The Molecular Pathogenesis Study of Cataract

To study the molecular pathogenesis of cataract and explore possible treatments，anterior lens capsules were collected from cataract patients

Study Overview

• Status: Recruiting
• Conditions: Cataract; Pathogenesis

Detailed Description

Anterior lens capsules were collected from the patients with DC and ARC, and transparent crystals of cadaveric eyes. M6A epitranscriptomic microarray was performed to investigate the altered m6A modifications and determine the differentially expressed mRNAs. The potential role of dysregulated m6A modification levels was predicted through Gene Ontology and Kyoto Encyclopedia of Genes and Genomes pathway enrichment analyses. Real-time polymerase chain reaction was performed to identify the dysregulated expression of RNA methyltransferases, demethylases, and readers.

• Study Type: Observational
• Enrollment (Anticipated): 150

Contacts and Locations

• Study Contact: Name: Lei Cai, MD; Phone Number: 13816956828; Email: clyc0318@126.com

Study Locations

• China
  • Shanghai
    • Shanghai, Shanghai, China, 200031
      • Recruiting
      • Eye & ENT Hospital of Fudan University
      • Contact: Jin Yang, PhD; Phone Number: 13671632525; Email: jin_er76@hotmail.com
      • Principal Investigator: Lei Cai, MD
      • Principal Investigator: Dongmei Ma, MD

Participation Criteria

Eligibility Criteria

• Ages Eligible for Study: 18 years to 90 years (Adult, Older Adult)
• Accepts Healthy Volunteers: No
• Genders Eligible for Study: All

• Sampling Method: Non-Probability Sample

Study Population

Anterior lens capsule tissues from cataract patients with diabetes mellitus more than 10 years, patients with age related cataract, and transparent crystals of cadaveric eyes.

Description

Inclusion Criteria:

• diabetic cataract
• underwent cataract surgery

Exclusion Criteria:

• Combined with other eye diseases
• underwent other eye surgery

Study Plan

How is the study designed?

Design Details

• Observational Models: Case-Control
• Time Perspectives: Prospective

Number of groups / cohorts

3

Cohorts and Interventions

Group / Cohort
DC group; Anterior lens capsule tissues from DC patients
ARC group; Anterior lens capsule tissues from ARC patients
NC group; anterior lens capsules collected from three age-matched transparent crystals of cadaveric eyes

What is the study measuring?

Primary Outcome Measures

Outcome Measure	Measure Description	Time Frame
m6A modification of mRNAs	Epitranscriptomic microarray analysis reveals the differential m6A modification of mRNAs in DC samples	1 month
expression level of mRNAs	the m6A microarray analyses provide data for mRNA expression levels	1 month
levels of methylated mRNA	in vitro validation of the diverse expression levels of methylated mRNA	1 month
levels of RNA methyltransferase	in vitro validation of the diverse expression levels of RNA methyltransferase	1 month

Collaborators and Investigators

• Sponsor: Eye & ENT Hospital of Fudan University
• Collaborators: National Natural Science Foundation of China
• Investigators: Principal Investigator: Jin Yang, PhD, Eye & ENT Hospital

Study record dates

Study Major Dates

• Study Start (Actual): January 1, 2022
• Primary Completion (Anticipated): December 1, 2023
• Study Completion (Anticipated): December 30, 2025

Study Registration Dates

• First Submitted: December 6, 2022
• First Submitted That Met QC Criteria: January 3, 2023
• First Posted (Actual): January 12, 2023

Study Record Updates

• Last Update Posted (Actual): January 12, 2023
• Last Update Submitted That Met QC Criteria: January 3, 2023
• Last Verified: January 1, 2023

More Information

Terms related to this study

• Additional Relevant MeSH Terms: Eye Diseases; Lens Diseases; Cataract
• Other Study ID Numbers: CMP

Plan for Individual participant data (IPD)

• Plan to Share Individual Participant Data (IPD)?: No

Drug and device information, study documents

• Studies a U.S. FDA-regulated drug product: No
• Studies a U.S. FDA-regulated device product: No