Inflamation, Gut Leakage, Iron Status After 6000m Ergometer Test in Elite Rowers. (6000m)

Influance of 6000m Ergometer Test on Inflammatory, Permeability Markers and Iron Status.

The influence of a 6000m ergometer test on the markers of gut injury, endotoxemia, iron status, stress and inflammation in elite rowers.

Study Overview

• Status: Completed
• Conditions: Oxidative Stress; Iron Status; Endothenial Dysfunction
• Intervention / Treatment: Other: exercise test

Detailed Description

The principal objective of our study was to assess gut permeability after completing a 6000-meter rowing test. The study was performed at the beginning of a competitive training phase. Eighteen elite Polish Rowing Team rowers participated in the study after applying the inclusion/exclusion criteria. The participants performed a 6000-meter ergometer test. Blood samples were taken before the test, after exercise, and after 1 hour of restitution. Parameters, such as I-FABP (intestinal fatty acid binding protein), LPS (lipopolysaccharide), LBP (lipopolysaccharide binding protein), claudin3, ferritin, transferrin, hepcidin, IL-6 (Interleukin-6), HO-1 (Heme Oxygenase 1), and ACSL4 (Long-chain-fatty-acid-CoA ligase 4 ) were determined using appropriate biochemical tests.

• Study Type: Observational
• Enrollment (Actual): 21

Contacts and Locations

Study Locations

• Poland
  • Lubuskie
    • Gorzów Wielkopolski, Lubuskie, Poland, 66-400
      • Poznan University of Physical Education

Participation Criteria

Eligibility Criteria

• Ages Eligible for Study: Adult
• Accepts Healthy Volunteers: Yes

• Sampling Method: Non-Probability Sample

Study Population

Male rowers age 18 - 24 years.

Description

Inclusion Criteria:

• minimum five years of training,
• five training sessions per week minimum,
• total training time minimum of 240 minutes,
• membership in the Polish Rowing Team,
• finishing the 6000-meter ergometer test.

Exclusion Criteria:

• antibiotic therapy within the last three months,
• probiotics and prebiotics within the last three months,
• dietary regime,
• gastrointestinal diseases.

Study Plan

How is the study designed?

Number of groups / cohorts

1

Cohorts and Interventions

Group / Cohort	Intervention / Treatment
elite rowers	Other: exercise test; Participant perforemed 6000m ergometer test.

What is the study measuring?

Primary Outcome Measures

Outcome Measure	Measure Description	Time Frame
I-FABP (intestinal fatty acid binding protein)to measure epithelial wall injury	I-FABP concentration measured in blood [ng/ml] using commercially available enzyme-linked immunosorbent assays	Blood samples was taken from the cubital vein at three-time points: before each stress test (after overnight fasting), one minute after the end of the test, and after a 1-hour recovery period
LBP (lipopolysaccharide binding protein) to measure endotoxin	LBP concentration measured in blood [ug/ml] using commercially available enzyme-linked immunosorbent assays	Blood samples was taken from the cubital vein at three-time points: before each stress test (after overnight fasting), one minute after the end of the test, and after a 1-hour recovery period
LPS (lipopolysaccharide) to measure endotoxin	LPS concentration measured in blood [EU/Ll] using commercially available enzyme-linked immunosorbent assays	Blood samples was taken from the cubital vein at three-time points: before each stress test (after overnight fasting), one minute after the end of the test, and after a 1-hour recovery period
Lactic acid to measure fatigue after the race	Lactate concentration measured in capillary blood from earlobe [mmol/L] using a commercially available kit (Diaglobal, Berlin, Germany)	Blood samples was taken from earlobe at two-time points: before each stress test (after overnight fasting), one minute after the end of the test.
Claudin 3 to measure tight junction leakage	claudin 3 concentration measured in blood [ng/ml] using commercially available enzyme-linked immunosorbent assays	Blood samples was taken from the cubital vein at three-time points: before each stress test (after overnight fasting), one minute after the end of the test, and after a 1-hour recovery period
Ferritin to measure iron status	Ferritin concentration measured in blood [ng/ml] using commercially available enzyme-linked immunosorbent assays	Blood samples will be taken from the cubital vein at three-time points: before each stress test (after overnight fasting), one minute after the end of the test, and after a 1-hour recovery period.
Transferrin to measure iron status	Transferrin concentration measured in blood [mg/ml] using commercially available enzyme-linked immunosorbent assays	Blood samples will be taken from the cubital vein at three-time points: before each stress test (after overnight fasting), one minute after the end of the test, and after a 1-hour recovery period.
Hepcidin to measure iron status	Hepcidin concentration measured in blood [ng/ml] using commercially available enzyme-linked immunosorbent assays	Blood samples will be taken from the cubital vein at three-time points: before each stress test (after overnight fasting), one minute after the end of the test, and after a 1-hour recovery period.
IL-6 (Interleukin-6) to measure inflammation	IL-6 concentration measured in blood [ng/l] using commercially available enzyme-linked immunosorbent assays.	Blood samples will be taken from the cubital vein at three-time points: before each stress test (after overnight fasting), one minute after the end of the test, and after a 1-hour recovery period.
HO-1 (Heme Oxygenase 1) to measure oxidative stress	HO-1 concentration measured in blood [ng/ml] using commercially available enzyme-linked immunosorbent assays	Blood samples will be taken from the cubital vein at three-time points: before each stress test (after overnight fasting), one minute after the end of the test, and after a 1-hour recovery period.
ACSL4 (Long-chain-fatty-acid-CoA ligase 4 ) to measure oxidative stress	ACSL4 concentration measured in blood [pg/ml] using commercially available enzyme-linked immunosorbent assays.	Blood samples will be taken from the cubital vein at three-time points: before each stress test (after overnight fasting), one minute after the end of the test, and after a 1-hour recovery period.

Secondary Outcome Measures

Outcome Measure	Measure Description	Time Frame
body mass	Body mass [kg] measured by electronic scale to the nearest 0,05 kg (Tanita BC 418 MA Tanita Corporation, Tokyo, Japan).	in the morning before the test
Body composition - water	% of body water measured by electronic scale (Tanita BC 418 MA Tanita Corporation, Tokyo, Japan).	in the morning before the test
Body composition - fat	% of body fat measured by electronic scale (Tanita BC 418 MA Tanita Corporation, Tokyo, Japan).	in the morning before the test
energy	energy [kcal ]of food intake measured by 24h dietary recall	day before test, in the morning before the test
protein	protein [g ]of food intake measured by 24h dietary recall	day before test, in the morning before the test
carbohydrate	carbohydrate [g ]of food intake measured by 24h dietary recall	day before test, in the morning before the test
fat	fat [g ]of food intake measured by 24h dietary recall	day before test, in the morning before the test
fiber	fiber [g ]of food intake measured by 24h dietary recall	day before test, in the morning before the test
Lean body mass	Lean body mass [kg] measured by electronic scale to the nearest 0,05 kg (Tanita BC 418 MA Tanita Corporation, Tokyo, Japan).	in the morning before the test
Height	Prior to the exercise test, we measured the anthropometric parameters, including height (Seca 213 Hamburg, Deutschland) [cm]	in the morning before the test
Gastrointestinal sypthomps self reported scale	10 point scale were 10 is a outcome of the worst severity	up to 1 hours after the test

Collaborators and Investigators

• Sponsor: Poznan University of Physical Education

Study record dates

Study Major Dates

• Study Start (Actual): November 1, 2023
• Primary Completion (Actual): November 10, 2023
• Study Completion (Actual): November 10, 2023

Study Registration Dates

• First Submitted: March 17, 2025
• First Submitted That Met QC Criteria: March 17, 2025
• First Posted (Actual): March 25, 2025

Study Record Updates

• Last Update Posted (Actual): March 27, 2025
• Last Update Submitted That Met QC Criteria: March 24, 2025
• Last Verified: November 1, 2023

More Information

Terms related to this study

• Keywords: gut permeability; oxidative stress; iron status; endotoxin; gastrointestinal function
• Other Study ID Numbers: Rowers6000m 2023

Plan for Individual participant data (IPD)

• Plan to Share Individual Participant Data (IPD)?: NO

Drug and device information, study documents

• Studies a U.S. FDA-regulated drug product: No
• Studies a U.S. FDA-regulated device product: No
• product manufactured in and exported from the U.S.: No