Effect of Collagen Peptides, in Combination With Resistance Training, on Body Composition and Muscle Strength in Untrained Men

The effect of resistance training with post-exercise Collagen peptides (CP) supplementation on lean body mass, maximal and explosive, isometric muscle strength as well as fat mass, resting metabolism, health parameters and compared to resistance training alone will be evaluated in a group of untrained overweight male (30-60 yrs) population in a randomized controlled trial (RCT).

Study Overview

• Status: Completed
• Conditions: Body Composition; Muscle Strength; Lean Body Mass; Muscle Power
• Intervention / Treatment: Dietary supplement: Effect of collagen peptides, in combination with resistance training, on body composition and muscle strength compared to placebo in untrained men; Dietary supplement: Placebo Control intervention: Effect of resistance training in combination with placebo (silicon dioxide), on body composition and muscle strength in untrained men

Detailed Description

Participants will perform supervised resistance training involving (Leg press, pull down, chest press, back extension and abdominal crunches) 3 times a week for 12 weeks. Participants will consume either placebo or collagen peptides (in a double blinded study design) just following resistance training on training days (And approximately the same time of day on non-training days). Prior to, and following 12 weeks of exercise, participants will be tested in body composition, maximal and explosive, isometric muscle strength, resting metabolism, health parameters. Additionally, blood samples will be taken to ensure health and training eligibility and safety.

Participants are healthy untrained overweight males (30-60 yrs)

• Study Type: Interventional
• Enrollment (Actual): 80
• Phase: Not Applicable

Contacts and Locations

Study Locations

• Denmark
  • Odense, Denmark, 5000
    • The Department of Sports Science and Clinical Biomechanics at University of Southern Denmark

Participation Criteria

Eligibility Criteria

• Ages Eligible for Study: Adult
• Accepts Healthy Volunteers: Yes

Description

Inclusion Criteria:

• Male, between 30 and 60 years of age
• Fat mass >25 % as measured by bioelectrical impedance analysis
• Stable weight (±5 %) and eating behaviour within the last 3 months.
• Signed informed consent

Exclusion Criteria:

• Regular physical activity >60 min / week
• Stabile or progressive diseases/conditions (including medication) contraindicating or disabling participation in intensive exercise training and/or with potential to affect training adaptation beyond normal variation. For example:
• Contraindications against physical stress corresponding guidelines of American College of Sports Medicine (ACSM)
• Subjective symptoms during exercise (e.g., unusual physical exhaustion, dyspnoea, nausea)
• Moderate to severe mobility limitation (i.e., due to rheumatic disease)
• Diagnosis of cancer within the last 5 years
• Diabetes mellitus I and II uncontrolled arterial hypertension (systolic blood pressure >155 for mmHg and/or diastolic blood pressure >94 mm Hg; mild hypertension under resting condition will be allowed but such participants will be recommended to consult their general practitioner).
• Conditions contraindicating the supplementation protocol.
• Contraindications against nutritional or ergogenic supplements
• Allergy/aversion against animal protein
• Liver or kidney diseases with a non-permission to ingest certain concentrations of protein.

Study Plan

How is the study designed?

Design Details

• Primary Purpose: Other
• Allocation: Randomized
• Interventional Model: Parallel Assignment
• Masking: Double

Number of Arms

2

Arms and Interventions

Participant Group / Arm	Intervention / Treatment
Experimental: Collagen peptide group; This group will consume 1 serving of Collagen peptides, which is produced and marketed ® by GELITA AG, Germany under the brand name BODYBALANC each day.	Dietary supplement: Effect of collagen peptides, in combination with resistance training, on body composition and muscle strength compared to placebo in untrained men; The effects of collagen peptide consumption in combination with resistance training will be tested
Placebo Comparator: Placebo group; This group will consume 1 serving of silicon dioxide (Sipernat 350, Evonik, Germany) each day. The product is absorbed in negligible amounts by the intestine and does therefore induce minor metabolic effects.	Dietary supplement: Placebo Control intervention: Effect of resistance training in combination with placebo (silicon dioxide), on body composition and muscle strength in untrained men; The effects of placebo consumption in combination with resistance training will be tested

What is the study measuring?

Primary Outcome Measures

Outcome Measure	Measure Description	Time Frame
Lean body mass (kg)	Lean body mass measured by dual-energy X-ray absorptiometry (DXA)	Pre (1week prior to intervention) and post (1 week following intervention. i.e 14weeks after inclusion) intervention - about 20 minutes for all dxa-measurements

Secondary Outcome Measures

Outcome Measure	Measure Description	Time Frame
Fat mass (kg)	Fat mass measured by dual-energy X-ray absorptiometry (DXA)	Pre (1 week prior to intervention) and post (1 week following intervention. i.e 14 weeks after inclusion) intervention - about 20 minutes for all dxa-measurements
1 Repetition maximum strength (kg)	1 repetition maximum strength in leg press, pull down and chest press	Pre (1 week prior to intervention) and post (1 week following intervention. i.e 14 weeks after inclusion) intervention - about 25 minutes for all 1RM measurements
Maximal isometric knee extensor force (Nm)	Maximal isometric knee extensor force	Pre (1 week prior to intervention) and post (1 week following intervention. i.e 14 weeks after inclusion) intervention - about 25 minutes for all isokinetic dynamometer measurements
Rate of force development (Nm/sec)	Unilateral rate of force development from onset of force until 0-30, 0-50, 0-100, 0-200ms of the knee extensor	Pre (1 week prior to intervention) and post (1 week following intervention. i.e 14 weeks after inclusion) intervention - about 25 minutes for all isokinetic dynamometer measurements
Knee extensor power (W)	Unilateral knee extensor power (W)	Pre (1 week prior to intervention) and post (1 week following intervention. i.e 14 weeks after inclusion) intervention - about 15 minutes
Sit to stand	30 second sit to stand test	Pre (1 week prior to intervention) and post (1 week following intervention. i.e 14 weeks after inclusion) intervention - about 3 minutes
Full body bone mineral density (g/cm^2)	Full body bone mineral density (BMD) (obtained by DEXA)	Pre (1 week prior to intervention) and post (1 week following intervention. i.e 14 weeks after inclusion) intervention - about 20 minutes for all dxa-measurements
Resting energy expenditure (kcal pr. day)	Resting energy expenditure measured with the ventilated hood and indirect calorimetry	Pre (1 week prior to intervention) and post (1 week following intervention. i.e 14 weeks after inclusion) intervention - 15-25 minutes in total
Blood sampling	Blood samples collected from the antecubital vein in a fasted state. Measurements of Hb1Ac, insulin, glucose, total cholesterol, high density lipoprotein, low density lipoprotein, triglycerides, HOMA-IR, Non-HDL cholesterol (Total - HDL), Total cholesterol / HDL ratio	Pre (1week prior to intervention) and post (1 week following intervention. i.e 14weeks after inclusion) intervention - about 3 minutes

Other Outcome Measures

Outcome Measure	Measure Description	Time Frame
Dietary intake	2*3 days of nutritional intake log	Pre (1 week prior to intervention) and during the intervention period (week 10 if the intervention) - N/A
Systolic and diastolic of the ankle and brachii (mm/Hg)	Blood pressure obtained from the ankle and brachii	Pre (1 week prior to intervention) and post (1 week following intervention. i.e 14 weeks after inclusion) intervention - about 3 minutes
Questionnaires about health status (36-Item Short Form Survey (SF-36))	Questionnaires about health status (SF-36). The SF-36 consists of eight scaled scores from 0-100. The lower the score the more disability. The higher the score the less disability	Pre (1 week prior to intervention) and post (1 week following intervention. i.e 14 weeks after inclusion) intervention - about 5 minutes
Questionnaires about physical activity level (The Short questionnaire to Assess Health-enhancing physical activity (SQUASH))	Questionnaires about activity habits in an average week. Answers can be calculated to metabolic equivalents (MET-units). With larger number of MET-units meaning a more physically active lifestyle	Pre (1 week prior to intervention) and post (1 week following intervention. i.e 14 weeks after inclusion) intervention - about 5 minutes
Compliance to the resistance training protocol (As a percentage of 36 planned sessions)	Compliance to the resistance training protocol	Post (1 week following intervention) - N/A
Compliance to the supplement protocol (As a percentage of the given amount)	Compliance to the supplement protocol	Post (1 week following intervention) - N/A
Height (cm)	Height of participants	Pre (1 week prior to intervention) and post (1 week following intervention. i.e 14 weeks after inclusion) intervention - about 10 minutes for all anthropometric measurements
Weight (kg)	Weight of participants	Pre (1 week prior to intervention) and post (1 week following intervention. i.e 14 weeks after inclusion) intervention - about 10 minutes for all anthropometric measurements
Age (years)	Age of participants	Pre (1 week prior to intervention) intervention - N/A
BMI (body mass index - Height(cm) / weight (kg)^2)	body mass index	Pre (1 week prior to intervention) and post (1 week following intervention. i.e 14 weeks after inclusion) intervention - about 10 minutes for all anthropometric measurements
Waist circumference (cm)	The circumference around the participants' waist at the midway between the lowest rib and the iliac crest at the end of expiration	Pre (1 week prior to intervention) and post (1 week following intervention. i.e 14 weeks after inclusion) intervention - about 10 minutes for all anthropometric measurements
Hip circumference(cm)	The circumference around the participants' waist at the level of the trochanter major	Pre (1 week prior to intervention) and post (1 week following intervention. i.e 14 weeks after inclusion) intervention - about 10 minutes for all anthropometric measurements
Waist-Hip Ratio	Calculated as the waist measurement divided by hip measurement (W⁄H)	Pre (1 week prior to intervention) and post (1 week following intervention. i.e 14 weeks after inclusion) intervention - about 10 minutes for all anthropometric measurements
Ankle and brachial index	The ratio between systolic and diastolic blood pressure between measurements of the ankle and brachii	Pre (1 week prior to intervention) and post (1 week following intervention. i.e 14 weeks after inclusion) intervention - about 10 minutes for all anthropometric measurements

Collaborators and Investigators

• Sponsor: University of Southern Denmark
• Collaborators: CRI Collagen Research Institute GmbH

Study record dates

Study Major Dates

• Study Start (Actual): September 15, 2023
• Primary Completion (Actual): November 23, 2024
• Study Completion (Actual): November 23, 2024

Study Registration Dates

• First Submitted: September 13, 2023
• First Submitted That Met QC Criteria: September 27, 2023
• First Posted (Actual): September 29, 2023

Study Record Updates

• Last Update Posted (Actual): May 5, 2026
• Last Update Submitted That Met QC Criteria: April 28, 2026
• Last Verified: April 1, 2026

More Information

Terms related to this study

• Additional Relevant MeSH Terms: Motor Activity; Movement; Musculoskeletal Physiological Phenomena; Musculoskeletal and Neural Physiological Phenomena; Therapeutics; Diagnostic Techniques and Procedures; Diagnosis; Physical Therapy Modalities; Inorganic Chemicals; Patient Care; Exercise Therapy; Rehabilitation; Aftercare; Continuity of Patient Care; Oxides; Oxygen Compounds; Physical Conditioning, Human; Exercise; Minerals; Physical Examination; Silicon Compounds; Resistance Training; Muscle Strength; Silicon Dioxide
• Other Study ID Numbers: 7260992052

Plan for Individual participant data (IPD)

• Plan to Share Individual Participant Data (IPD)?: NO

Drug and device information, study documents

• Studies a U.S. FDA-regulated drug product: No
• Studies a U.S. FDA-regulated device product: No
• product manufactured in and exported from the U.S.: No