Linking Dietary Nutrition, Dietary Patterns, Gut Microbiota, and Bone Density: A Cross-Sectional Study

November 29, 2025 updated by: Jianhua Ma

Investigating the Effects of Diet and Gut Microecology on Bone Health in Gannan Tibetan Elementary School Students

The study aims to understand the current situation of bone health in the Gannan Tibetan primary school student population, to explore the relationship between nutrition, intestinal microorganisms and bone health, to analyze the main factors affecting bone health from the perspective of nutrition and intestinal microecology, and to provide theoretical basis for the promotion and improvement of bone health in Tibetan primary school students.

Study Overview

Status

Completed

Conditions

Intervention / Treatment

Study Type

Observational

Enrollment (Actual)

431

Contacts and Locations

This section provides the contact details for those conducting the study, and information on where this study is being conducted.

Study Locations

  • China
    • Gansu
      • Lanzhou, Gansu, China, 730000
        • LanZhou University

Participation Criteria

Researchers look for people who fit a certain description, called eligibility criteria. Some examples of these criteria are a person's general health condition or prior treatments.

Eligibility Criteria

Ages Eligible for Study

  • Child

Accepts Healthy Volunteers

Yes

Sampling Method

Probability Sample

Study Population

This study is based on a cross-sectional design. Two schools were selected: one rural primary school in Xiahe County, Gannan Tibetan Autonomous Prefecture, and one urban primary school in Hezuo City, Gansu Province. Within the selected schools, a stratified random cluster sampling method was used to randomly select one class from each grade as the study subjects.

Description

Inclusion Criteria:

  • Tibetan primary school students currently enrolled in schools in Gannan Tibetan Autonomous Prefecture;

Willing to participate and able to cooperate in completing bone density measurements;

Additional inclusion criteria: Subjects who are 9 years old or older and agree to participate in dietary surveys and fecal sampling.

Exclusion Criteria:Use of corticosteroid medications within the past year;

Suffering from skeletal system diseases, metabolic disorders, or endocrine diseases;

Additional exclusion criteria: Subjects who have used antibiotics in the past three months and are undergoing fecal sampling.

-

Study Plan

This section provides details of the study plan, including how the study is designed and what the study is measuring.

How is the study designed?

Design Details

Cohorts and Interventions

Group / Cohort
Intervention / Treatment
Good Bone Density group and normal bone density group
Other: No Intervention: Observational Cohort
No Intervention

What is the study measuring?

Primary Outcome Measures

Outcome Measure
Measure Description
Time Frame
Dietary Characteristics
Time Frame: Day 1
Dietary data were collected using a food frequency questionnaire (FFQ) to analyze dietary patterns and nutrient intake.
Day 1
Bone density test
Time Frame: Day1
In this study, a peripheral dual-energy X-ray absorptiometry (EXA-PRESTO) was used to measure the bone density of the non-dominant forearm of the subjects.
Day1
Fecal sample microbiological testing and analysis
Time Frame: Day 1
This study entrusted Tianhao Gene Technology Co., Ltd. to perform the intestinal microbiome Accu16STM (16S rRNA absolute quantitative sequencing). The main research processes include DNA extraction, PCR amplification, library construction, and sequencing.
Day 1

Collaborators and Investigators

This is where you will find people and organizations involved with this study.

Sponsor

Jianhua Ma

Study record dates

These dates track the progress of study record and summary results submissions to ClinicalTrials.gov. Study records and reported results are reviewed by the National Library of Medicine (NLM) to make sure they meet specific quality control standards before being posted on the public website.

Study Major Dates

Study Start (Actual)

October 10, 2023

Primary Completion (Actual)

March 31, 2025

Study Completion (Actual)

March 31, 2025

Study Registration Dates

First Submitted

November 20, 2025

First Submitted That Met QC Criteria

November 20, 2025

First Posted (Estimated)

December 1, 2025

Study Record Updates

Last Update Posted (Actual)

December 5, 2025

Last Update Submitted That Met QC Criteria

November 29, 2025

Last Verified

November 1, 2025

More Information

Terms related to this study

Other Study ID Numbers

  • IRB23082901

Plan for Individual participant data (IPD)

Plan to Share Individual Participant Data (IPD)?

NO

Drug and device information, study documents

Studies a U.S. FDA-regulated drug product

No

Studies a U.S. FDA-regulated device product

No

This information was retrieved directly from the website clinicaltrials.gov without any changes. If you have any requests to change, remove or update your study details, please contact register@clinicaltrials.gov. As soon as a change is implemented on clinicaltrials.gov, this will be updated automatically on our website as well.

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