The Effect of Remote Ischemic Preconditioning Applied in Children the Day Before Open Heart Surgery

실험적: Preconditioning

Children who received the preconditioning stimulus

절차: Remote ischemic preconditioning

Remote ischemic preconditioning was induced by four 5-minutes cycles of lower limb ischemia and reperfusion using a blood pressure cuff

다른 이름들:

• Inicial letters of the full name
• Register number provided by hospital

간섭 없음: Control

Children who did not receive the preconditioning stimulus

IkB-alpha Expression

Expressure of gene of an inhibitory protein called kappa-B alpha (IkB-alpha). Inhibits the inflammatory response protein called kappa-B nuclear factor. To measure that expression we used a real time protein chain reaction (RT-PCR), always comparing with an endogenous protein expression (this way, the encountered value is apresented in "arbitraries units", that means how much times the expression of the protein IkB-alpha is bigger than the endogenous protein that present a invariable value.

Interleucine 8

Quantification of interlecine 8 (a pro-inflammatory protein) using the ELISA method

NT-proBNP

Plasma concentration of the amino-terminal of B-type natriuretic peptite (NT-proBNP)was measured by enzyme electrochemiluminescence immunoassay.

Troponin I

Seric concentration of troponin I, a myocardial cell injury marker. We measured by solid-phase chemiluminescence immunoassay.