Non Surgical Periodontal Treatment Effect on Il-17 and Il-18 in Aggressive Periodontitis

Effect of Non Surgical Periodontal Treatment on Clinical Parameters and Gingival Crevicular Fluid Il-17 and Il-18 Levels in Aggressive Periodontitis Patients

clinical parameters and IL-17 and Il-18 GCF levels were measured in 25 aggressive periodontitis patients compared to 25 periodontally healthy individuals.

It was observed that that Clinical parameters and Il-17 and Il-18 levels are higher before treatment but decreased after treatment which suggests role of these interleukins in pathogenesis of aggressive periodontitis .

Study Overview

• Status: Completed
• Conditions: Aggressive Periodontitis
• Intervention / Treatment: Procedure: non surgical periodontal therapy

Detailed Description

Aggressive periodontitis is a type of rapidly progressing periodontal diseases .The three classic primary characteristics of this disease are ; occurring in young healthy patients with rapid attachment loss and familial aggregation .Bacteria-host interactions, insufficiencies in host defenses and possibly a genetic predisposition represent the etiologic factors . Beside the young age of onset, important factors as involvement of host factors and non-plaque-related etiologic factors have also been proposed .

Aim of the study:

Objectives were to compare and correlate the clinical parameters and biochemical parameters (Il-17 and Il-18 levels in GCF) of aggressive periodontitis patients (At baseline and after three months of nonsurgical treatment) versus periodontally healthy subjects at baseline.

Subjects and methods:

Populations included in the study:

A total of fifty individuals were enrolled and categorized as; group A that included twenty five aggressive periodontitis patients and group B which included twenty five periodontally healthy controls.

Clinical parameters:

Plaque index (PI) ,gingival index (GI), Probing pocket depth (PPD) and clinical attachment level (CAL) were measured at baseline for both groups and after three months of non surgical periodontal treatment completion for group A.

GCF sampling:

In group A ,the site with deepest probing depth was selected for GCF sampling while for group B , any site was sampled using PerioPaper strips .

Phase I periodontal treatment After baseline GCF sample collection and clinical measurement recordings, group A patients received phase I periodontal treatment.

Analysis of Il-17 and Il-18 levels in GCF:

GCF samples were examined by enzyme-linked immunosorbent assay (ELISA).Procedures were performed according to the directions in the kits.

• Study Type: Observational
• Enrollment (Actual): 50

Contacts and Locations

Study Locations

• Egypt
  • Cairo, Egypt, 11835
    • Faculty of oral and dental medicine,FUE, Cairo,Egypt

Participation Criteria

Eligibility Criteria

• Ages Eligible for Study: 18 years to 30 years (Adult)
• Accepts Healthy Volunteers: Yes
• Genders Eligible for Study: All

• Sampling Method: Non-Probability Sample

Study Population

Both genders Age between 18 and 30 years old

Description

Inclusion Criteria:

-Medically free subjects.

Exclusion Criteria:

• Subjects with periodontal surgeries in the last 6 months.
• Subjects with prior use of antibiotics in the last 6 months.
• Smokers.
• Pregnant females.
• Lactating females.

Study Plan

How is the study designed?

Number of groups / cohorts

2

Cohorts and Interventions

Group / Cohort	Intervention / Treatment
Aggressive periodontitis; 25 young medically free patients with deep periodontal pockets >5mm	Procedure: non surgical periodontal therapy; Scaling and root planing
Periodontally healthy individuals; 25 young medically free individuals with no periodontal inflammation signs

What is the study measuring?

Primary Outcome Measures

Outcome Measure	Measure Description	Time Frame
Periopaper strips Il-17 and Il-18 GCF measurements	measurement of Il-17 and Il-18 in GCF from aggressive periodontitis patients using periopaper strips	Change from Baseline Il-17 and Il-18 at 3 months

Collaborators and Investigators

• Sponsor: Future University in Egypt
• Investigators: Principal Investigator: Sarah M Elkot, Lecturer, Lecturer of Oral Medicine and Periodontology

Study record dates

Study Major Dates

• Study Start (Actual): March 27, 2021
• Primary Completion (Actual): December 15, 2021
• Study Completion (Actual): December 30, 2021

Study Registration Dates

• First Submitted: March 2, 2022
• First Submitted That Met QC Criteria: March 16, 2022
• First Posted (Actual): March 25, 2022

Study Record Updates

• Last Update Posted (Actual): April 7, 2022
• Last Update Submitted That Met QC Criteria: March 30, 2022
• Last Verified: March 1, 2022

More Information

Terms related to this study

• Keywords: gingival crevicular fluid
• Additional Relevant MeSH Terms: Behavioral Symptoms; Stomatognathic Diseases; Periodontal Diseases; Mouth Diseases; Aggression; Periodontitis; Aggressive Periodontitis
• Other Study ID Numbers: FUE11

Drug and device information, study documents

• Studies a U.S. FDA-regulated drug product: No
• Studies a U.S. FDA-regulated device product: No