- ICH GCP
- US Clinical Trials Registry
- Clinical Trial NCT04113122
Senescence and the Early Ageing Phenotype After Chemotherapy for Testicular Cancer: the SEA-CAT Study
Study Overview
Status
Conditions
Intervention / Treatment
Study Type
Enrollment (Anticipated)
Phase
- Not Applicable
Contacts and Locations
Study Contact
- Name: J. A. Gietema, Prof.
- Phone Number: +31 50 361 2821
- Email: j.a.gietema@umcg.nl
Study Locations
-
-
-
Groningen, Netherlands, 9713 GZ
- Recruiting
- University Medical Center Groningen
-
Contact:
- J. A. Gietema, Prof.
- Phone Number: +31 50 361 2821
- Email: j.a.gietema@umcg.nl
-
Principal Investigator:
- J. A. Gietema, Prof.
-
-
Participation Criteria
Eligibility Criteria
Ages Eligible for Study
Accepts Healthy Volunteers
Genders Eligible for Study
Description
Inclusion Criteria:
In order to be eligible to participate in the cross-sectional part of this study, a subject must meet all of the following criteria:
- Diagnosed with metastatic testicular cancer in 1999-2012 (stage II or higher)
- Received first-line cisplatin-based chemotherapy
- Was younger than 50 years of age at start of chemotherapy
In order to be eligible to participate in the longitudinal part of this study, a subject must meet all of the following criteria:
Chemotherapy-group:
- Diagnosis of metastatic testicular cancer (stage II or higher)
- Is about to start with first-line cisplatin-based chemotherapy
- Younger than 50 years of age at diagnosis of metastatic testicular cancer
Stage I control-group:
- Diagnosis of testicular cancer stage I disease
- Younger than 50 years of age at diagnosis of testicular cancer
Exclusion Criteria:
A potential subject who meets any of the following criteria will be excluded from participation in this study:
- Not able to provide informed consent (in example in case of mental or psychiatric disability)
Study Plan
How is the study designed?
Design Details
- Primary Purpose: Prevention
- Allocation: Non-Randomized
- Interventional Model: Factorial Assignment
- Masking: None (Open Label)
Arms and Interventions
Participant Group / Arm |
Intervention / Treatment |
---|---|
Experimental: Cross-sectional study:
Testicular cancer survivors who were treated between 2000 and 2005 or between 2006 and 2012 with cisplatin-combination chemotherapy and who were extensively phenotypically mapped within two longitudinal trials (15,16) will be invited to participate in a single cross-sectional follow-up study visit 5-20 years after chemotherapy.
|
A 4 mm skin biopsy will be performed at the upper leg of the patient.
Before the skin biopsy local anesthesia is applied subcutaneously.
In these skin biopsies senescent cells will be detected by p16, p21 and yH2Ax staining.
Furthermore, we will measure platinum levels in the skin biopsies.
An abdominal subcutaneous fat biopsy will be performed 7-10 cm on the right side of the umbilicus. Before the fat biopsy local anesthesia is applied subcutaneously. An amount of 30 mg fat tissue will be collected using needle aspiration. In these fat biopsies senescent cells will be detected by p16, p21 and yH2Ax staining. Furthermore, we will measure platinum levels (ICP-MS), adipocytokines (leptin, adiponectin, interleukin-6, PAI-1, TNF-α), p53 activation indirectly by measuring p21 or mdm2 expression using immunohistochemistry, microRNA regulation of insulin signaling in adipose tissue: miR-103, miR-107, miR-29. |
Experimental: Longitudinal study - chemotherapy group
Patients with metastasized testicular cancer who are about to start with cisplatin-combination chemotherapy will be invited in the longitudinal part of this study. Study participation involves four study visits: Visit 1: before start of chemotherapy Visit 2:before third cycle of chemotherapy Visit 3: one month after completion of chemotherapy Visit 4: one year after start of chemotherapy |
A 4 mm skin biopsy will be performed at the upper leg of the patient.
Before the skin biopsy local anesthesia is applied subcutaneously.
In these skin biopsies senescent cells will be detected by p16, p21 and yH2Ax staining.
Furthermore, we will measure platinum levels in the skin biopsies.
An abdominal subcutaneous fat biopsy will be performed 7-10 cm on the right side of the umbilicus. Before the fat biopsy local anesthesia is applied subcutaneously. An amount of 30 mg fat tissue will be collected using needle aspiration. In these fat biopsies senescent cells will be detected by p16, p21 and yH2Ax staining. Furthermore, we will measure platinum levels (ICP-MS), adipocytokines (leptin, adiponectin, interleukin-6, PAI-1, TNF-α), p53 activation indirectly by measuring p21 or mdm2 expression using immunohistochemistry, microRNA regulation of insulin signaling in adipose tissue: miR-103, miR-107, miR-29. |
Other: Longitudinal study - stage I control group
Patients with stage I testicular cancer will serve as control group with three study visits: Visit 1: at time of orchidectomy Visit 2: one month Visit 3: one year after orchidectomy |
A 4 mm skin biopsy will be performed at the upper leg of the patient.
Before the skin biopsy local anesthesia is applied subcutaneously.
In these skin biopsies senescent cells will be detected by p16, p21 and yH2Ax staining.
Furthermore, we will measure platinum levels in the skin biopsies.
An abdominal subcutaneous fat biopsy will be performed 7-10 cm on the right side of the umbilicus. Before the fat biopsy local anesthesia is applied subcutaneously. An amount of 30 mg fat tissue will be collected using needle aspiration. In these fat biopsies senescent cells will be detected by p16, p21 and yH2Ax staining. Furthermore, we will measure platinum levels (ICP-MS), adipocytokines (leptin, adiponectin, interleukin-6, PAI-1, TNF-α), p53 activation indirectly by measuring p21 or mdm2 expression using immunohistochemistry, microRNA regulation of insulin signaling in adipose tissue: miR-103, miR-107, miR-29. |
What is the study measuring?
Primary Outcome Measures
Outcome Measure |
Measure Description |
Time Frame |
---|---|---|
Cellular senescence
Time Frame: 1 year
|
The change in the amount of senescent cells in skin and fat tissue (defined as % of cells in which nucleus is stained positive for P16, P21 and yH2Ax)
|
1 year
|
Secondary Outcome Measures
Outcome Measure |
Measure Description |
Time Frame |
---|---|---|
Senescence-associated secretory phenotype (SASP)
Time Frame: 1 year
|
Change in levels of the cytokines: IL-6, IL-8, VEGF
|
1 year
|
Pulse-wave velocity
Time Frame: 1 year
|
Presence or development of the early ageing phenotype will be assessed measuring vascular damage: change in vascular stiffness (pulse-wave velocity, PWV).
|
1 year
|
Platinum levels
Time Frame: 1 year
|
Changes in circulating platinum levels and the amount of platinum depositions in skin and fat tissure will be assessed.
|
1 year
|
Adipocytokines 1
Time Frame: 1 year
|
Changes in levels of leptin and PAI-1 (ug/L)
|
1 year
|
Adipocytokines 2
Time Frame: 1 year
|
Changes in levels of adiponectin (ug/mL)
|
1 year
|
Collaborators and Investigators
Collaborators
Investigators
- Principal Investigator: J. A. Gietema, Prof., University Medical Center Groningen
Study record dates
Study Major Dates
Study Start (Actual)
Primary Completion (Anticipated)
Study Completion (Anticipated)
Study Registration Dates
First Submitted
First Submitted That Met QC Criteria
First Posted (Actual)
Study Record Updates
Last Update Posted (Actual)
Last Update Submitted That Met QC Criteria
Last Verified
More Information
Terms related to this study
Additional Relevant MeSH Terms
Other Study ID Numbers
- 201700615
Drug and device information, study documents
Studies a U.S. FDA-regulated drug product
Studies a U.S. FDA-regulated device product
This information was retrieved directly from the website clinicaltrials.gov without any changes. If you have any requests to change, remove or update your study details, please contact register@clinicaltrials.gov. As soon as a change is implemented on clinicaltrials.gov, this will be updated automatically on our website as well.
Clinical Trials on Testicular Cancer
-
Haukeland University HospitalKarolinska University Hospital; Oslo University Hospital; University Hospital... and other collaboratorsRecruitingSeminoma | Testicular Germ Cell Cancer | Non-Seminoma Testicular Cancer | Stage I Testicular Cancer | Stage II Testicular Cancer | Stage III Testicular Cancer | Stage IV Testicular Cancer | Relapse Testicular CancerNorway, Sweden
-
University Medical Center GroningenDutch Cancer SocietyCompletedNon-seminomatous Testicular CancerNetherlands
-
SWOG Cancer Research NetworkNational Cancer Institute (NCI)RecruitingSeminoma | Germ Cell Tumor | Metachronous Malignant Neoplasm | Stage I Testicular Cancer AJCC v8 | Stage IA Testicular Cancer AJCC v8 | Stage IB Testicular Cancer AJCC v8 | Stage IS Testicular Cancer AJCC v8Canada, United States, Guam
-
National Cancer Institute, SlovakiaCompletedTesticular CancerSlovakia
-
University of California, IrvineMemorial Sloan Kettering Cancer CenterUnknown
-
University of Texas Southwestern Medical CenterActive, not recruitingTesticular Germ Cell Tumor | Testicular Neoplasms | Testicular Diseases | Testis Cancer | Testicular Cancer | Germ Cell Tumor | Testicular Yolk Sac Tumor | Testicular Choriocarcinoma | Germ Cell Tumor of Testis | Germ Cell Tumor, Testicular, Childhood | Germ Cell Cancer Metastatic | Germ Cell Neoplasm of Retroperitoneum and other conditionsUnited States
-
University Medical Center GroningenCompleted
-
Centre Leon BerardNational Cancer Institute, FranceNot yet recruitingMetastatic Testicular CancerFrance
-
Slovak Academy of SciencesComenius UniversityActive, not recruiting
-
Radboud University Medical CenterCompletedTesticular CancerNetherlands
Clinical Trials on Skin biopsy
-
Hordinsky, Maria K., MDTerminated
-
Sheffield Teaching Hospitals NHS Foundation TrustUniversity of Sheffield; Sheffield Children's NHS Foundation TrustRecruiting
-
Central Hospital, Nancy, FranceNot yet recruitingAlzheimer DiseaseFrance
-
Sohag UniversityNot yet recruiting
-
Fondazione Policlinico Universitario Agostino Gemelli...Recruiting
-
Central Hospital, Nancy, FranceNot yet recruitingHuntington Disease
-
Johns Hopkins UniversityNot yet recruitingSmall Fiber Neuropathy | Gastrointestinal DysmotilityUnited States
-
Hospices Civils de LyonRecruiting
-
Spectrum Health HospitalsVan Andel Research InstituteRecruitingNeurofibromatosis Type 1 | Plexiform Neurofibromas | Cutaneous NeurofibromasUnited States
-
ApteeusEnrolling by invitationMonogenic DisordersFrance