Multi-elemental Imaging of Lung Tissues With LIBS (Laser-induced Breakdown Spectroscopy) (MEDICO-LIBS)

Multi-elemental Imaging of Lung Tissues With LIBS (Laser-induced Breakdown Spectroscopy) : a Feasibility Study

Evaluate the feasibility of performing a multi-elemental imaging analysis of lung specimens from patients with ILDs, with an technology named LIBS (Laser Induced-Breakdown Spectroscopy)

Study Overview

• Status: Completed
• Conditions: Idiopathic Pulmonary Fibrosis; Interstitial Lung Disease; Sarcoidosis
• Intervention / Treatment: Diagnostic test: Elemental analysis

Detailed Description

There is a major health problem due to the environmental and occupational related exposures to mineral particles, metals, and dust. The impact on health is considerable in terms of prevalence, morbidity and healthcare costs. Pathologists rarely report the presence of a possible mineral or metal-related etiology in a specimen by lack of available and convenient technology. Therefore, several respiratory diseases are considered idiopathic.

Interstitial lung disease (ILD), also known as diffuse parenchymal lung disease (DPLD) is a group of lung diseases affecting the interstitium (the tissue and space around the air sacs of the lungs). In France, 15.000 new cases of ILD are diagnosed each year. ILD may be classified according to the cause. Idiopathic ILD are ILD of unknown causes. The most frequent idiopathic ILD are sarcoidosis and idiopathic pulmonary fibrosis (IPF).These two diseases are the core topic of the present study since they may relate to important mineral or metal exposure.

The overall goal of this MEDICO-LIBS study is to evaluate the feasibility of using a laser-induced breakdown spectroscopy (LIBS)-based instrument to image(ie, identify, localize and possibly quantify) metals within selected human specimens with ILDs.

• Study Type: Observational
• Enrollment (Actual): 86

Contacts and Locations

Study Locations

• France
  • Grenoble, France, 38043
    • Grenoble Alpes University Hospital
  • Lille, France, 59000
    • CHRU de Lille
  • Lyon, France, 69677
    • Hôpital Louis Pradel
  • Lyon, France, 69310
    • CHU de Lyon HCL - GH Sud

Participation Criteria

Eligibility Criteria

• Ages Eligible for Study: 18 years and older (Adult, Older Adult)
• Accepts Healthy Volunteers: No

• Sampling Method: Non-Probability Sample

Study Population

Adults with Interstitial Lung Disease

Description

Inclusion Criteria:

• Patient with ILD
• Patient informed, and agreed to participate

Exclusion Criteria:

• Patient opposed to participating in the study.
• Patient without available specimen.
• Patient under legal protection

Study Plan

How is the study designed?

Number of groups / cohorts

1

Cohorts and Interventions

Group / Cohort	Intervention / Treatment
Interstitial Lung Disease; Ex : sarcoidosis, IPF	Diagnostic test: Elemental analysis; LIBS multi elemental analysis of the specimen

What is the study measuring?

Primary Outcome Measures

Outcome Measure	Measure Description	Time Frame
Generation of multi-elemental images in tissues with LIBS	Evaluation of the feasibility of collecting and describing LIBS multi-elemental images of lung specimens originating from patients with ILDs	Within a month after reception of the biopsy by the analytical laboratory

Secondary Outcome Measures

Outcome Measure	Measure Description	Time Frame
Description of elemental distribution in the specimens	Comprehensive chemical characterization of both endogenous and exogenous elements for each specimen	Within a month after reception of the biopsy by the analytical laboratory
Interpretation of the elemental maps	Understanding of the elemental distribution according to the clinical data	Within a month after reception of the biopsy by the analytical laboratory
Comparison of the elemental distributions with results of other mineralogical investigations	Only for patients who previously benefited from other mineralogical investigations (eg, SEM-EDX), comparison of mineralogical analysis obtained with the elemental distributions obtained with LIBS	Within a month after reception of the biopsy by the analytical laboratory

Collaborators and Investigators

• Sponsor: University Hospital, Grenoble
• Investigators: Principal Investigator: Benoit Busser, PharmD, PhD, Grenoble Alpes University Hospital

Publications and helpful links

General Publications

• Busser B, Moncayo S, Trichard F, Bonneterre V, Pinel N, Pelascini F, Dugourd P, Coll JL, D'Incan M, Charles J, Motto-Ros V, Sancey L. Characterization of foreign materials in paraffin-embedded pathological specimens using in situ multi-elemental imaging with laser spectroscopy. Mod Pathol. 2018 Mar;31(3):378-384. doi: 10.1038/modpathol.2017.152. Epub 2017 Nov 17.
• Busser et al., Coord Chem Rev, 2018, 358, p70

Study record dates

Study Major Dates

• Study Start (Actual): January 22, 2020
• Primary Completion (Actual): April 9, 2024
• Study Completion (Actual): April 9, 2024

Study Registration Dates

• First Submitted: March 28, 2019
• First Submitted That Met QC Criteria: April 1, 2019
• First Posted (Actual): April 3, 2019

Study Record Updates

• Last Update Posted (Actual): August 30, 2024
• Last Update Submitted That Met QC Criteria: August 29, 2024
• Last Verified: August 1, 2024

More Information

Terms related to this study

• Keywords: Laser Induced Breakdown Spectroscopy; Multi elemental imaging
• Additional Relevant MeSH Terms: Pathologic Processes; Respiratory Tract Diseases; Immune System Diseases; Lymphoproliferative Disorders; Lymphatic Diseases; Hypersensitivity; Fibrosis; Hypersensitivity, Delayed; Lung Diseases; Pulmonary Fibrosis; Idiopathic Pulmonary Fibrosis; Lung Diseases, Interstitial; Sarcoidosis
• Other Study ID Numbers: 38RC18.134; 2019-A00070-57 (Other Identifier: ID RCB)

Plan for Individual participant data (IPD)

• Plan to Share Individual Participant Data (IPD)?: NO

Drug and device information, study documents

• Studies a U.S. FDA-regulated drug product: No
• Studies a U.S. FDA-regulated device product: No