Study of Adjuvant Immunotherapy With EVX-02 and Anti-PD-1

A Phase 1/2, Study of Adjuvant Immunotherapy With EVX-02 and Anti-PD-1 After Complete Resection of Stage IIIB/IIIC/IIID or Stage IV Melanoma in Patients at High Risk for Recurrence

This is a Phase 1/2, open label, multi-centre study to assess the safety, tolerability, PD, and efficacy of adjuvant immunotherapy EVX-02 vaccine and anti-PD-1 (Nivolumab) in patients who have had a complete resection of a Stage IIIB/IIIC/IIID or Stage IV melanoma who are at high risk of recurrence.

Study Overview

• Status: Terminated
• Conditions: Melanoma Stage IV; Melanoma Stage III
• Intervention / Treatment: Drug: EVX-02A; Drug: EVX-02B; Drug: EVX-02A OR EVX-02B

Detailed Description

This study will be conducted in two parts: a safety, efficacy and PD response part using two different drug delivery methods and an expansion cohort.

All patients will be administered with anti-PD-1 once every 4 weeks for up to 1 year commencing on Day 1 and with EVX-02 vaccine as soon as it is produced.

Part 1 will consist of two cohorts:

Cohort A: Will receive EVX- 02A delivered by delivery methodology 1. Cohort B: Will receive EVX- 02B delivered by delivery device 2. Part 2 (Cohort C) will be an expansion cohort. In this part, only one delivery methods, either EVX-02A or EVX-02B with will be used.

• Study Type: Interventional
• Enrollment (Actual): 15
• Phase: Phase 2; Phase 1

Contacts and Locations

Study Locations

• Australia
  • New South Wales
    • Goulburn, New South Wales, Australia, 2170
      • Liverpool Hospital
  • South Australia
    • Adelaide, South Australia, Australia, 5042
      • Flinders Medical Centre
  • Victoria
    • Clayton, Victoria, Australia, 3168
      • Monash Medical Centre
    • Drummond, Victoria, Australia, 3350
      • Ballarat Health Services
    • Melbourne, Victoria, Australia, 3000
      • Peter MacCallum Cancer Centre
  • Western Australia
    • Nedlands, Western Australia, Australia, 6009
      • Linear Cancer Trials

Participation Criteria

Eligibility Criteria

• Ages Eligible for Study: 18 years and older (Adult, Older Adult)
• Accepts Healthy Volunteers: No

Description

Inclusion Criteria:

1. Signed and dated written ICF prior to performing any protocol-related procedures, which are not part of normal standard care.
2. Patients must be willing and able to comply with scheduled visits, treatment schedule, laboratory tests, tumour sample including core needle biopsy as required, and other requirements of the study.
3. Male or female ≥ 18 years of age at the time of informed consent.

4. Stage IIIB/IIIC/IIID or Stage IV AJCC (8th edition) (AJCC, 2018) before complete resection.

   Please note: If Stage III melanoma (whether Stage IIIB or IIIC or IIID) the patients must have clinically detectable lymph nodes that are confirmed as malignant on the pathology report and/or ulcerated primary lesions, and/or in transit metastasis. The pathology report must be reviewed, signed and dated by the Investigator; this process must be done prior to enrolling patients into the study. Clinically detectable lymph nodes are defined as:

   1. a palpable node (confirmed as malignant by pathology) after the CLND
   2. a non-palpable but enlarged lymph node by CT scan (at least 15 mm in short axis) and confirmed as malignant by pathology after the CLND
   3. a PET scan positive lymph node of any size confirmed by pathology after CLND
   4. evidence of pathologically macro metastatic disease in one or more lymph nodes defined by one or more foci of melanoma at least 1 cm in diameter If Stage IV melanoma, the pathology report confirming negative margins must be reviewed, dated, and signed by the Investigator prior to randomisation.
5. Cutaneous Melanoma, with metastases to regional lymph nodes or distant metastases, or in transit metastases, that can be surgically resected with negative margin on resected specimens.
6. Specimen from the resected tumour tissue must be provided for evaluation by NGS.
7. ECOG performance status score of 0 or 1.

8. Screening laboratory values must meet the following criteria and must be reconfirmed for eligibility within 72 hours prior to first dose of anti-PD-1 on Day 1:

   i. WBCs ≥ 2000/μL (2.0 x 109/L) ii. Neutrophils ≥ 1500/μL (1.5 x109/L) iii. Platelets ≥ 100 x 10³/μL (100 x 109/L) iv. Haemoglobin ≥ 9.0 g/dL (90 g/L) v. Creatinine Serum creatinine ≤ 1.5 x ULN or creatinine clearance > 40 mL/minute (using Cockcroft/Gault formula) vi. AST ≤ 3 x ULN vii. ALT ≤ 3 x ULN viii. Total Bilirubin ≥ 1.5 x ULN (except patients with Gilbert Syndrome who must have total bilirubin < 3.0 mg/dL)

9. Life expectancy > 6 months at Screening.
10. WOCBP and men must agree to use adequate contraception (hormonal or barrier method of birth control, abstinence) prior to study entry and for the duration of study participation. Should a woman become pregnant or suspect she is pregnant while participating in this study, she should inform her treating physician immediately.
11. WOCBP must have a negative serum or urine pregnancy test (minimum sensitivity 25 IU/L or equivalent units of hCG]) within 24 hours prior to first dose of anti-PD-1 on Day 1.
12. WOCBP must agree to follow instructions for method(s) of contraception for the duration of treatment with study drug (s) plus 5 half-lives of study drug plus 30 days (duration of ovulatory cycle). The half-life of nivolumab is up to 25 days. WOCBP should therefore use an adequate method to avoid pregnancy for a total of 23 weeks post-treatment completion.
13. Men who are sexually active with a WOCBP must agree to follow instructions for method(s) of contraception for the duration of treatment with study drug(s) plus 5 half-lives of the study drug(s) plus 90 days (duration of sperm turnover). The half-life of nivolumab is up to 25 days, men should therefore use an adequate method of contraception for a total of 31 weeks post-treatment completion.
14. Azoospermic males and WOCBP who are continuously not heterosexually active are exempt from contraceptive requirements. However, WOCBP must still undergo pregnancy testing.
15. Disease-free at study enrolment (after surgery) based upon pathology report review by the Investigator.

Exclusion Criteria:

1. Known or active other malignancies that have needed treatment (excluding palliative radiation) within 2 years prior to Cycle 1 Day 1 are excluded except for the following:

   1. Curatively resected non-melanomatous skin cancer
   2. Curatively treated cervical carcinoma in situ
   3. Non-metastatic breast and prostate cancer patients with stable disease > 12 months
   4. Other stable malignancies may be permitted based on case-by-case discussions with Sponsor and Medical Monitor approval.
2. Patients with clinically occult lymph nodes (i.e. detected by SLN biopsy) stage N1a and N2a.
3. Participated in any other investigational study, unless treatment in that study has been discontinued at least 30 days or period of five half-lives prior to Screening.
4. Known or suspected allergy or hypersensitivity to any of the therapeutic agents to be administered during the study.
5. Uncontrolled concurrent illness including, but not limited to, active infection, symptomatic congestive heart failure or cardiac arrhythmia.
6. Has a history of clinically significant GI disease, renal, hepatic, neurologic, haematologic, endocrine, oncologic, pulmonary, immunologic, or cardiovascular disease or any other condition which, in the opinion of the Investigator, would jeopardise the safety of the patient or impact the validity of the study results.
7. Uncontrolled mental disease or psychotic manifestation that, in the opinion of the Investigator, would prohibit compliance with the protocol, the understanding of the ICF, or the ability to withdraw from the study.
8. Known history of or positive test for HIV or known immunodeficiency syndrome (AIDS).
9. For patients with evidence of chronic HBV infection, the HBV viral load must be undetectable on suppressive therapy, if indicated.
10. Patients with a history of HCV infection must have been treated and cured. For patients with HCV infection who are currently on treatment, they are eligible if they have an undetectable HCV viral load.
11. Pregnant or nursing women.
12. Ocular or uveal melanoma.
13. History of carcinomatosis meningitis.
14. History of Grade ≥ 3 allergy to human monoclonal antibodies.
15. Active, known, or suspected autoimmune disease or immunosuppressive conditions with the exception of vitiligo, type 1 diabetes, residual autoimmune-related hypothyroidism requiring hormone replacement, or psoriasis not requiring systemic treatment.
16. Prior treatment with immune-modulatory agents including, but not limited to: IL-2, CTLA-4 blockade, PD-1/PD-L1 blockade, CD40 stimulation, CD137 stimulation - with the exception of IFN-α given as adjuvant treatment for high risk, surgically resected melanoma (Note. IFN-α therapy must have been discontinued more than 4 weeks prior to enrolment in the study).
17. Prior investigational melanoma-directed cancer vaccine therapy.
18. Prior chemotherapy, including targeted therapy such as BRAF or MEK inhibition.
19. Use of a non-oncology vaccine therapy for prevention of infectious diseases up to 4 weeks prior to enrolment in the study.
20. Patients with a condition requiring systemic treatment with either corticosteroids (>10 mg daily prednisone equivalent) or other immunosuppressive medications within 14 days prior to first dose of anti-PD-1 on Day 1. Inhaled or topical steroids are permitted in the absence of active autoimmune disease.

Study Plan

How is the study designed?

Design Details

• Primary Purpose: Treatment
• Allocation: Non-Randomized
• Interventional Model: Parallel Assignment
• Masking: None (Open Label)

Number of Arms

3

Arms and Interventions

Participant Group / Arm	Intervention / Treatment
Experimental: Cohort A: Nivolumab and EVX-02A; EVX-02A administered IM.	Drug: EVX-02A; Up to 8 patients will receive EVX-02A administered IM.; Other Names: A
Experimental: Cohort B: Nivolumab and EVX-02B; EVX-02B administered IM.	Drug: EVX-02B; Up to 8 patients will receive EVX-02B administered IM.; Other Names: B
Experimental: Cohort C: Nivolumab and EVX-02A OR Nivolumab and EVX-02B; The selected delivery methodology either EVX02A or EVX-02B.	Drug: EVX-02A OR EVX-02B; EVX-02A or EVX-02B will be used. Patients: 24 to 30; Other Names: C

What is the study measuring?

Primary Outcome Measures

Outcome Measure	Measure Description	Time Frame
Safety and tolerability (Incidence of Treatment-Emergent Adverse Events)	Measure through CTCAE version 5.0	Measurements at Baseline through study completion, an average of 1 year
Safety and tolerability (Incidence of Treatment-Emergent Adverse Events) measure through Vital Sign- blood pressure	Measured by result of the Vital Sign- blood pressure	Measurements at Baseline through study completion, an average of 1 year
Safety and tolerability (Incidence of Treatment-Emergent Adverse Events) measure through Vital Sign-heart rate	Measured by result of the Vital Sign- heart rate	Measurements at Baseline through study completion, an average of 1 year
Safety and tolerability (Incidence of Treatment-Emergent Adverse Events) measure through Physical exam	Measured by result of the physical exam which includes general appearance, skin, eyes/ears/nose/throat, head and neck.	Measurements at Baseline through study completion, an average of 1 year
Pharmacodynamic response (PD) of EVX-02 assessed by IFN-y ELISPOT	The pharmacodynamic (PD) response of EVX-02 will be assessed by detecting vaccine induced circulating neoepitope-specific T cells. Blood samples will be collected prior, during and after immunization and the immunotherapy induced T cell responses will be assayed by IFN-y ELISPOT.	Measurements at Baseline through study completion, an average of 1 year
Pharmacodynamic response (PD) of EVX-02 assessed by MHC I multimer analysis	The pharmacodynamic (PD) response of EVX-02 will be assessed by detecting vaccine induced circulating neoepitope-specific T cells. Blood samples will be collected prior, during and after immunization. The samples will be analyzed by MHC I multimer analyses detecting neoepitope-recognizing CD8+ T cells reported as frequencies of positive cell out of CD8+ T cells.	Measurements at Baseline through study completion, an average of 1 year
Pharmacodynamic response (PD) of EVX-02 by intracellular cytokine staining and flow cytometry	The pharmacodynamic (PD) response of EVX-02 will be assessed by detecting vaccine induced circulating neoepitpe-specific T cells. Blood samples will be collected prior, during and after immunization. The samples will be analysed by flow cytometry to detect vaccine induced intracellular cytokine response, reported as frequencies of neoepitope-reactive CD4+ cells and CD8+ T cells.	Measurements at Baseline through study completion, an average of 1 year

Secondary Outcome Measures

Outcome Measure	Measure Description	Time Frame
Efficacy measure through Relapse-free survival (RFS)	Measured by result of Relapse-free survival (RFS)	Measurements at Baseline through study completion, an average of 1 year

Other Outcome Measures

Outcome Measure	Measure Description	Time Frame
PD response of EVX-02 assessed by monitoring the quantity of cfDNA in plasma	To monitor the plasma level cfDNA, blood sample will be obtained prior, during and after treatment with EVX-02 and the fluctuations of the level cfDNA will be analysed.	Measured at Screening, Day -84 to Day -1, Day 1, Day 84, Day 168, Day 252, Day 336 and early termination

Collaborators and Investigators

• Sponsor: Evaxion Biotech A/S
• Collaborators: Novotech (Australia) Pty Limited
• Investigators: Principal Investigator: Dr Bavanthi Balakrishnar, Liverpool Hospital

Study record dates

Study Major Dates

• Study Start (Actual): July 13, 2020
• Primary Completion (Actual): February 28, 2023
• Study Completion (Actual): March 28, 2023

Study Registration Dates

• First Submitted: June 14, 2020
• First Submitted That Met QC Criteria: June 30, 2020
• First Posted (Actual): July 2, 2020

Study Record Updates

• Last Update Posted (Estimated): February 21, 2024
• Last Update Submitted That Met QC Criteria: February 19, 2024
• Last Verified: February 1, 2024

More Information

Terms related to this study

• Additional Relevant MeSH Terms: Neoplasms by Histologic Type; Neoplasms; Neuroectodermal Tumors; Neoplasms, Germ Cell and Embryonal; Neoplasms, Nerve Tissue; Neuroendocrine Tumors; Nevi and Melanomas; Melanoma
• Other Study ID Numbers: EVX-02-001

Drug and device information, study documents

• Studies a U.S. FDA-regulated drug product: No
• Studies a U.S. FDA-regulated device product: No