Dose Ranging Study of ART621 in Subjects Diagnosed With Rheumatoid Arthritis Taking Methotrexate

Multi-Centre Randomised Double-Blind Pbo-Controlled Dose-Ranging Study to Evaluate the Safety, Tolerability, Efficacy, PK and Immunogenicity of Multiple Doses of ART621 for 3 Months in Patients With Rheumatoid Arthritis Taking Methotrexate

The purpose of this clinical trial is to assess the safety, efficacy, tolerability, immunogenicity and pharmacokinetics of 3 dose levels of ART621 in the treatment of rheumatoid arthritis.

Study Overview

• Status: Terminated
• Conditions: Rheumatoid Arthritis
• Intervention / Treatment: Drug: ART621; Drug: ART621; Drug: ART621; Drug: Placebo

Detailed Description

Despite being effective in approximately 60% of subjects, there are limitations to existing anti-TNF therapies especially in relation to immunogenicity, safety and administration. In addition, due to their high molecular weight, currently marketed products are largely confined to the blood stream.

ART621 is an anti-TNF molecule that contains 2 identical domain "antibodies" that have the binding activity of a full antibody but with a substantially smaller molecular size. The molecular weight of approximately half that of full size antibodies is predicted to, a) have improved tissue penetration and, b) to be less immunogenic than full size antibodies.

This clinical trial is designed to assess the safety, efficacy, tolerability, immunogenicity and pharmacokinetics of ART621 when administered with an intravenous loading dose followed by subcutaneous administration every week.

• Study Type: Interventional
• Enrollment (Actual): 13
• Phase: Phase 2

Contacts and Locations

Study Locations

• Argentina
  • Calle French, Argentina, 2673
    • IMAI Research - Instituto Medico de Asistencia y Investigaci
  • San Miguel de Tucuman, Argentina
    • Centro Medico Privado de Reumatologia
  • BUE
    • Buenos Aires, BUE, Argentina, C1405BCH
      • Instituto Medico Especializado IME
  • Bue
    • Quilmes, Bue, Argentina, B1878DVB
      • Instituto Medico CER
  • CRD
    • Cordoba, CRD, Argentina, X5000BNB
      • ILAIM-CEOM Inst. Latinoamericano de Inv. Medicas
  • SFE
    • Rosario, SFE, Argentina, 2000
      • Sanatorio Parque S.A.
    • Rosario, SFE, Argentina, S2000PBJ
      • CAICI
  • SJN
    • San Juan, SJN, Argentina, 5400
      • Ctro Polivalente de Asist e Invest Clinica CER
  • TUC
    • San Miguel de Tucuman, TUC, Argentina, T4000AXL
      • Centro Medico Privado de Reumatologia
    • Tucuman, TUC, Argentina, 4000
      • Centro de Investigaciones Reumatológicas
• Australia
  • New South Wales
    • Elizabeth Vale, New South Wales, Australia, 5112
      • Lyell McEwin Hospital
    • Sydney, New South Wales, Australia, 2298
      • Georgetown Arthritis Centre
  • Queensland
    • Maroochydore, Queensland, Australia, 4558
      • Coast Joint Care
  • South Australia
    • Woodville, South Australia, Australia, 5011
      • The Queen Elizabeth Hospital
• Czech Republic
  • Bruntal, Czech Republic, 79201
    • Revmatologicka ambulance
  • Jihlava, Czech Republic, 58633
    • Nemocnice Jihlava
  • Pardubice, Czech Republic, 53002
    • ARTHROMED s. r. o.
  • Plzen - Bory, Czech Republic, 30599
    • Fakultni nemocnice Plzen
  • Praha 2, Czech Republic, 12850
    • Revmatologicky ustav
• India
  • Mangalore, India, 575001
    • K. M. C. Hospital
  • Andh Prad
    • Hyderabaad, Andh Prad, India, 500033
      • Apollo Hospital Educational and Research Foundation
    • Hyderabaad, Andh Prad, India, 500082
      • Nizams Institute of Medical Sciences
    • Secunderabad, Andh Prad, India, 500003
      • Krishna Institute of Medical Sciences
    • Vishakhapattanam, Andh Prad, India, 530002
      • King George Hospital
  • Karna
    • Bangalore, Karna, India, 560034
      • St. John's Medical College Hospital
    • Bangalore, Karna, India, 560054
      • M.s. Ramaiah Memorial Hospital
  • Mahara
    • Pune, Mahara, India, 411001
      • Centre for Rheumatic Diseases
  • Tamilnadu
    • Madurai, Tamilnadu, India, 625020
      • Apollo Hospitals Educational and Research Foundation
  • Uttar Prad
    • Lucknow, Uttar Prad, India, 226014
      • Sanjay Gandhi Postgraduate Institute
• Malaysia
  • Kuala Lumpur, Malaysia, 50480
    • University Malaya Medical Centre
  • Perak, Malaysia, 30990
    • Hospital Ipoh
  • Sarawak, Malaysia, 93586
    • Sarawak General Hospital
  • Kedah
    • Alor Setar, Kedah, Malaysia, 05100
      • Putra Medical Centre
• New Zealand
  • Christchurch, New Zealand, 8002
    • C G M Research Trust, The Princess Margaret Hospital
• Poland
  • Bialystok, Poland, 15-461
    • NZOZ Centrum Osteoporozy i Chorob Kostno-Stawowych
  • Dzialdowo, Poland, 13-200
    • Samodzielny Publiczny ZOZ w Dzialdowie
  • Lublin, Poland, 20-607
    • NZOZ Reumed
  • Poznan, Poland, 60-218
    • Medyczne Centrum Hetmańska
  • Szczecin, Poland, 71-252
    • SPSK nr 1 im. Tadeusza Sokolowskiego PAM
  • Warszawa, Poland, 02-341
    • Centrum Medyczne Osteomed
• United States
  • Florida
    • Tampa, Florida, United States, 33614
      • Tampa Medical Group P.A.
  • Illinois
    • Springfield, Illinois, United States, 62704
      • Arthritis Center
  • Nebraska
    • Lincoln, Nebraska, United States, 68516
      • Physician Research Collaboration, LLC
    • Omaha, Nebraska, United States, 68114
      • Westroads Medical Group
  • Washington
    • Spokane, Washington, United States, 99204
      • Arthritis Northwest, PLLC
  • Wisconsin
    • Racine, Wisconsin, United States, 53142
      • Arthritis Clinic

Participation Criteria

Eligibility Criteria

• Ages Eligible for Study: 18 years to 80 years (Adult, Older Adult)
• Accepts Healthy Volunteers: No
• Genders Eligible for Study: All

Description

Inclusion Criteria:

• Provision of a valid written informed consent.
• Male or female subjects ≥ 18 and ≤ 80 years old.
• Women of childbearing potential, or men of fathering potential, must be using adequate (in the investigator's opinion) birth control measures (e.g. abstinence, oral contraceptives, intrauterine device, barrier method with spermicide, or surgical sterilisation) during the study. Female subjects of childbearing potential must test negative for pregnancy prior to enrolling in the study. Post menopausal (cessation of menses for more than 2 years) women are eligible for this study.
• Diagnosis of RA according to the revised (1987) American College of Rheumatology criteria for at least 6 months and no longer than 3 years prior to screening.
• Meet ACR functional class criteria I, II or III.

• Have active RA at the time of screening and at baseline, defined as ≥ 6 swollen joints and ≥ 6 tender joints (from 68 joint count) together with at least 2 of the following 3 criteria:

  • CRP level ≥ 1.5 mg/dl;
  • ESR by Westergren method ≥ 28 mm in the first hour; or
  • morning stiffness ≥ 45 minutes.

• At least one of the following should be present at screening:

  • documented history or current presence of positive rheumatoid factor;
  • presence of serum anti-CCP antibodies; or
  • screening radiographic erosion
• Have been tolerating concomitant methotrexate (oral or subcutaneous) for at least 3 months prior to screening and on a stable dose between 10-25 mg per week for at least 6 weeks prior to the first study dose. The route of administration must also be stable. Use of methotrexate dose of 25-50 mg every 2 weeks is also acceptable. (Other DMARDs taken concomitantly with methotrexate are not allowed. Those subjects concomitantly receiving additional DMARDs with methotrexate may enter the study by stopping the additional DMARD at least 4 weeks prior to first study dose).

• If using the following medication, the subject must be on a stable dose for the 4 weeks prior to the first study dose and maintain that dose throughout the study:

  • oral corticosteroids, equivalent to ≤ 10 mg of prednisone/day.
  • one nonsteroidal anti-inflammatory drug (NSAID).
  • 3-hydroxy-3-methylglutaryl-coenzyme A reductase inhibitors (statins) or fibrates (see Section 7.1 for acceptable doses).
• Does not have active or latent TB according to eligibility assessment and screening rules (see Section 8.3.1).
• Is willing and able to comply with study visits and other protocol requirements.

Exclusion Criteria:

• Pregnant, nursing, or planning a pregnancy (both men and women) within 9 months of enrolment.
• Subjects weighing > 100 kgs.

• Screening laboratory tests:

  • hemoglobin ≤ 8.0 gm/dl
  • white blood cells ≤ 3.0 x103 cells/µl
  • neutrophils ≤ 1.5 x 103 cells/µl
  • platelets ≤100 x 103 cells/µl
  • serum transaminase level (AST and ALT) ≥ 2 times upper limit of normal (ULN)
  • serum creatinine ≥ 0.15 mmol/l
• Subjects with diagnosis of juvenile arthritis or other inflammatory or autoimmune diseases that might confound the evaluations of benefit from ART621 such as ankylosing spondylitis, systemic lupus erythematosus and Lyme disease.
• Subjects who have previously failed to respond to any oral or injectable anti-TNFα therapy or subjects who have had to stop anti-TNFα therapy for safety reasons. Subjects who have successfully responded to anti-TNFα therapy in the past (but discontinued for reasons other than safety or lack of efficacy) > 6 months prior to study day one may enrol. Patients who have participated in a previous anti-TNFα therapy study are eligible if they are confirmed to have received placebo.
• Subjects who have previously received the following anti-rheumatic drugs: interleukin-1 receptor antagonist [anakinra], rituximab, anti-CD4 antibody, abatacept, thalidomide, p38 MAP kinase inhibitor and other agents (other than those listed in Section 7.3).
• Subjects who have undergone plasmapheresis within 6 months prior to randomisation.
• Have received intraarticular, intramuscular, or intravenous corticosteroids, including intramuscular adrenocorticotropic hormone, during the 4 weeks prior to the first study dose, or non-stable doses of oral steroids.
• Subjects with a history of any clinically significant adverse reaction to murine or chimeric proteins, including serious allergic reactions.
• Subjects with Felty's syndrome or a history of Felty's syndrome.
• Subjects who have received or are expecting to receive any live virus or bacterial vaccinations within 1 month before first study dose, during the study, or up to 3 months after the study dose.

• Subjects with a history of, presence of, or at high risk of serious infection including:

  • history of active TB, or positive Mantoux test or QuantiFERON Gold test or chest x-ray suggestive of active or healed TB or positive contact history with a subject with active TB within the past 3 months. If subjects have a positive Mantoux test but a negative QuantiFERON Gold test, they may be enrolled.
  • a serious infection during the 3 months prior study entry (hospitalised or received IV antibiotics for an infection).
  • chronic or recurrent infectious disease.
  • systemic fungal infections
  • opportunistic infection within 3 months prior to screening (refer to 1993 CDC Classification System for HIV Infection).
  • subjects known, or suspected, to be infected with HIV, hepatitis B, or hepatitis C.
  • subjects with planned joint replacement surgery or a history of infected joint prosthesis or who have received antibiotics for a suspected infection of a joint prosthesis if that prosthesis has not been removed or replaced.
• Subjects with a known history of demyelinating diseases such as multiple sclerosis or optic neuritis.
• Subjects with evidence of severe, progressive, or uncontrolled renal, hepatic, hematologic, gastrointestinal, endocrine, pulmonary, cardiac, neurologic, psychiatric, or cerebral disease.
• Concurrent CHF, including medically controlled, asymptomatic CHF or ECG findings suggestive of CHF.
• Subjects receiving cytotoxic drugs including cyclophosphamide, cyclosporine, or alkylating agents within 6 months prior to first study dose.
• Known history or evidence of malignancy, lymphoproliferative or neoplastic disease with the exception of successfully treated basal or squamous cell carcinoma of the skin or cervical intraepithelial neoplasia.
• Subjects who have undergone organ transplant (with exception of a corneal transplant more than 3 months prior to screening).
• Subjects previously enrolled in this study, currently participating in another investigational study or treated with any investigational drug within the previous 3 months or within 5 half-lives, whichever is greater, prior to first study dose.
• Any other clinically significant disease or disorder or factors such as substance abuse which in the opinion of the investigator make the subject ineligible for participation in this study.

Study Plan

How is the study designed?

Design Details

• Primary Purpose: Treatment
• Allocation: Randomized
• Interventional Model: Parallel Assignment
• Masking: Double

Number of Arms

4

Arms and Interventions

Participant Group / Arm	Intervention / Treatment
Placebo Comparator: Placebo arm	Drug: Placebo; Placebo s.c.
Experimental: ART621 A; ART621 0.75mg/kg per week	Drug: ART621; 3.0mg/kg s.c.; Drug: ART621; 1.5mg/kg s.c.; Drug: ART621; 0.75mg/kg s.c.
Experimental: ART621 B; ART621 1.5 mg/kg per week	Drug: ART621; 3.0mg/kg s.c.; Drug: ART621; 1.5mg/kg s.c.; Drug: ART621; 0.75mg/kg s.c.
Experimental: ART621 C; ART621 3.0mg/kg per week	Drug: ART621; 3.0mg/kg s.c.; Drug: ART621; 1.5mg/kg s.c.; Drug: ART621; 0.75mg/kg s.c.

What is the study measuring?

Primary Outcome Measures

Outcome Measure	Time Frame
Efficacy of ART621 on the signs and symptoms of moderate to severe RA in subjects concomitantly taking methotrexate as assessed by the proportion of subjects achieving an ACR20 response.	12 weeks

Secondary Outcome Measures

Outcome Measure	Time Frame
Dose-response relationship of ART621 against the signs and symptoms of moderate to severe RA as assessed by additional efficacy, safety and QoL parameters.	12 weeks
Immunogenicity profile of ART621 as assessed by development of HAHAs.	16 weeks
Plasma concentration versus time profile and population PK of ART621 in subjects with RA.	16 weeks
Effect of ART621 on immunological parameters and other disease biomarkers.	12 weeks

Collaborators and Investigators

• Sponsor: Arana Therapeutics Ltd

Study record dates

Study Major Dates

• Study Start: April 1, 2009
• Primary Completion (Actual): November 1, 2009
• Study Completion (Actual): December 1, 2009

Study Registration Dates

• First Submitted: June 24, 2009
• First Submitted That Met QC Criteria: June 24, 2009
• First Posted (Estimate): June 25, 2009

Study Record Updates

• Last Update Posted (Estimate): January 6, 2010
• Last Update Submitted That Met QC Criteria: January 4, 2010
• Last Verified: January 1, 2010

More Information

Terms related to this study

• Additional Relevant MeSH Terms: Immune System Diseases; Autoimmune Diseases; Joint Diseases; Musculoskeletal Diseases; Rheumatic Diseases; Connective Tissue Diseases; Arthritis; Arthritis, Rheumatoid
• Other Study ID Numbers: ART621-221