Effect of Beet-root Juice and PBM Treatments on Muscle Fatigue

Application of Beet-root Juice Supplementation and Photobiomodulation Treatment on Forearm Muscle Fatigue in Older Adults

The goal of this randomized counterbalanced repeated measures study is to test beet-root juice supplementation and red light therapy augment forearm muscle endurance in apparently healthy older adults over 65 years of age. The main questions seek to answer the following question:

Does beet-root juice supplementation improve forearm muscle endurance compared to a placebo supplement?

Does red light therapy improve forearm muscle endurance compared to a sham light exposure?

Does beet-root juice supplementation in combination with red light therapy improve forearm muscle endurance compared to a placebo-sham light control?

Study Overview

• Status: Recruiting
• Conditions: Healthy Aging; Health Care Utilization
• Intervention / Treatment: Dietary supplement: Beet-root juice; Radiation: red-light therapy; Radiation: sham light; Dietary supplement: Placebo

• Study Type: Interventional
• Enrollment (Estimated): 10
• Phase: Not Applicable

Contacts and Locations

• Study Contact: Name: Andrew D Wells, Ph.D.; Phone Number: 2156 (336) 758-5837; Email: wellsa@wfu.edu

Study Locations

• United States
  • North Carolina
    • Winston-Salem, North Carolina, United States, 27106
      • Recruiting
      • Wake Forest Univesity
      • Contact: Andrew D Wells, Ph.D.; Phone Number: 2156 336-758-5837; Email: wellsa@wfu.edu

Participation Criteria

Eligibility Criteria

• Ages Eligible for Study: 65 years and older (Older Adult)
• Accepts Healthy Volunteers: Yes

Description

Inclusion Criteria:

• Self-reports performing at least 30 minutes of light to moderate intensity physical activity 3 times per week for the last three months

Exclusion Criteria:

• Currently smoke
• Have smoked within the last 6 months
• Are taking taking supplements known to increase blood nitrate levels
• Have a known kidney disease
• Have a known liver disease
• Have a known thyroid condition
• Have a known gastritis condition
• Are taking medications that are known to cause complications with nitrate supplementation
• Have known or reported joint pain within the dominant hand

Study Plan

How is the study designed?

Design Details

• Primary Purpose: Basic Science
• Allocation: Randomized
• Interventional Model: Crossover Assignment
• Masking: Single

Number of Arms

4

Arms and Interventions

Participant Group / Arm	Intervention / Treatment
Experimental: Beet-root juice plus red-light therapy	Dietary supplement: Beet-root juice; Two days prior to the forearm endurance protocol, participants will be provided and instructed to consume a 400mg nitrate once per day. On the day of the forearm endurance protocol, the participants will be provided and instructed to consume a third 400mg dose of nitrates at least 150 minutes before forearm endurance testing.; Other Names: Beet it Sport; Radiation: red-light therapy; Participants will place their dominant forearm 15 centimeters away from the light source and have their dominant forearm exposed to 660nm wave length light for 10-minutes.; Other Names: MitoMIN
Sham Comparator: Beet-root juice plus sham light therapy	Dietary supplement: Beet-root juice; Two days prior to the forearm endurance protocol, participants will be provided and instructed to consume a 400mg nitrate once per day. On the day of the forearm endurance protocol, the participants will be provided and instructed to consume a third 400mg dose of nitrates at least 150 minutes before forearm endurance testing.; Other Names: Beet it Sport; Radiation: sham light; Participants will place their dominant forearm 15 centimeters away from the light source and have their dominant forearm exposed to sham light for 10-minutes.
Placebo Comparator: Placebo plus red-light therapy	Radiation: red-light therapy; Participants will place their dominant forearm 15 centimeters away from the light source and have their dominant forearm exposed to 660nm wave length light for 10-minutes.; Other Names: MitoMIN; Dietary supplement: Placebo; Two days prior to the forearm endurance protocol, participants will be provided and instructed to consume a nitrate depleted placebo once per day. On the day of the forearm endurance protocol, the participants will be provided and instructed to consume a third dose of the nitrate depleted placebo at least 150 minutes before forearm endurance testing.
Active Comparator: Placebo plus sham light therapy	Radiation: sham light; Participants will place their dominant forearm 15 centimeters away from the light source and have their dominant forearm exposed to sham light for 10-minutes.; Dietary supplement: Placebo; Two days prior to the forearm endurance protocol, participants will be provided and instructed to consume a nitrate depleted placebo once per day. On the day of the forearm endurance protocol, the participants will be provided and instructed to consume a third dose of the nitrate depleted placebo at least 150 minutes before forearm endurance testing.

What is the study measuring?

Primary Outcome Measures

Outcome Measure	Measure Description	Time Frame
Total forearm work performed	Work is the product of the number of times the forearm is activated multiplied by the amount of force produced by the forearm in kilograms.	During the forearm exercise fatigue protocol lasting up to 45 minutes

Secondary Outcome Measures

Outcome Measure	Measure Description	Time Frame
Forearm endurance	The duration of time the participant takes to complete the forearm fatigue protocol	During the forearm exercise fatigue protocol lasting up to 45 minutes
Intermuscular coordination	Surface electromyography measurements of muscle activity	During the forearm exercise fatigue protocol lasting up to 45 minutes

Collaborators and Investigators

• Sponsor: Wake Forest University
• Investigators: Principal Investigator: Andrew D Wells, Ph.D., Wake Forest University, Reynolda Campus

Study record dates

Study Major Dates

• Study Start (Actual): September 9, 2022
• Primary Completion (Estimated): August 31, 2024
• Study Completion (Estimated): August 31, 2024

Study Registration Dates

• First Submitted: October 21, 2022
• First Submitted That Met QC Criteria: October 26, 2022
• First Posted (Actual): October 27, 2022

Study Record Updates

• Last Update Posted (Actual): March 20, 2024
• Last Update Submitted That Met QC Criteria: March 19, 2024
• Last Verified: March 1, 2024

More Information

Terms related to this study

• Keywords: Electromyography; Red light therapy; Forearm muscle endurance; Beet-root juice
• Additional Relevant MeSH Terms: Fatigue
• Other Study ID Numbers: IRB00024675

Drug and device information, study documents

• Studies a U.S. FDA-regulated drug product: No
• Studies a U.S. FDA-regulated device product: Yes
• product manufactured in and exported from the U.S.: No