Resistance Training Effects on Gut Microbiome and Cardiometabolic Outcomes

Effects of 6-week Resistance Training Intervention on Gut Microbiome and Cardiometabolic Outcomes in Adults With Overweight and Obesity

The overall objective of this study is to investigate the effects of resistance training on the gut microbiome and cardiometabolic outcomes in adults with overweight and obesity.

Study Overview

• Status: Completed
• Conditions: Exercise Intervention
• Intervention / Treatment: Other: Conventional physical activity guidelines; Behavioral: Resistance training

Detailed Description

The overall objective of this study is to investigate the effects of resistance training on the gut microbiome and cardiometabolic outcomes in adults with overweight and obesity.

• Study Type: Interventional
• Enrollment (Actual): 34
• Phase: Not Applicable

Contacts and Locations

Study Locations

• United States
  • Missouri
    • Columbia, Missouri, United States, 65211
      • University of Missouri

Participation Criteria

Eligibility Criteria

• Ages Eligible for Study: 18 years to 35 years (Adult)
• Accepts Healthy Volunteers: Yes
• Genders Eligible for Study: All

Description

Inclusion Criteria:

• Age 18-35 years
• BMI: 25-45 kg/m2
• Sedentary lifestyle
• Consistent diet patterns
• Weight-stable (<4 kg change over the last 3 mo)
• Willingness to adhere to resistance training protocol and not change diet
• Non-smoker >1 year or more

Exclusion Criteria:

• Disabilities preventing adherence to resistance training protocol
• Recent start of medications that affect metabolism or appetite
• Antibiotics
• Diabetes
• Drug therapy for coronary artery disease, peripheral artery disease, congestive heart failure, or dyslipidemia
• Uncontrolled hypertension and blood pressure ≥ 180/110
• Gastrointestinal disease and/or bariatric surgery
• Smoking
• Illicit drug use
• Pregnant or lactating individuals

Study Plan

How is the study designed?

Design Details

• Primary Purpose: Prevention
• Allocation: Randomized
• Interventional Model: Parallel Assignment
• Masking: Single

Number of Arms

2

Arms and Interventions

Participant Group / Arm	Intervention / Treatment
Other: Conventional physical activity guidelines; Participants are given recommendations on physical activity guidelines	Other: Conventional physical activity guidelines; Participants are given recommendations on physical activity guidelines
Experimental: Resistance training; Participants will undergo 6 weeks of supervised resistance training	Behavioral: Resistance training; Participants will undergo 6 weeks of supervised resistance training

What is the study measuring?

Primary Outcome Measures

Outcome Measure	Measure Description	Time Frame
Change in stool microbiome diversity	16s rRNA sequencing	Change over 6 weeks
Change in stool microbiome composition	16s rRNA sequencing	Change over 6 weeks

Secondary Outcome Measures

Outcome Measure	Measure Description	Time Frame
Change in blood pressure	diastolic and systolic blood pressure	change over 6 weeks
Fat mass	body composition	Change over 6 weeks
Fat free mass	body composition	Change over 6 weeks
Change in fasting glucose	Fasting Glucose	change over 6 weeks
Change in glucose regulation	2 hour glucose clearance	change over 6 weeks
Hip circumference	anthropometrics	change over 6 weeks
Waist circumference	anthropometrics	change over 6 weeks
Physical activity	kcalories burned during free living activity measured by accelerometer	change over 6 weeks
Sleep Quality	Measured using actigraphs	change over 6 weeks
Sleep Duration	Measured using actigraphs	change over 6 weeks
24hr appetite ratings	Subjective rating using visual analog scales	Once at baseline and at the end of 6 week intervention
Change in Body mass	Measured in kg	change over 6 weeks
Short chain fatty acids	Acetate, propionate, and butyrate	Change over 6 weeks

Other Outcome Measures

Outcome Measure	Measure Description	Time Frame
Food environment perceptions	subjective and objective questionnaires	baseline and at the end of 6 week intervention

Collaborators and Investigators

• Sponsor: University of Missouri-Columbia
• Investigators: Principal Investigator: Jaapna Dhillon, PhD, University of Missouri-Columbia

Study record dates

Study Major Dates

• Study Start (Actual): May 19, 2021
• Primary Completion (Actual): September 30, 2022
• Study Completion (Actual): September 30, 2022

Study Registration Dates

• First Submitted: May 5, 2021
• First Submitted That Met QC Criteria: May 24, 2021
• First Posted (Actual): May 28, 2021

Study Record Updates

• Last Update Posted (Actual): March 13, 2023
• Last Update Submitted That Met QC Criteria: March 9, 2023
• Last Verified: March 1, 2023

More Information

Terms related to this study

• Keywords: Physical Activity; Resistance Training; Gut Microbiome
• Other Study ID Numbers: 2054484

Plan for Individual participant data (IPD)

• Plan to Share Individual Participant Data (IPD)?: YES
• IPD Plan Description: Individual participant data that underlie the results reported here after deidentification (text, tables, figures, etc.)
• IPD Sharing Time Frame: Immediately following publication. No end date.
• IPD Sharing Access Criteria: Data will be deposited in online data repositories.
• IPD Sharing Supporting Information Type: STUDY_PROTOCOL; ICF

Drug and device information, study documents

• Studies a U.S. FDA-regulated drug product: No
• Studies a U.S. FDA-regulated device product: No