Characterization of the Effect of Tocilizumab in Vivo and in Vitro on T Follicular Helper Cells in Rheumatoid Arthritis Patients and Consequence on B Cells Maturation (Tocihelper)

Naïve CD4+ helper T (Th) cells, upon encountering their cognate antigens presented on professional antigen-presenting cells, differentiate into different effector cells:

• Th1 cells produce Interferon-γ and regulate antigen presentation and immunity against intracellular pathogens;
• Th2 cells produce IL-4 (Interleukin-4), IL-5 and IL-13, and mediate humoral responses and immunity against parasites;
• Th17 cells produce IL-17, IL-17F and IL-22 and regulate inflammatory responses by tissue cells.

An additional TH subset called follicular helper T (Tfh) cells has recently been identified in germinal centers and also in whole blood (circulating Tfh cells). These cells regulate B-cell maturation and immunoglobulin production during normal immune responses. They produce factors essential for B cell selection and maturation into memory B-cells or long-lived antibody-secreting plasma cells. Furthermore, they also seem to favor pathogenic autoantibody production in systemic autoimmunity, and therefore could potentially represent a novel therapeutic target in autoimmune diseases. Indeed, rheumatoid arthritis synovium is characterized by the presence of ectopic lymphoid structures, resembling germinal centers. Potentially, Tfh cells from these nonlymphoid tissues could promote B-cell maturation and synthesis of pathogenic autoantibody production, thus potentiating tissue injury. Interestingly, production of IL-21 by Tfh cells is implicated in B cell activation, and the same cytokine have been associated with rheumatoid arthritis (RA) pathogenesis.

IL-6 blocking therapy significantly reduces signs and symptoms as well as radiological progression in RA. However, so far, it has not been determined which of the pleiotropic IL-6 effects impact the observed clinical response. Recently, Samson et al have demonstrated that Tocilizumab (TCZ) corrects the imbalance between Th17 cells and Treg cells in patients with RA. More interestingly, the group of Hans-Peter Tony has reported the impact of TCZ on B cell compartment. They showed a significant reduction in the frequency of peripheral pre-switch and post-switch memory B cells but also a reduction of serum immunoglobulin levels, that could be the reflect of TCZ on Tfh cells development, circulation and/or function.

Most of the work studying the role of IL-6 on Tfh cells development has been performed in mice. They showed that optimal Tfh cells formation requires IL-21 and IL-6, and that cytokines alone are insufficient to drive Tfh cells differentiation.

To better understand the impact of IL-6 on human Tfh cells, the investigators would like to conduct a prospective study in patients with active RA and investigated the effects of blocking IL-6 with TCZ on circulating Tfh cells levels and Tfh cells subsets over a 12-week study period. Furthermore, the impact of TCZ treatment on Tfh cells generation will be explored in vitro.

Study Overview

• Status: Completed
• Conditions: Arthritis, Rheumatoid; Tocilizumab; T-Lymphocytes, Helper-Inducer

• Study Type: Observational
• Enrollment (Actual): 60

Contacts and Locations

Study Locations

• France
  • Aquitaine
    • Bordeaux, Aquitaine, France, 33076
      • Rheumatology department - Bordeaux University Hospital

Participation Criteria

Eligibility Criteria

• Ages Eligible for Study: 18 years and older (Adult, Older Adult)
• Accepts Healthy Volunteers: No
• Genders Eligible for Study: All

• Sampling Method: Non-Probability Sample

Study Population

This study will be performed as a prospective observational open-labeled study (non-interventional) to evaluate the impact of TCZ treatment on the evolution of circulating Tfh cells levels. The administration of treatments (TCZ and MTX) will be done according to current practice, and will not be motivated by the inclusion in this study. TCZ will be administered in association with a conventional DMARD, or in monotherapy, according to clinical recommendations. The objective of this study is to analyze the impact of new therapy set-up, and patients included in the TCZ group would be under MTX treatment for at least 3 months. The immunological analysis will be performed with drop-off of blood samples used in the recommended follow-up of patients treated by MTX or TCZ. For the healthy controls, the immunological analysis will be performed with drop-off of blood samples used in the recommended follow-up of patients treated for osteoporosis or osteoarthritis.

Description

Inclusion Criteria:

Inclusion in TCZ and MTX groups:

• Patients with active moderate to severe RA fulfilling ACR criteria and requiring TCZ or MTX treatment, according to the EU label and French authority recommendations, who accept to enter the study and to sign the informed consent.
• Patients > 18 years
• When treated with corticosteroids: Dose ≤10mg/jour within 4 weeks before inclusion and dose stable during the study.
• When treated with MTX (0.3 mg/kg/w): Dose stable within 4 weeks before inclusion and during the study to avoid confusion on Tfh levels evaluation.
• When treated by prior anti-TNF: according to the recommendations of the French "Club Rhumatismes Inflammatoires", a wash-out period of 5 half-lives will be defined to avoid confusion in the evaluation of Tfh cells initial levels.
• These patients will be consecutively included in the study until the number of needed patients will be reached.

Inclusion in the healthy controls group:

• Healthy controls will be defined as people non-affected by an inflammatory disease (such as RA, ankylosing spondylitis, lupus…). This group will be constituted with patients affected by sciatica, osteoarthritis, osteoporosis…
• These patients will be included in a second phase in order to guarantee the quality of matching with cases.

Exclusion Criteria:

• Patients previously treated with TCZ
• Patients with a history of allergic reactions or Hypersensitivity to TCZor to any of the excipients
• Patients with severe and uncontrolled infections such as sepsis and opportunistic infections
• Patients with hepatitis B (with virus replication) or C, HIV-infection and tuberculosis
• Patients with history of cancer or lymphoma
• Patients with history of diabetes

• Patients who presented one of the following laboratory abnormalities:

  • Serum creatinine > 1.6 mg/dL (141 μmol/L) in female patients and > 1.9 mg/dL (168 μmol/L) in male patients, only for MTX treated patients.
  • Alanine aminotransferase (ALT) or aspartate aminotransferase (AST) > 1.5 times upper limit of normal (ULN).
  • Platelet count < 100,000/mm3, (100 x 109/L).
  • Hemoglobin < 8.0 g/dL, (5.0 mmol/L).
  • White Blood Cells < 3000/mm3, (3.0 x 109/L).
  • Absolute neutrophil count < 2000/mm3, (2.0 x 109/L).
  • Absolute lymphocyte Count < 500/mm3, (0.5 x 109/L).
  • Positive hepatitis BsAg or hepatitis C antibody
  • Total bilirubin > ULN
• Pregnant or breast-feeding patients
• Patients who refuse to sign the informed consent

Study Plan

How is the study designed?

Design Details

• Observational Models: Cohort
• Time Perspectives: Prospective

Number of groups / cohorts

3

Cohorts and Interventions

Group / Cohort
Tocilizumab; Patients with active moderate to severe RA fulfilling ACR criteria and requiring TCZ treatment, according to the EU label and French authority recommendations, who accept to enter the study and to sign the informed consent.
Methotrexate; Patients with active moderate to severe RA fulfilling ACR criteria and requiring MTX treatment, according to the EU label and French authority recommendations, who accept to enter the study and to sign the informed consent.
Healthy Controls; Healthy controls will be defined as people non-affected by an inflammatory disease (such as RA, ankylosing spondylitis, lupus…). This group will be constituted with patients affected by sciatica, osteoarthritis, osteoporosis…

What is the study measuring?

Primary Outcome Measures

Outcome Measure	Time Frame
Change from Baseline in levels of circulating Tfh cells at 12 weeks for TCZ treated patients	12 weeks

Collaborators and Investigators

• Sponsor: Association Aquitaine de Recherche Clinique en Rhumatologie
• Collaborators: Roche Pharma AG; Chugai Pharma France

Study record dates

Study Major Dates

• Study Start: September 1, 2014
• Primary Completion (Actual): September 1, 2016
• Study Completion (Actual): December 1, 2016

Study Registration Dates

• First Submitted: October 6, 2015
• First Submitted That Met QC Criteria: October 6, 2015
• First Posted (Estimate): October 7, 2015

Study Record Updates

• Last Update Posted (Actual): January 9, 2019
• Last Update Submitted That Met QC Criteria: January 8, 2019
• Last Verified: January 1, 2019

More Information

Terms related to this study

• Additional Relevant MeSH Terms: Immune System Diseases; Autoimmune Diseases; Joint Diseases; Musculoskeletal Diseases; Rheumatic Diseases; Connective Tissue Diseases; Arthritis; Arthritis, Rheumatoid
• Other Study ID Numbers: ML29237