Sea Buckthorn Aqueous Infusion Impact (Sbaii)

Sea Buckthorn Aqueous Infusion Impact on Body Composition Metrics Among Overweight Female Adults.

• A total of 60 overweight women (BMI 25-29.9 kg/m²), aged 18-50years, will be enrolled and randomized equally into intervention and control groups. The intervention group will consume a 5 g freshly prepared seabuckthorn aqueous infusion daily for 12 weeks, while controls maintain usual habits without supplementation.
• The seabuckthorn aqueous infusion will be prepared by using 250mL of potable boiled water and then cooled to a lukewarm temperature (around 40-45°C). At this point, 5 g of seabuckthorn powder will be added, stirred thoroughly until fully dispersed, and consumed immediately. The infusion will be freshly prepared once daily and administered in the morning after breakfast.

Measurements will be conducted using the InBody 270, a validated bioelectrical impedance analyzer employed in this study to obtain accurate assessments of body weight, body mass index (BMI), waist-hip ratio, and visceral fat levels. The device offers segmental analysis and visceral fat estimation through non-invasive, standardized procedures, ensuring consistency and reliability in longitudinal tracking.

Study Overview

• Status: Recruiting
• Conditions: Body Compostition
• Intervention / Treatment: Dietary supplement: Sea buckthorn aqueous infusion

Detailed Description

Background: Overweight and obesity have become major public health challengesdisproportionately affecting adult women worldwide, including Pakistan. Functional foodsare gaining attention for their preventive and therapeutic potential, with Sea buckthornemerging as a promising option due to its rich bioactive composition which exhibit antioxidant, anti-inflammatory, and lipid-regulating properties. This research, therefore, seeks to evaluate the effects of sea buckthorn consumption on female body composition, exploring its role as a nutritional intervention for weight management and metabolic health. Hypothesis: Participants consuming sea buckthorn aqueous infusion showsignificant declines in BMI, body weight, visceral fat, and waist-hip ratio. Methodology : A total of 60 overweight women (BMI 25-29.9 kg/m²), aged 18-50years, will be enrolled and will be randomly allocated to intervention and control groups. Theintervention group will consume freshly prepared seabuckthorn aqueous infusion dailyfor12 weeks, while controls maintain usual habits without supplementation. Data analysis : Data will be analyzed using SPSS version 25. Baseline characteristicsbetween groups will be compared using Descriptive statistics (mean ± SD) as appropriate. Between-group comparisons differences will be analyzed using ANOVA. Statistical significance will be set at p < 0.05. Outcome : Supplementation with seabuckthorn aqueous infusion over a 12-weekperiodwill result in notable improvements in body composition measures among overweight female adults.

• Study Type: Interventional
• Enrollment (Estimated): 60
• Phase: Not Applicable

Contacts and Locations

• Study Contact: Name: Eesha Naeem, Post graduate; Phone Number: +923264095787; Email: eeshanaeem22@gamil.com
• Study Contact Backup: Name: Dr. Qaisar Raza, Phd; Phone Number: +923002479044; Email: qaisar.raza@uvas.edu.pk

Study Locations

• Pakistan
  • Punjab Province
    • Lahore, Punjab Province, Pakistan, 5400
      • Recruiting
      • University of veterinary and animal sciences, Lahore
      • Contact: Dr. Qaisar Raza, Phd; Phone Number: +923002479044; Email: qaisar.raza@gmail.com
      • Principal Investigator: Dr. Qaisar Raza, Phd

Participation Criteria

Eligibility Criteria

• Ages Eligible for Study: Adult
• Accepts Healthy Volunteers: Yes

Description

Inclusion criteria females, age 18-50, bmi greater or equal to 25. exclusion criteria current pregnancy or lactation, a history of chronic metabolic or gastrointestinal diseases, use of weight management drugs or supplements, known allergies to sea buckthorn or other documented intolerances, smoking or alcohol consumption, a physical activity level exceeding 1.4, and prior participation in weight loss research programs

Study Plan

How is the study designed?

Design Details

• Primary Purpose: Treatment
• Allocation: Randomized
• Interventional Model: Parallel Assignment
• Masking: None (Open Label)

Number of Arms

2

Arms and Interventions

Participant Group / Arm	Intervention / Treatment
Placebo Comparator: 1; The participants in the control group will consume placebo that will match in appearance to the supplement and is used to maintain blinding. Participants will consume freshly prepared sea buckthorn aqueous infusion daily for 12 weeks.	Dietary supplement: Sea buckthorn aqueous infusion; Sea buckthorn aqueous infusion prepared by using 5g powder in 250 ml water.
Experimental: 2; Drug (Sea buckthorn aqueous infusion) The participants will consume Sea buckthorn aqueous infusion supplementation daily for 12 weeks. it will be prepared by using 5g sb powder in 250 ml of water.	Dietary supplement: Sea buckthorn aqueous infusion; Sea buckthorn aqueous infusion prepared by using 5g powder in 250 ml water.

What is the study measuring?

Primary Outcome Measures

Outcome Measure	Measure Description	Time Frame
Body weight	unit of measurement in kilograms will be measured by weight machine	12 weeks
Body mass index	body mass index will be measured using body mass index calculator	at the end of intervention (12 week)

Secondary Outcome Measures

Outcome Measure	Measure Description	Time Frame
visceral fat	visceral fat will be measured using bioelectric impedance analysis	at the end of intervention period (12 week)
waist-hip ratio	waist hip ratio will be measured using measuring tape	at the end of intervention period ( 12 week)

Collaborators and Investigators

• Sponsor: University of Veterinary and Animal Sciences, Lahore - Pakistan

Study record dates

Study Major Dates

• Study Start (Estimated): January 1, 2026
• Primary Completion (Estimated): March 30, 2026
• Study Completion (Estimated): April 15, 2026

Study Registration Dates

• First Submitted: December 17, 2025
• First Submitted That Met QC Criteria: December 17, 2025
• First Posted (Estimated): January 2, 2026

Study Record Updates

• Last Update Posted (Estimated): January 2, 2026
• Last Update Submitted That Met QC Criteria: December 17, 2025
• Last Verified: December 1, 2025

More Information

Terms related to this study

• Keywords: body composition; sea buckthorn; aqueous infusion
• Other Study ID Numbers: UVAS2

Plan for Individual participant data (IPD)

• Plan to Share Individual Participant Data (IPD)?: NO

Drug and device information, study documents

• Studies a U.S. FDA-regulated drug product: No
• Studies a U.S. FDA-regulated device product: No