Bioequivalence Study of GZR18 Injection Before and After CMC Change

A Randomized, Open-label, Single-dose, Parallel Comparison Study to Evaluate the Bioequivalence of GZR18 Injection Before and After CMC Change in Healthy Adult Male Subjects

This is an open-label, randomized, single-dose, parallel comparison bioequivalence study conducted in healthy adult male subjects.

The study is divided into 3 parts, each of which evaluates the bioequivalence of GZR18 Injection for one strength before and after CMC changes.

Subjects eligible for each part of the screening will be randomized into Group A or Group B in a 1:1 ratio. Each subject will receive a single dose of GZR18 Injection, followed by PK blood sampling and safety follow-up for 35 days. Subjects will be admitted to the hospital 1 day predose, follow a standardized light diet that evening, and then fast for at least 10 h thereafter. On the day of administration, GZR18 Injection of a single strength will be administered subcutaneously under fasting conditions at a site approximately two finger-widths (2.5 cm) away from the umbilicus on the abdomen. Lunch and dinner will be served approximately 3 and 9 h postdose, respectively.

Venous blood will be collected at 14 time points for GZR18 plasma concentration analysis: at 0 h (within 60 min predose) and 1, 6, 24, 48, 72, 96, 120, 168, 240, 336, 504, 672, and 840 h postdose.

Subjects will undergo corresponding laboratory safety tests at screening, D36, and early withdrawal visits.

During the entire study period, subjects should maintain a light diet, avoid strenuous exercise, and refrain from consuming juice, caffeinated beverages (such as tea and coffee), and alcoholic drinks. Smoking and any other activities that may affect PK observation or safety evaluations are strictly prohibited.

Study Overview

• Status: Recruiting
• Conditions: Obesity&T2D
• Intervention / Treatment: Drug: GZR18 injection A; Drug: GZR18 injection B

• Study Type: Interventional
• Enrollment (Estimated): 138
• Phase: Phase 1

Contacts and Locations

• Study Contact: Name: Xin Zhang Xin Zhang; Phone Number: 010-56456739; Email: xin5.zhang@ganlee.com

Study Locations

• China
  • Suzhou
    • Suzhou, Suzhou, China
      • Recruiting
      • The Second Affiliated Hospital of Soochow University
      • Contact: Xin Zhang Xin Zhang; Phone Number: 010-56456739; Email: xin5.zhang@ganlee.com

Participation Criteria

Eligibility Criteria

• Ages Eligible for Study: Adult
• Accepts Healthy Volunteers: Yes

Description

Inclusion Criteria:

1. Subjects who voluntarily sign the Informed Consent Form (ICF), can receive SC injection, fully understand the content, process and possible adverse reactions of the trial, and are able to follow the regulations on contraindications and restrictions specified in this protocol.
2. Male, 18 to 50 years of age at signing the ICF (both inclusive).
3. Weight ≥ 50 kg and body mass index (BMI) within 19-26 kg/m2 (inclusive) at screening.

4. Subjects of childbearing potential with no birth plan from the signing of ICF to 8 weeks after the last dose, willingness to take effective contraceptive measures, and no plan for sperm donation.

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Exclusion Criteria:

1. Subjects with clinically significant abnormalities detected during the screening period in physical examination, vital signs, laboratory tests, 12-lead ECG, abdominal ultrasound, or chest X-ray, as determined by the investigator.
2. Subjects who test positive for hepatitis B virus surface antigen, hepatitis C virus IgG antibody, human immunodeficiency virus antibody, P24 antigen, or anti-treponema pallidum antibody.
3. Subjects with a history of or existing circulatory, urinary, digestive, respiratory, nervous, endocrine, or psychiatric disorders that, in the investigator's judgment, remain clinically significant.
4. History of acute or chronic pancreatitis and pancreatic injury before screening.
5. History or family history of previous or existing medullary thyroid carcinoma, multiple endocrine neoplasia type 2.
6. Subjects who have used any drugs that alter the activity of drug metabolizing enzymes or transporters within 4 weeks prior to screening, or subjects with acute diseases or concomitant medication from the screening period to before randomization.
7. Subjects with severe infection or unexplained infection within 4 weeks before screening.
8. Major surgery within 6 months prior to screening, or scheduled surgery or hospitalization during the study.
9. Subjects known or suspected to have hypersensitivity to any ingredient of the investigational medicinal product (IMP) (glucagon like peptide-1 receptor agonist (GLP-1RA) or its excipients).
10. Use of any prescription drugs, over-the-counter drugs or Chinese herbal medicine within 2 weeks prior to screening; use of any GLP-1R agonists or drugs with the same mechanism of action to GLP-1R agonists (such as GLP-1R/glucagon receptor [GCGR] agonists or gastric inhibitory polypeptide receptor [GIPR]/GLP-1R agonists or GIPR/GLP-1R/GCGR agonists) within 3 months prior to screening.
11. Subjects who have been vaccinated within 1 month before screening or are scheduled for vaccination during the study.
12. Blood donation or blood loss greater than or equal to 400 mL within 3 months before screening, or blood donation scheduled during the study or within 8 weeks after the end of the study.
13. History of drug abuse prior to screening; or positive results for drug abuse at screening (D-1).
14. History of alcohol abuse within 3 months prior to screening, defined as an average intake of more than 14 units per week (1 standard unit=360 mL of beer or 150 mL of 12% wine or 45 mL of 40% spirits); or use of any alcohol-containing products 48 hours before administration; or abnormal results for breath alcohol test at screening (D-1).
15. Subjects who smoked more than 5 cigarettes per day on average within the 3 months before screening, or who do not agree to abstain from smoking for the duration of the study.
16. Subjects who engage in strenuous exercise within 48 h before administration of the IMP, or have other factors that may affect drug absorption, distribution, metabolism, or excretion.
17. Subjects who consume grapefruit or grapefruit-containing products, or any caffeinated or xanthine-containing foods or beverages (such as coffee, tea, cola, or chocolate) within 48 hours before administration of the IMP.
18. Subjects who have special dietary requirements and cannot adhere to a standardized diet, or who are lactose intolerant.
19. Subjects with a history of needle phobia and blood phobia, difficulty in blood collection or intolerance to venous blood collection.
20. Subjects who are investigators or employees of the study site, or family members of the employees or investigators.

21. Participation in a clinical study of another IMP or device within 3 months before screening, or within 5 half-lives of the previous IMP (whichever is longer). Or plan to participate in another clinical study of an IMP or device before completing all scheduled assessments in the clinical study.

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Study Plan

How is the study designed?

Design Details

• Primary Purpose: Other
• Allocation: Randomized
• Interventional Model: Parallel Assignment
• Masking: None (Open Label)

Number of Arms

6

Arms and Interventions

Participant Group / Arm	Intervention / Treatment
Experimental: GZR18 injection A1; Single dose, s.c.	Drug: GZR18 injection A; Administered SC
Experimental: GZR18 injection B1; Single dose, s.c.	Drug: GZR18 injection B; Single dose, s.c.
Experimental: GZR18 injection A2	Drug: GZR18 injection A; Administered SC
Experimental: GZR18 injection B2	Drug: GZR18 injection B; Single dose, s.c.
Experimental: GZR18 injection A3	Drug: GZR18 injection A; Administered SC
Experimental: GZR18 injection B3	Drug: GZR18 injection B; Single dose, s.c.

What is the study measuring?

Primary Outcome Measures

Outcome Measure	Time Frame
the maximum concentration (Cmax)	Day1-Day36
the area under the concentration-time curve during a dosing interval (AUC0-t)	Day1-Day36
the area under the concentration-time curve from the time of dosing extrapolated to infinity (AUC0-∞)	Day1-Day36

Secondary Outcome Measures

Outcome Measure	Time Frame
AUC extrapolated from the last time point extrapolated to infinity in percentage of the AUC0-∞ (AUC_%Extra)	Day1-Day36
time of maximum observed concentration (Tmax)	Day1-Day36
elimination half-life (t1/2)	Day1-Day36
elimination rate constant of plasma concentration at terminal phase (λz)	Day1-Day36
Treatment-Emergent Adverse Events (TEAEs)	Day1-Day36

Collaborators and Investigators

• Sponsor: Gan & Lee Pharmaceuticals.

Study record dates

Study Major Dates

• Study Start (Actual): January 9, 2026
• Primary Completion (Estimated): May 20, 2026
• Study Completion (Estimated): May 20, 2026

Study Registration Dates

• First Submitted: January 5, 2026
• First Submitted That Met QC Criteria: January 13, 2026
• First Posted (Actual): January 21, 2026

Study Record Updates

• Last Update Posted (Actual): January 22, 2026
• Last Update Submitted That Met QC Criteria: January 21, 2026
• Last Verified: January 1, 2026

More Information

Terms related to this study

• Other Study ID Numbers: GZR18-OOO-108

Drug and device information, study documents

• Studies a U.S. FDA-regulated drug product: No
• Studies a U.S. FDA-regulated device product: No