Effect of NO Precursors on Physiological Responses to Exercise (NO-SUP)

December 19, 2025 updated by: University Hospital, Grenoble

The Effect of NO Precursors on Physiological Responses to Exercise

NO is an ubiquitous molecule contributing to many physiological functions such as vasodilation, immune and oxidative stress responses. NO production can be supplemented either by providing substrate to the NO-synthase (L-Arginine and L-Citruline) or by providing nitrate which can lead to NO. In the present project the investigators evaluate the effect of various acute and chronic oral NO precursors supplementation on exercise performance and responses in healthy young and older subjects.

Study Overview

Status

Completed

Conditions

Intervention / Treatment

Study Type

Interventional

Enrollment (Actual)

63

Phase

  • Not Applicable

Contacts and Locations

This section provides the contact details for those conducting the study, and information on where this study is being conducted.

Study Locations

  • France
    • Auvergne-Rhône-Alpes
      • Grenoble, Auvergne-Rhône-Alpes, France, 38043
        • Grenoble University Hospital

Participation Criteria

Researchers look for people who fit a certain description, called eligibility criteria. Some examples of these criteria are a person's general health condition or prior treatments.

Eligibility Criteria

Ages Eligible for Study

18 years to 70 years (Adult, Older Adult)

Accepts Healthy Volunteers

Yes

Description

Inclusion Criteria:

  • Sedentary subjects
  • No cardiovascular, respiratory, metabolic and neurological disorders

Exclusion Criteria:

  • Body mass index > 28 kg.m-2
  • Smoker
  • Alcool intake > 10g/day
  • Psychiatric diseases
  • Subjects under treatment able to interfere with their cardiovascular, metabolic and neurological status

Study Plan

This section provides details of the study plan, including how the study is designed and what the study is measuring.

How is the study designed?

Design Details

  • Primary Purpose: Basic Science
  • Allocation: Randomized
  • Interventional Model: Parallel Assignment
  • Masking: Triple

Arms and Interventions

Participant Group / Arm
Intervention / Treatment
Active Comparator: Acute intake
Evaluation after acute intake of the food supplement.
Dietary supplement: Nitrate
Subjects will be supplemented orally either acutely or chronically (4-8 weeks) with NO precursors.
Dietary supplement: Nitrate and L-Arginine
Subjects will be supplemented orally either acutely or chronically (4-8 weeks) with NO precursors.
Dietary supplement: Nitrate and L-Citrulline
Subjects will be supplemented orally either acutely or chronically (4-8 weeks) with NO precursors.
Dietary supplement: Placebo
Subjects will be supplemented orally either acutely or chronically (4-8 weeks) with a placebo.
Biological: Evaluation of post-ischemic vascular reactivity
After the intake of the supplement. Concentration of hemoglobin (g/mL) in the quadriceps with the device : NIRS system Oxymon, ARTINIS.
Biological: Neuromuscular evaluations
After the intake of the supplement. Measure the stretching force in the ankle (N)
Active Comparator: Chronic intake
Evaluation before and after chronic intake of the food supplement.
Dietary supplement: Nitrate and L-Citrulline
Subjects will be supplemented orally either acutely or chronically (4-8 weeks) with NO precursors.
Dietary supplement: Placebo
Subjects will be supplemented orally either acutely or chronically (4-8 weeks) with a placebo.
Biological: Evaluation of post-ischemic vascular reactivity
After the intake of the supplement. Concentration of hemoglobin (g/mL) in the quadriceps with the device : NIRS system Oxymon, ARTINIS.
Biological: Neuromuscular evaluations
After the intake of the supplement. Measure the stretching force in the ankle (N)

What is the study measuring?

Primary Outcome Measures

Outcome Measure
Measure Description
Time Frame
Neuromuscular evaluations
Time Frame: 3 half days
After the intake of the supplement. Measure the stretching force in the ankle (N)
3 half days
Evaluation of post-ischemic vascular reactivity
Time Frame: 3 half days
After the intake of the supplement. Concentration of hemoglobin (g/mL) in the quadriceps with the device : NIRS system Oxymon, ARTINIS.
3 half days

Collaborators and Investigators

This is where you will find people and organizations involved with this study.

Sponsor

University Hospital, Grenoble

Investigators

  • Principal Investigator: Stéphane Doutreleau, PhD, University Hospital, Grenoble

Publications and helpful links

The person responsible for entering information about the study voluntarily provides these publications. These may be about anything related to the study.

General Publications

Study record dates

These dates track the progress of study record and summary results submissions to ClinicalTrials.gov. Study records and reported results are reviewed by the National Library of Medicine (NLM) to make sure they meet specific quality control standards before being posted on the public website.

Study Major Dates

Study Start (Actual)

May 9, 2016

Primary Completion (Actual)

September 1, 2021

Study Completion (Actual)

September 1, 2021

Study Registration Dates

First Submitted

May 18, 2016

First Submitted That Met QC Criteria

July 26, 2016

First Posted (Estimated)

August 1, 2016

Study Record Updates

Last Update Posted (Estimated)

December 29, 2025

Last Update Submitted That Met QC Criteria

December 19, 2025

Last Verified

December 1, 2025

More Information

Terms related to this study

Additional Relevant MeSH Terms

Other Study ID Numbers

  • 38RC14.430

Plan for Individual participant data (IPD)

Plan to Share Individual Participant Data (IPD)?

NO

This information was retrieved directly from the website clinicaltrials.gov without any changes. If you have any requests to change, remove or update your study details, please contact register@clinicaltrials.gov. As soon as a change is implemented on clinicaltrials.gov, this will be updated automatically on our website as well.

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