Resistance Exercise and Hydrolyzed Collagen Supplementation

The Dose-response of Vitamin C-enriched Collagen on Markers of Collagen Synthesis in Healthy Young and Older Men and Women Following Resistance Exercise

This study aimed to investigate the effect of different doses of hydrolyzed collagen (HC) with resistance exercise (RE) on whole body collagen synthesis in healthy young and older males and females.

Study Overview

• Status: Completed
• Conditions: Diet, Food, and Nutrition
• Intervention / Treatment: Dietary supplement: Experimental: Consumption of three different doses of hydrolyzed collagen (HC) with reistance exercise; Dietary supplement: Experimental: Consumption of two different doses of hydrolyzed collagen (HC) with reistance exercise

Detailed Description

This study aimed to investigate the effect of different doses of hydrolyzed collagen (HC) with resistance exercise (RE) on whole body collagen synthesis in healthy young and older males and females.

Healthy young and older males and females ingested 0 grams, 15 grams or 30 grams HC with 50 milligrams vitamin C 1h prior to performing four sets' barbell back-squat RE at 10-repetition maximum load, after which they rested for six hours. Blood samples were collected throughout each trial to analyse procollagen type Ⅰ N-terminal propeptide (PⅠNP, a biomarker of collagen synthesis) and β-isomerized C-terminal telopeptide of type I collagen (β-CTX, a biomarker of collagen breakdown) concentration, and the concentration of 18 amino acids that constitute collagen.

This is the first study to investigate the combined effect of different doses HC with high-intensity RE on whole body collagen synthesis in healthy young and older men and women. If 30 grams HC intake with RE does augment collagen synthesis more than RE alone, this suggests that long-term HC intake with chronic RE would be beneficial for tendon health, because collagen is the most abundant protein in tendon.

• Study Type: Interventional
• Enrollment (Actual): 21
• Phase: Not Applicable

Contacts and Locations

Study Locations

• United Kingdom
  • Liverpool, United Kingdom, L3 3AF
    • Dr Rob Erskine

Participation Criteria

Eligibility Criteria

• Ages Eligible for Study: Adult; Older Adult
• Accepts Healthy Volunteers: Yes

Description

Inclusion Criteria:

• At least 12 months experienced in resistance exercise (regular back squat experience)
• Free from musculoskeletal injury
• Nullipara (a woman who has never given birth) (only for young female participatns)

Exclusion Criteria:

• Having a history of patellar tendon pathology
• Vegan
• Consumed nutritional supplements or medication purported to have beneficial effects on muscle-tendon properties (e.g. antioxidants, protein, etc.)
• Had a sustained a lower limb injury in the previous six months
• Smoker/vaper
• <18 or >40 years old (only for young participants)
• <40 ro >65 years old (only for older participants)
• Having an irregular menstrual cycle (only for young female participants)

Study Plan

How is the study designed?

Design Details

• Primary Purpose: Basic Science
• Allocation: Randomized
• Interventional Model: Crossover Assignment
• Masking: Triple

Number of Arms

4

Arms and Interventions

Participant Group / Arm	Intervention / Treatment
Experimental: 1.Trial for young male participants: Consuming hydrolyzed collagen (HC) with reistance exercise; Young male participants consumed one of three different HC doses (0 grams, 15 grams, or 30 grams) with 4 sets of 10 repetitions of barbell back squat exercise at 10-repetition maximum load in a random order and a seven-day wash-out period interspersed between each trial.	Dietary supplement: Experimental: Consumption of three different doses of hydrolyzed collagen (HC) with reistance exercise; Each trial lasts for seven hours and while participants consumed HC, performed resistance exercise and rested, 10 x 5 mL blood samples were collected from a superficial forearm vein using a cannula. All trials were performed at the same time of day (08:00 - 15:00). Different doses of HC (0 grams, 15 grams and 30 grams) and 50 milligrams vitamin C were dissolved with 250 milliliter water in an opaque bottle. To match calories of 30 grams HC, 34.1 grams and 15.4 grams maltodextrin was used in 0 grams HC and 15 grams HC respectively. Also to mask any potential difference in HC doses, 4 grams non-caloric sweetener was used in all HC doses. The details of nutritoinal supplements used are as follows: Hydrolysed collagen (Myprotein, Cheshire, UK), Vitamin C powder (Holland and Barrett Retail Limited, Warwickshire, UK), Maltodextrin (Myprotein, Cheshire, UK), and Non-caloric sweetener (Truvia®, SilverSpoon, London, UK); Other Names: Resistance exercise and collagen dose-response for male participants
Experimental: 2.Trial for young female participant: Consuming hydrolyzed collagen (HC) with reistance exercise; The intervention procedure is exactly same as Arm 1 except for the number of visits. Young female participant was asked to visit the laboratory on four occasions during two consecutive months. Therefore, there were two trials in each month, where female participants' estrogen level was lower (i.e., onset of menses) or higher (i.e., ovulation). Dates for the trials were determined based on self-report of onset of menses and previous menstrual cycle length.	Dietary supplement: Experimental: Consumption of two different doses of hydrolyzed collagen (HC) with reistance exercise; Each trial lasts for seven hours and while participants consumed HC, performed resistance exercise and rested, 10 x 5 mL blood samples were collected from a superficial forearm vein using a cannula. All trials were performed at the same time of day (08:00 - 15:00). Different doses of HC (0 grams and 30 grams) and 50 milligrams vitamin C were dissolved with 250 milliliter water in an opaque bottle. To match calories of 30 grams HC, 34.1 grams maltodextrin was used in 0 grams HC. Also to mask any potential difference in HC doses, 4 grams non-caloric sweetener was used in all HC doses. The details of nutritoinal supplements used are as follows: Hydrolysed collagen (Myprotein, Cheshire, UK), Vitamin C powder (Holland and Barrett Retail Limited, Warwickshire, UK), Maltodextrin (Myprotein, Cheshire, UK), and Non-caloric sweetener (Truvia®, SilverSpoon, London, UK); Other Names: Resistance exercise and collagen dose-response for female participants
Experimental: 3.Trial for older male participants: Consuming hydrolyzed collagen (HC) with reistance exercise; The age range of older male participants was 40 - 65 years. The intervention procedure is exactly same as Arm 1	Dietary supplement: Experimental: Consumption of three different doses of hydrolyzed collagen (HC) with reistance exercise; Each trial lasts for seven hours and while participants consumed HC, performed resistance exercise and rested, 10 x 5 mL blood samples were collected from a superficial forearm vein using a cannula. All trials were performed at the same time of day (08:00 - 15:00). Different doses of HC (0 grams, 15 grams and 30 grams) and 50 milligrams vitamin C were dissolved with 250 milliliter water in an opaque bottle. To match calories of 30 grams HC, 34.1 grams and 15.4 grams maltodextrin was used in 0 grams HC and 15 grams HC respectively. Also to mask any potential difference in HC doses, 4 grams non-caloric sweetener was used in all HC doses. The details of nutritoinal supplements used are as follows: Hydrolysed collagen (Myprotein, Cheshire, UK), Vitamin C powder (Holland and Barrett Retail Limited, Warwickshire, UK), Maltodextrin (Myprotein, Cheshire, UK), and Non-caloric sweetener (Truvia®, SilverSpoon, London, UK); Other Names: Resistance exercise and collagen dose-response for male participants
Experimental: 4.Trial for older female participants: Consuming hydrolyzed collagen (HC) with reistance exercise; The age range of older female participants was 40 - 65 years. The intervention procedure is exactly same as Arm 2	Dietary supplement: Experimental: Consumption of two different doses of hydrolyzed collagen (HC) with reistance exercise; Each trial lasts for seven hours and while participants consumed HC, performed resistance exercise and rested, 10 x 5 mL blood samples were collected from a superficial forearm vein using a cannula. All trials were performed at the same time of day (08:00 - 15:00). Different doses of HC (0 grams and 30 grams) and 50 milligrams vitamin C were dissolved with 250 milliliter water in an opaque bottle. To match calories of 30 grams HC, 34.1 grams maltodextrin was used in 0 grams HC. Also to mask any potential difference in HC doses, 4 grams non-caloric sweetener was used in all HC doses. The details of nutritoinal supplements used are as follows: Hydrolysed collagen (Myprotein, Cheshire, UK), Vitamin C powder (Holland and Barrett Retail Limited, Warwickshire, UK), Maltodextrin (Myprotein, Cheshire, UK), and Non-caloric sweetener (Truvia®, SilverSpoon, London, UK); Other Names: Resistance exercise and collagen dose-response for female participants

What is the study measuring?

Primary Outcome Measures

Outcome Measure	Measure Description	Time Frame
Change in a marker of collagen synthesis	Serum procollagen type Ⅰ N-terminal propeptide (PⅠNP) concentrations were measured following 0 grams, 15 grams or 30 grams hydrolyzed collagen (HC) intake with resistnace exercise (RE) during all trials.	at rest immediately prior to HC ingestion, 0.5-hour post RE, 1-hour post RE, 2-hour post RE, 4-hour post RE and 6-hour post RE
Change in a marker of collagen breakdown	Plasma β-isomerized C-terminal telopeptide of type I collagen (β-CTX) concentrations were measured following 0 grams, 15 grams or 30 grams hydrolyzed collagen (HC) intake with resistnace exercise (RE) during all trials.	at rest immediately prior to HC ingestion and 6-hour post RE
Changes in amino acids concentrations in blood	Concentration of serum amino acids that constitute collagen was measured following 0 grams, 15 grams or 30 grams hydrolyzed collagen (HC) intake with resistnace exercise (RE) during all trials.	at rest immediately prior to HC ingestion, 0.5-hour post HC ingestion, 1-hour post HC ingestion, 0.5-hour post RE, 1-hour post RE, 2-hour post RE, 4-hour post RE and 6-hour post RE

Secondary Outcome Measures

Outcome Measure	Measure Description	Time Frame
Estrogen concentrations in women in each trial	Concentrations of estrogen (17β-estradiol) was measured in each trial.	at rest immediately prior to HC ingestion

Collaborators and Investigators

• Sponsor: Rob Erskine
• Collaborators: University of East Anglia
• Investigators: Study Director: Robert M. Erskine, PhD, Liverpool John Moores University; Principal Investigator: Joonsung Lee, MSc, Liverpool John Moores University; Principal Investigator: Christopher Nulty, Liverpool John Moores University

Study record dates

Study Major Dates

• Study Start (Actual): January 1, 2019
• Primary Completion (Actual): August 30, 2019
• Study Completion (Actual): April 30, 2023

Study Registration Dates

• First Submitted: May 26, 2023
• First Submitted That Met QC Criteria: June 27, 2023
• First Posted (Actual): July 6, 2023

Study Record Updates

• Last Update Posted (Actual): July 6, 2023
• Last Update Submitted That Met QC Criteria: June 27, 2023
• Last Verified: June 1, 2023

More Information

Terms related to this study

• Keywords: Resistance exercise; Glycine; Collagen synthesis; Hydolyzed collagen; Proline
• Other Study ID Numbers: 18/SPS/059

Plan for Individual participant data (IPD)

• Plan to Share Individual Participant Data (IPD)?: NO
• IPD Plan Description: No plan to share

Drug and device information, study documents

• Studies a U.S. FDA-regulated drug product: No
• Studies a U.S. FDA-regulated device product: No