Effects of Storage on Lactate in Blood Samples

Effects of Storage on Plasma Lactate Determinations

Patrocinadores

Patrocinador principal: National Institute of Allergy and Infectious Diseases (NIAID)

Fuente National Institute of Allergy and Infectious Diseases (NIAID)
Resumen breve

The purpose of this study is to determine whether laboratory storage of samples affects the amount of lactate over 3 years.

Lactate is a natural substance normally present in the body. Lactate levels can go up for many different reasons, including treatment with nucleotide reverse transcriptase inhibitor drugs. This study will help researchers know if stored blood samples can be used to test lactate levels.

Descripción detallada

Lactic acidosis syndrome is a complication of therapy with nucleoside reverse transcriptase inhibitor (NRTI) drugs. Measurement of lactate levels is important in clinical trials of antiretroviral treatments. The preferred collection method for determining plasma lactate is with NaF/KOx tubes, while a number of ACTG studies have stored blood in ethylenediaminetetraacetic acid (EDTA) tubes. This study will analyze lactate in samples collected and stored in NaF/KOx and EDTA tubes to investigate the reliability of lactate concentrations over time.

Pre- and post-exercise blood samples are drawn from participants into NaF/KOx and EDTA tubes and a portion is tested for lactate levels immediately. The remaining portions are frozen and tested at 1, 3, 6, 12, 18, 24, and 36 months.

Estado general Completed
Fecha de inicio January 2001
Tipo de estudio Observational
Inscripción 13
Condición
Elegibilidad

Criterios:

Inclusion Criteria

Participants may be eligible for this study if they:

- Are at least 18 years old.

- Have sufficient forearm veins.

Exclusion Criteria

Participants will not be eligible for this study if they:

- Have a physical disability that prevents forearm exercise.

- Have any known medical reason, such as anemia, for not having 82 ml of blood drawn.

Género: All

Edad mínima: 18 Years

Edad máxima: N/A

Voluntarios Saludables: Accepts Healthy Volunteers

Oficial general
Ubicación
Instalaciones:
UCSF - San Francisco Gen Hosp | San Francisco, California, 94110, United States
Indiana Univ Hosp | Indianapolis, Indiana, 462025250, United States
Division of Inf Diseases/ Indiana Univ Hosp | Indianapolis, Indiana, 46202, United States
Ubicacion Paises

United States

Fecha de verificación

November 2004

Palabras clave
Tiene acceso ampliado No
Condición Examinar
Fuente: ClinicalTrials.gov