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- Ensaio Clínico NCT04659850
Impact of AGEs on Metabolism & Cognition (AGEs)
Do Dietary AGEs Have an Acute Effect on Metabolism and Cognition
Visão geral do estudo
Status
Condições
Descrição detalhada
This study will be a short-term, randomized, double-blind, cross-over trial. Volunteers will be younger (18-30 years) and older (55-75 years) men and women, normal and overweight (BMI>19 to 29.9). N=24 in each age group.
Medical screening for all study volunteers and neuropsychological screening will be carried out prior to study start. A small, pin prick, fasted blood sample will be taken to measure glucose and HbA1C levels to rule out type 2 diabetics. Anthropometric and body composition measurements will also be taken. Body weight and height will be measured using a scale and a stadiometer to the nearest 0.5 kg and 0.5 cm, respectively. Waist and hip circumferences will be measured and the waist-to-hip ratio calculated. Standard procedures will be followed for the anthropometric measurements and the average of three measurements taken. Neuropsychological screening may be repeated on another day if volunteers are borderline on their first visit as scores may vary by a couple of points depending on external events i.e. vounteers are having a bad day. Experienced staff will carry out abdominal fat measurements by manual determination of the area between the upper surface of the second lumbar vertebra, the lower surface of the fourth lumbar vertebra, and the lateral margins of the outer rib cage. If the Viscan is available we will use that to measure abdominal fat. Venous blood samples will be collected as appropriate for the hormones and metabolites to be tested and stored at -70 °C.
The day prior to the challenge volunteers will visit the Human Nutrition Unit (HNU, RINH). They will be given a low AGE breakfast and be supplied with a low AGE lunch and dinner for that day and instructed not to consume anything else. After an overnight fast volunteers will receive a one-off dose of either high AGE (11.4 mgs) or low AGE (2.4 mgs) whey protein delivered in a either a low or high fat milkshake (400ml) blended with flavours and a consistent quantity of sugar. All ingredients will be commercially available and of food grade. Apart from differences in fat and AGE the content of shakes will be closely balanced for sugar and other components. All participants will be asked to answer two questionnaires one to determine their habitual intake of dietary AGEs and one to determine their level of physical activity.
The different milkshakes will be tested with a minimum 7 day wash out period between tests. Volunteers will be randomised to each test group. After a 12 h overnight fast and a standardised intake the day prior to the tests, and no strenuous physical activity for 2 days previously, subjects will attend the HNU. Volunteers will be requested to provide a small urine sample for AGE analysis. An intravenous catheter will then be placed in an antecubital vein and flushed with saline between aspirations to keep the catheter clear. After a short resting period, a blood sample will be taken prior to the first memory test then a single milkshake will be consumed under supervision. Blood samples will be taken immediately before the milkshake ingestion (0 min) and after 30, 60, 90, 120, 150 and 180 and 240 min. Further computer based memory tests will be carried out at 30, 90 and 240 minutes. A urine sample will be requested at the end of the study before participants are offered lunch. Memory tests have been designed by Dr Ros Langston (University of Dundee) and Dr Kevin Allan, School of Psychology and will be carried out on lap tops specifically designated for this study. Blood and urine will be analysed for AGEs. Blood will also be analysed for glucose lipids and hormones. Dr Janice Drew will also analyse blood samples for gene expression of the SIRT-1/NAD+ complex indicative of the response of individuals to dietary challenge.
Tipo de estudo
Inscrição (Antecipado)
Estágio
- Não aplicável
Contactos e Locais
Locais de estudo
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Scotland
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Aberdeen, Scotland, Reino Unido, AB252ZD
- Recrutamento
- University of Aberdeen Rowett Institute of Nutrition and Health
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Contato:
- Fiona Campbell, PHD
- Número de telefone: 01224438617
- E-mail: fiona.campbell@abdn.ac.uk
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Contato:
- Christine Grant, HNC Biology
- Número de telefone: 01224438708
- E-mail: christine.grant@abdn.ac.uk
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Critérios de participação
Critérios de elegibilidade
Idades elegíveis para estudo
Aceita Voluntários Saudáveis
Gêneros Elegíveis para o Estudo
Descrição
Inclusion Criteria:
- Heathy
- Sedentary
- 18-30 or
- 55-75
Exclusion Criteria:
- diagnosed type 2 diabetes,
- CVD
- history of neurological abnormalities
- current psycho-active medication
- current anti-inflammatory medication
- Current glucose lowering medication
Plano de estudo
Como o estudo é projetado?
Detalhes do projeto
- Finalidade Principal: Prevenção
- Alocação: Randomizado
- Modelo Intervencional: Atribuição cruzada
- Mascaramento: Dobro
Armas e Intervenções
Grupo de Participantes / Braço |
Intervenção / Tratamento |
---|---|
Outro: Group 1
Group 1 participants in the young 18 - 30 age group
|
low fat, low AGE milkshake given after an overnight fast
low fat, high AGE milkshake given after an overnight fast
high fat, low AGE milkshake given after an overnight fast
high fat, high AGE milkshake given after an overnight fast
|
Outro: Group 2
Group 2 participants in the older 55 - 75 age group
|
low fat, low AGE milkshake given after an overnight fast
low fat, high AGE milkshake given after an overnight fast
high fat, low AGE milkshake given after an overnight fast
high fat, high AGE milkshake given after an overnight fast
|
O que o estudo está medindo?
Medidas de resultados primários
Medida de resultado |
Descrição da medida |
Prazo |
---|---|---|
Impact of Advanced Glycation End Products on Metabolism and Cognition
Prazo: Four weeks per participant
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Habitual AGE intake, determined from dietary questionnaires, specifically designed for this purpose, taken at the start of the study, will be correlated with levels of circulating AGEs and outcomes such as inflammatory markers and expression of genes associated with susceptibility to metabolic disease and acute response to dietary challenge will be measured.
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Four weeks per participant
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Colaboradores e Investigadores
Patrocinador
Colaboradores
Investigadores
- Investigador principal: Fiona Campbell, PHD, UoA Rowett Institute of Nutrition and Health
Datas de registro do estudo
Datas Principais do Estudo
Início do estudo (Real)
Conclusão Primária (Antecipado)
Conclusão do estudo (Antecipado)
Datas de inscrição no estudo
Enviado pela primeira vez
Enviado pela primeira vez que atendeu aos critérios de CQ
Primeira postagem (Real)
Atualizações de registro de estudo
Última Atualização Postada (Real)
Última atualização enviada que atendeu aos critérios de controle de qualidade
Última verificação
Mais Informações
Termos relacionados a este estudo
Outros números de identificação do estudo
- 2017/RI/5
Informações sobre medicamentos e dispositivos, documentos de estudo
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Essas informações foram obtidas diretamente do site clinicaltrials.gov sem nenhuma alteração. Se você tiver alguma solicitação para alterar, remover ou atualizar os detalhes do seu estudo, entre em contato com register@clinicaltrials.gov. Assim que uma alteração for implementada em clinicaltrials.gov, ela também será atualizada automaticamente em nosso site .
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