after LTx.
Study Type
Enrollment (Anticipated)
Contacts and Locations
Study Contact
- Name: Sandra Lindstedt, MD, PhD
- Phone Number: +46737220580
- Email: sandra.lindstedt_ingemansson@med.lu.se
Study Locations
-
-
Skåne Län
-
Lund, Skåne Län, Sweden, 224 60
- Recruiting
- Skåne University Hospital
-
-
Participation Criteria
Eligibility Criteria
Ages Eligible for Study
Accepts Healthy Volunteers
Genders Eligible for Study
Sampling Method
Study Population
Description
Inclusion Criteria:
- Patients who have undergone LTx at Skåne University Hospital, SUS Lund
Exclusion Criteria:
- None
Study Plan
How is the study designed?
Design Details
- Observational Models: Case-Control
- Time Perspectives: Prospective
Cohorts and Interventions
Group / Cohort |
---|
LTx rejection
Lung transplanted patients with acute or chronic rejection
|
LTx non-rejection
Lung transplanted patients without any form of rejection
|
What is the study measuring?
Primary Outcome Measures
Outcome Measure |
Measure Description |
Time Frame |
---|---|---|
Concentration of proteins in EBP
Time Frame: Pre-transplantation
|
Proteins from exhaled air are collected onto a membrane for subsequently molecular analysis.
Analysis aims to identify candidate biomarker for acute and chronic rejection of transplanted lungs.
|
Pre-transplantation
|
Concentration of proteins in EBP
Time Frame: Day 1-3 after lung transplantation
|
Proteins from exhaled air are collected onto a membrane for subsequently molecular analysis.
Analysis aims to identify candidate biomarker for acute and chronic rejection of transplanted lungs.
|
Day 1-3 after lung transplantation
|
Concentration of proteins in EBP
Time Frame: 1 month after lung transplantation
|
Proteins from exhaled air are collected onto a membrane for subsequently molecular analysis.
Analysis aims to identify candidate biomarker for acute and chronic rejection of transplanted lungs.
|
1 month after lung transplantation
|
Concentration of proteins in EBP
Time Frame: 3 months after lung transplantation
|
Proteins from exhaled air are collected onto a membrane for subsequently molecular analysis.
Analysis aims to identify candidate biomarker for acute and chronic rejection of transplanted lungs.
|
3 months after lung transplantation
|
Concentration of proteins in EBP
Time Frame: 6 months after lung transplantation
|
Proteins from exhaled air are collected onto a membrane for subsequently molecular analysis.
Analysis aims to identify candidate biomarker for acute and chronic rejection of transplanted lungs.
|
6 months after lung transplantation
|
Concentration of proteins in EBP
Time Frame: 9 months after lung transplantation
|
Proteins from exhaled air are collected onto a membrane for subsequently molecular analysis.
Analysis aims to identify candidate biomarker for acute and chronic rejection of transplanted lungs.
|
9 months after lung transplantation
|
Concentration of proteins in EBP
Time Frame: 12 months after lung transplantation
|
Proteins from exhaled air are collected onto a membrane for subsequently molecular analysis.
Analysis aims to identify candidate biomarker for acute and chronic rejection of transplanted lungs.
|
12 months after lung transplantation
|
Concentration of proteins in EBP
Time Frame: 18 months after lung transplantation
|
Proteins from exhaled air are collected onto a membrane for subsequently molecular analysis.
Analysis aims to identify candidate biomarker for acute and chronic rejection of transplanted lungs.
|
18 months after lung transplantation
|
Concentration of proteins in EBP
Time Frame: 2 years after lung transplantation
|
Proteins from exhaled air are collected onto a membrane for subsequently molecular analysis.
Analysis aims to identify candidate biomarker for acute and chronic rejection of transplanted lungs.
|
2 years after lung transplantation
|
Concentration of proteins in EBP
Time Frame: 3 years after lung transplantation
|
Proteins from exhaled air are collected onto a membrane for subsequently molecular analysis.
Analysis aims to identify candidate biomarker for acute and chronic rejection of transplanted lungs.
|
3 years after lung transplantation
|
Concentration of proteins in EBP
Time Frame: 4 years after lung transplantation
|
Proteins from exhaled air are collected onto a membrane for subsequently molecular analysis.
Analysis aims to identify candidate biomarker for acute and chronic rejection of transplanted lungs.
|
4 years after lung transplantation
|
Particle flow rate from the airways (PFR)
Time Frame: Day 1-3 after transplantation
|
PFR will be measured by the PExA device.
The flow rate and the particle pattern will be analysed to find differences between groups of lung transplanted patients with and without rejection.
|
Day 1-3 after transplantation
|
Particle flow rate from the airways (PFR)
Time Frame: 1 month after transplantation
|
PFR will be measured by the PExA device.
The flow rate and the particle pattern will be analysed to find differences between groups of lung transplanted patients with and without rejection.
|
1 month after transplantation
|
Particle flow rate from the airways (PFR)
Time Frame: 3 months after transplantation
|
PFR will be measured by the PExA device.
The flow rate and the particle pattern will be analysed to find differences between groups of lung transplanted patients with and without rejection.
|
3 months after transplantation
|
Particle flow rate from the airways (PFR)
Time Frame: 6 months after transplantation
|
PFR will be measured by the PExA device.
The flow rate and the particle pattern will be analysed to find differences between groups of lung transplanted patients with and without rejection.
|
6 months after transplantation
|
Particle flow rate from the airways (PFR)
Time Frame: 9 months after transplantation
|
PFR will be measured by the PExA device.
The flow rate and the particle pattern will be analysed to find differences between groups of lung transplanted patients with and without rejection.
|
9 months after transplantation
|
Particle flow rate from the airways (PFR)
Time Frame: 12 months after transplantation
|
PFR will be measured by the PExA device.
The flow rate and the particle pattern will be analysed to find differences between groups of lung transplanted patients with and without rejection.
|
12 months after transplantation
|
Particle flow rate from the airways (PFR)
Time Frame: 18 months after transplantation
|
PFR will be measured by the PExA device.
The flow rate and the particle pattern will be analysed to find differences between groups of lung transplanted patients with and without rejection.
|
18 months after transplantation
|
Particle flow rate from the airways (PFR)
Time Frame: 2 years after transplantation
|
PFR will be measured by the PExA device.
The flow rate and the particle pattern will be analysed to find differences between groups of lung transplanted patients with and without rejection.
|
2 years after transplantation
|
Particle flow rate from the airways (PFR)
Time Frame: 3 years after transplantation
|
PFR will be measured by the PExA device.
The flow rate and the particle pattern will be analysed to find differences between groups of lung transplanted patients with and without rejection.
|
3 years after transplantation
|
Particle flow rate from the airways (PFR)
Time Frame: 4 years after transplantation
|
PFR will be measured by the PExA device.
The flow rate and the particle pattern will be analysed to find differences between groups of lung transplanted patients with and without rejection.
|
4 years after transplantation
|
Secondary Outcome Measures
Outcome Measure |
Measure Description |
Time Frame |
---|---|---|
Concentration of proteins and other biomarkers in plasma
Time Frame: Pre-transplantation
|
Concentration and composition of proteins and other biomarkers will be analysed in plasma
|
Pre-transplantation
|
Concentration of proteins and other biomarkers in plasma
Time Frame: Day 1 after lung transplantation
|
Concentration and composition of proteins and other biomarkers will be analysed in plasma
|
Day 1 after lung transplantation
|
Concentration of proteins and other biomarkers in plasma
Time Frame: Day 2 after lung transplantation
|
Concentration and composition of proteins and other biomarkers will be analysed in plasma
|
Day 2 after lung transplantation
|
Concentration of proteins and other biomarkers in plasma
Time Frame: Day 3 after lung transplantation
|
Concentration and composition of proteins and other biomarkers will be analysed in plasma
|
Day 3 after lung transplantation
|
Concentration of proteins and other biomarkers in plasma
Time Frame: 1 month after lung transplantation
|
Concentration and composition of proteins and other biomarkers will be analysed in plasma
|
1 month after lung transplantation
|
Concentration of proteins and other biomarkers in plasma
Time Frame: 3 months after lung transplantation
|
Concentration and composition of proteins and other biomarkers will be analysed in plasma
|
3 months after lung transplantation
|
Concentration of proteins and other biomarkers in plasma
Time Frame: 6 months after lung transplantation
|
Concentration and composition of proteins and other biomarkers will be analysed in plasma
|
6 months after lung transplantation
|
Concentration of proteins and other biomarkers in plasma
Time Frame: 9 months after lung transplantation
|
Concentration and composition of proteins and other biomarkers will be analysed in plasma
|
9 months after lung transplantation
|
Concentration of proteins and other biomarkers in plasma
Time Frame: 12 months after lung transplantation
|
Concentration and composition of proteins and other biomarkers will be analysed in plasma
|
12 months after lung transplantation
|
Concentration of proteins and other biomarkers in plasma
Time Frame: 18 months after lung transplantation
|
Concentration and composition of proteins and other biomarkers will be analysed in plasma
|
18 months after lung transplantation
|
Concentration of proteins and other biomarkers in plasma
Time Frame: 2 years after lung transplantation
|
Concentration and composition of proteins and other biomarkers will be analysed in plasma
|
2 years after lung transplantation
|
Concentration of proteins and other biomarkers in plasma
Time Frame: 3 years after lung transplantation
|
Concentration and composition of proteins and other biomarkers will be analysed in plasma
|
3 years after lung transplantation
|
Concentration of proteins and other biomarkers in plasma
Time Frame: 4 years after lung transplantation
|
Concentration and composition of proteins and other biomarkers will be analysed in plasma
|
4 years after lung transplantation
|
Concentration of proteins and other biomarkers in BAL
Time Frame: Day 1-3 after lung transplantation
|
Concentration and composition of proteins and other biomarkers will be analysed in BAL
|
Day 1-3 after lung transplantation
|
Concentration of proteins and other biomarkers in BAL
Time Frame: 1 month after lung transplantation
|
Concentration and composition of proteins and other biomarkers will be analysed in BAL
|
1 month after lung transplantation
|
Concentration of proteins and other biomarkers in BAL
Time Frame: 3 months after lung transplantation
|
Concentration and composition of proteins and other biomarkers will be analysed in BAL
|
3 months after lung transplantation
|
Concentration of proteins and other biomarkers in BAL
Time Frame: 6 months after lung transplantation
|
Concentration and composition of proteins and other biomarkers will be analysed in BAL
|
6 months after lung transplantation
|
Concentration of proteins and other biomarkers in BAL
Time Frame: 9 months after lung transplantation
|
Concentration and composition of proteins and other biomarkers will be analysed in BAL
|
9 months after lung transplantation
|
Concentration of proteins and other biomarkers in BAL
Time Frame: 12 months after lung transplantation
|
Concentration and composition of proteins and other biomarkers will be analysed in BAL
|
12 months after lung transplantation
|
Concentration of proteins and other biomarkers in BAL
Time Frame: 18 months after lung transplantation
|
Concentration and composition of proteins and other biomarkers will be analysed in BAL
|
18 months after lung transplantation
|
Concentration of proteins and other biomarkers in BAL
Time Frame: 2 years after lung transplantation
|
Concentration and composition of proteins and other biomarkers will be analysed in BAL
|
2 years after lung transplantation
|
Concentration of proteins and other biomarkers in BAL
Time Frame: 3 years after lung transplantation
|
Concentration and composition of proteins and other biomarkers will be analysed in BAL
|
3 years after lung transplantation
|
Concentration of proteins and other biomarkers in BAL
Time Frame: 4 years after lung transplantation
|
Concentration and composition of proteins and other biomarkers will be analysed in BAL
|
4 years after lung transplantation
|
Concentration of proteins and other biomarkers in lung tissue
Time Frame: At the time of lung transplantation
|
Concentration and composition of proteins and other biomarkers will be analysed in lung tissue.
Both taken from the native lung and from the donor lung.
|
At the time of lung transplantation
|
Concentration of proteins and other biomarkers in lung tissue
Time Frame: 1 months after lung transplantation
|
Concentration and composition of proteins and other biomarkers will be analysed in lung tissue
|
1 months after lung transplantation
|
Concentration of proteins and other biomarkers in lung tissue
Time Frame: 3 months after lung transplantation
|
Concentration and composition of proteins and other biomarkers will be analysed in lung tissue
|
3 months after lung transplantation
|
Concentration of proteins and other biomarkers in lung tissue
Time Frame: 6 months after lung transplantation
|
Concentration and composition of proteins and other biomarkers will be analysed in lung tissue
|
6 months after lung transplantation
|
Concentration of proteins and other biomarkers in lung tissue
Time Frame: 9 months after lung transplantation
|
Concentration and composition of proteins and other biomarkers will be analysed in lung tissue
|
9 months after lung transplantation
|
Concentration of proteins and other biomarkers in lung tissue
Time Frame: 12 months after lung transplantation
|
Concentration and composition of proteins and other biomarkers will be analysed in lung tissue
|
12 months after lung transplantation
|
Concentration of proteins and other biomarkers in lung tissue
Time Frame: 18 months after lung transplantation
|
Concentration and composition of proteins and other biomarkers will be analysed in lung tissue
|
18 months after lung transplantation
|
Concentration of proteins and other biomarkers in lung tissue
Time Frame: 2 years after lung transplantation
|
Concentration and composition of proteins and other biomarkers will be analysed in lung tissue
|
2 years after lung transplantation
|
Concentration of proteins and other biomarkers in lung tissue
Time Frame: 3 years after lung transplantation
|
Concentration and composition of proteins and other biomarkers will be analysed in lung tissue
|
3 years after lung transplantation
|
Concentration of proteins and other biomarkers in lung tissue
Time Frame: 4 years after lung transplantation
|
Concentration and composition of proteins and other biomarkers will be analysed in lung tissue
|
4 years after lung transplantation
|
Collaborators and Investigators
Sponsor
Investigators
- Principal Investigator: Sandra Lindstedt, MD, PhD, Region Skåne, Lund University
Publications and helpful links
General Publications
- Ericson PA, Mirgorodskaya E, Hammar OS, Viklund EA, Almstrand AR, Larsson PJ, Riise GC, Olin AC. Low Levels of Exhaled Surfactant Protein A Associated With BOS After Lung Transplantation. Transplant Direct. 2016 Aug 26;2(9):e103. doi: 10.1097/TXD.0000000000000615. eCollection 2016 Sep.
- Stenlo M, Hyllen S, Silva IAN, Bolukbas DA, Pierre L, Hallgren O, Wagner DE, Lindstedt S. Increased particle flow rate from airways precedes clinical signs of ARDS in a porcine model of LPS-induced acute lung injury. Am J Physiol Lung Cell Mol Physiol. 2020 Mar 1;318(3):L510-L517. doi: 10.1152/ajplung.00524.2019. Epub 2020 Jan 29.
- Broberg E, Hyllen S, Algotsson L, Wagner DE, Lindstedt S. Particle Flow Profiles From the Airways Measured by PExA Differ in Lung Transplant Recipients Who Develop Primary Graft Dysfunction. Exp Clin Transplant. 2019 Dec;17(6):803-812. doi: 10.6002/ect.2019.0187. Epub 2019 Oct 11.
- Behndig AF, Mirgorodskaya E, Blomberg A, Olin AC. Surfactant Protein A in particles in exhaled air (PExA), bronchial lavage and bronchial wash - a methodological comparison. Respir Res. 2019 Sep 26;20(1):214. doi: 10.1186/s12931-019-1172-1.
Study record dates
Study Major Dates
Study Start (Actual)
Primary Completion (Anticipated)
Study Completion (Anticipated)
Study Registration Dates
First Submitted
First Submitted That Met QC Criteria
First Posted (Actual)
Study Record Updates
Last Update Posted (Actual)
Last Update Submitted That Met QC Criteria
Last Verified
More Information
Terms related to this study
Keywords
Additional Relevant MeSH Terms
Other Study ID Numbers
- LTxPEx
Plan for Individual participant data (IPD)
Plan to Share Individual Participant Data (IPD)?
Drug and device information, study documents
Studies a U.S. FDA-regulated drug product
Studies a U.S. FDA-regulated device product
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