Releasing of Inflammatory Mediators During Retreatment of Root Canals

The Effect of Different File Systems Used in Retreatment on the Release of Inflammatory Mediators

Nonsurgical root canal retreatment may become essential when the initial endodontic treatment fails because of the persistent intracanal or extracanal infections.The aim of this thesis study is to evaluate the effect of ProTaper Universal Retreatment (PTUR), Reciproc blue and XP-endo Finisher R file systems, which are used in the removal of root canal filling materials during retreatment with different motion kinetics on the release of Substance P, Calcitonin Gene-Related Peptide (CGRP), IL-6 and IL-10 inflammatory mediators in the periapical region

Study Overview

• Status: Completed
• Conditions: Inflammatory Response; Root Canal Infection
• Intervention / Treatment: Other: Protaper Universal Retreatment; Other: Reciproc; Other: XP-Endo finisher R

Detailed Description

Seventy-five individuals with asymptomatic and chronic apical periodontitis teeth with retreatment indication were included in the study. Individuals were randomly divided into three groups according to the file system used to remove root canal filling materials (n = 25): ProTaper Universal Retreatment, Reciproc blue and XP-endo Finisher R.After the removal of the previous root canal filling materials, reshaping and disinfection of the root canals, periapical fluid samples were taken from the root tip of the teeth in all three groups. The amount of Substance P, CGRP, IL-6 and IL-10 mediators that cause pain, swelling and flare-up in periapical fluid samples were measured by ELISA test.

• Study Type: Interventional
• Enrollment (Actual): 75
• Phase: Not Applicable

Contacts and Locations

Study Locations

• Turkey
  • Zonguldak, Turkey
    • Faculty of Dentistry of Bulent Ecevit University

Participation Criteria

Eligibility Criteria

• Ages Eligible for Study: 18 years to 68 years (ADULT, OLDER_ADULT)
• Accepts Healthy Volunteers: Yes
• Genders Eligible for Study: All

Description

Inclusion Criteria:

• Patients who had single root canal treated teeth with chronic periapical lesion

Exclusion Criteria:

• Patients who are immunocompromised
• Patients under antibiotic or analgesic medication

Study Plan

How is the study designed?

Design Details

• Primary Purpose: TREATMENT
• Allocation: RANDOMIZED
• Interventional Model: PARALLEL
• Masking: SINGLE

Number of Arms

3

Arms and Interventions

Participant Group / Arm	Intervention / Treatment
EXPERIMENTAL: Retreatment system using in rotational motion; Previous root canal filling materials were removed with D1-D2-D3 retreatment files with using an endodontic motor in rotational motion	Other: Protaper Universal Retreatment; D1-D2-D3 Retreatment files were used in 500 rpm and 3 Ncm with X-Smart endomotor
EXPERIMENTAL: Retreatment system using in reciprocal motion; Previous root canal filling materials were removed with Reciproc 25 file with using an endodontic motor in reciprocal motion	Other: Reciproc; R25 retratment files were used with X-Smart endomotor in reciprocal motion
EXPERIMENTAL: Retreatment system using in additional rotational motion; Previous root canal filling materials were removed with XP-endo finisher r file with using an endodontic motor in rotational motion	Other: XP-Endo finisher R; XP-Endo finisher r file were used in 1000 rpm 1 Ncm with X-Smart endomotor

What is the study measuring?

Primary Outcome Measures

Outcome Measure	Measure Description	Time Frame
change of inflammatory mediator level	The samples were collected from periapical exudate at the end of the root canal retreatment procedure. The samples were analyzed with ELISA method and change of inflammatory mediator levels were evaluated in pg/ml.	24 hours

Collaborators and Investigators

• Sponsor: Bulent Ecevit University
• Investigators: Study Director: Sevinç Aktemur Türker, Bulent Ecevit University

Study record dates

Study Major Dates

• Study Start (ACTUAL): October 1, 2020
• Primary Completion (ACTUAL): March 15, 2021
• Study Completion (ACTUAL): June 1, 2021

Study Registration Dates

• First Submitted: August 31, 2021
• First Submitted That Met QC Criteria: September 2, 2021
• First Posted (ACTUAL): September 9, 2021

Study Record Updates

• Last Update Posted (ACTUAL): September 9, 2021
• Last Update Submitted That Met QC Criteria: September 2, 2021
• Last Verified: September 1, 2021

More Information

Terms related to this study

• Other Study ID Numbers: 2020-27194235-01

Plan for Individual participant data (IPD)

• Plan to Share Individual Participant Data (IPD)?: NO

Drug and device information, study documents

• Studies a U.S. FDA-regulated drug product: No
• Studies a U.S. FDA-regulated device product: No