Phenotypic and Genotypic Detection of Colistin Resistance

March 17, 2026 updated by: Christen Saad Welliam, Assiut University

Phenotypic and Genotypic Detection of Colistin Resistance Among Multi Drug Resistant Gram-negative Bacteria in Assiut University Hospital

  • Evaluation of the disk elution test as reliable routine method for detection of colistin resistant among multidrug resistant gram-negative bacteria compared with broth microdilution.
  • Molecular detection of colistin resistant in multidrug-resistant gram-negative bacteria.

Study Overview

Status

Not yet recruiting

Conditions

Intervention / Treatment

Study Type

Observational

Enrollment (Estimated)

90

Contacts and Locations

This section provides the contact details for those conducting the study, and information on where this study is being conducted.

Study Contact

Participation Criteria

Researchers look for people who fit a certain description, called eligibility criteria. Some examples of these criteria are a person's general health condition or prior treatments.

Eligibility Criteria

Ages Eligible for Study

  • Child
  • Adult
  • Older Adult

Accepts Healthy Volunteers

No

Sampling Method

Probability Sample

Study Population

Multidrug-resistant gram-negative isolates will be collected from clinical specimens (blood, urine, sputum, wound swabs, etc.) obtained from hospitalized patients in Assiut University Hospitals.

Description

Inclusion Criteria:

-Multidrug-resistant gram-negative isolates will be collected from clinical specimens (blood, urine, sputum, wound swabs, etc.) obtained from hospitalized patients in Assiut University Hospitals.

Exclusion Criteria:

  • gram-positive bacteria
  • carbapenem-sensitive gram-negative bacteria
  • repeated sample for same patient or different samples from the same patient

Study Plan

This section provides details of the study plan, including how the study is designed and what the study is measuring.

How is the study designed?

Design Details

Cohorts and Interventions

Group / Cohort
Intervention / Treatment
multi drug resistant gram-negative bacteria in Assiut university hospital
Diagnostic test: disk elution test
  • Identification of microorganism using conventional methods or Vitek 2 compact system.
  • Anti biotic susceptibility test using conventional methods or Vitek 2 compact system.
  • For MDR gram negative bacteria: disc elution test and colistin broth microdilution

What is the study measuring?

Primary Outcome Measures

Outcome Measure
Time Frame
Evaluation of the disk elution test for detection of colistin resistant among multidrug resistant gram-negative bacteria compared with broth microdilution. And Molecular detection of colistin resistant in multidrug-resistant gram-negative bacteria by PCR
Time Frame: start from 2026 up to 2029
start from 2026 up to 2029

Collaborators and Investigators

This is where you will find people and organizations involved with this study.

Sponsor

Assiut University

Study record dates

These dates track the progress of study record and summary results submissions to ClinicalTrials.gov. Study records and reported results are reviewed by the National Library of Medicine (NLM) to make sure they meet specific quality control standards before being posted on the public website.

Study Major Dates

Study Start (Estimated)

August 1, 2026

Primary Completion (Estimated)

August 1, 2029

Study Completion (Estimated)

August 1, 2029

Study Registration Dates

First Submitted

March 14, 2026

First Submitted That Met QC Criteria

March 17, 2026

First Posted (Actual)

March 23, 2026

Study Record Updates

Last Update Posted (Actual)

March 23, 2026

Last Update Submitted That Met QC Criteria

March 17, 2026

Last Verified

March 1, 2026

More Information

Terms related to this study

Keywords

Other Study ID Numbers

  • Assiut organization

Plan for Individual participant data (IPD)

Plan to Share Individual Participant Data (IPD)?

UNDECIDED

Drug and device information, study documents

Studies a U.S. FDA-regulated drug product

No

Studies a U.S. FDA-regulated device product

No

This information was retrieved directly from the website clinicaltrials.gov without any changes. If you have any requests to change, remove or update your study details, please contact register@clinicaltrials.gov. As soon as a change is implemented on clinicaltrials.gov, this will be updated automatically on our website as well.

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