Investigating the Pharmacokinetics of Petrelintide Using Different Drug Product Concentrations

Investigating the Pharmacokinetics of Petrelintide Using Different Drug Product Concentrations in Participants With a BMI ≥27.0 kg/m²

This clinical research study is testing the study compound petrelintide that is being developed for the weight management in people with obesity or overweight with co-morbidities or related diseases.

The goal is to learn how the same single dose of petrelintide, given using different concentrations, works in the body when given to participants with a BMI at, or above, 27kg/m2.

The main questions it aims to answer are:

1. Are there differences in how the body absorbs, distributes, metabolises and excretes petrelintide when same single dose petrelintide is given using different concentrations?
2. Are there differences in safety of petrelintide when same single dose petrelintide is given using different concentrations?

Participants will:

Visit the study center 10 times for tests and blood sampling (9 day ambulatory visits and 1 in-house visit of 4 consecutive days with sleeping 3 nights at the study center).

Petrelintide will be given once as a single injection in a skin fold of the abdomen on Day 1. The participants will be monitored until approximately Day 50.

Study Overview

• Status: Completed
• Conditions: Obesity & Overweight
• Intervention / Treatment: Drug: Petrelintide

Detailed Description

This is an open-label, single-center, randomized, parallel-group study designed to investigate the pharmacokinetic (PK) profiles, safety, and tolerability of a single dose administration of petrelintide administered subcutanously (SC) using 4 different drug product concentrations. The study will be conducted in participants who have a body mass index (BMI) ≥27.0 kg/m2, who will be stratified to 2 cohorts by BMI at randomization (≤38 vs >38, with 40% cap in each group).

Approximately 48 participants are needed for the study.

• Study Type: Interventional
• Enrollment (Actual): 48
• Phase: Phase 1

Contacts and Locations

Study Locations

• Germany
  • Berlin, Germany, 10117
    • Charité Research Organisation GmbH

Participation Criteria

Eligibility Criteria

• Ages Eligible for Study: Adult
• Accepts Healthy Volunteers: Yes

Description

Inclusion Criteria:

• Signed and dated informed consent obtained before any study-related activities. Study-related activities are any procedures that would not have been done during normal management of the participant
• BMI: ≥27.0 kg/m2, at screening and Day -1
• Have sufficient venous access to allow cannulation for blood sampling as required by the protocol.
• Able and willing to comply with all study procedures

Exclusion Criteria:

• HbA1c ≥48 mmol/mol (6.5%)
• Systolic BP ≥160 mmHg or diastolic BP ≥110 mmHg at screening
• History of type 1 or type 2 diabetes mellitus
• Treatment with glucose-lowering agent(s) within 90 days prior to screening
• Obesity induced by an endocrinologic disorder (eg, Cushing Syndrome) or diagnosed monogenetic or syndromic forms of obesity
• Uncontrolled thyroid disease as judged clinically significant by the Investigator
• Receipt of any medicinal product under clinical development within 30 days or at least 5 half-lives of the related substances and their metabolites (whichever is longer) before randomization in this study
• Previous exposure to petrelintide (ZP8396) or exposure to other amylin analogs within the last 3 months
• Evidence of significant neuropsychiatric disease
• Any history or presence of a disorder or a disease, which, in the Investigator's opinion, might jeopardize the participant's safety, evaluation of results, or compliance with the protocol.
• Known or suspected hypersensitivity to study product or related products.
• Known cardiovascular disease (excluding hypertension), including arthrosclerosis, transient ischemic attack, stroke, angina pectoris, or a history of myocardial infarction or coronary arterial bypass graft/percutaneous coronary intervention
• Presence or history of clinically significant arrhythmias or clinically significant conduction disorders
• A marked baseline prolongation of QT/QTc interval (eg, repeated demonstration of a QTc interval >450 ms) at screening or Day -1
• A history of additional risk factors for Torsades de pointes (eg, heart failure, hypokalemia, family history of Long QT syndrome)
• The use of concomitant medications that prolong the QT/QTc interval
• Presence or history of acute or chronic pancreatitis.
• Known clinically significant gastric emptying abnormality (for example severe gastroparesis, gastric outlet obstruction, gastric bypass operations or sleeve gastrectomy) or chronic treatment that affects gastrointestinal motility
• History or presence of malignant neoplasms (except basal or squamous cell skin cancer or cervical carcinoma in situ) within 5 years before the screening as judged by the Investigator
• Planned surgery scheduled during the study period, except for minor surgical procedures, as judged by the Investigator
• Anticipated change in lifestyle (eg, eating, exercise, or sleep pattern) during the study
• Smoking more than 5 cigarettes or the equivalent nicotine consumption per day (including use of nicotine preparations)
• Inability or unwillingness to refrain from smoking and use of nicotine substitute products 1 day before and during the inpatient period
• Any use of chronic (>14 days) systemic glucocorticoid therapy (excluding topical, intraocular, intranasal, single intraarticular injection, or inhaled preparations) within 90 days of screening
• Any medication (prescription and non-prescription drugs) except for stable treatment with antihypertensive and lipid-lowering drugs as well as thyroid replacement therapy, unless approved by the medical monitor in consultation with the Sponsor (Note: Stable treatment with antihypertensive and lipid lowering drugs (stable for ≥1 month prior to screening) as well as thyroid replacement therapy (≥2 months prior to screening) will be allowed. Use of routine vitamins, topical treatments (including local acting steroids/antihistamines for the treatment of mild hay fever), menopausal hormone replacement therapy, and contraceptives are allowed. In addition, occasional use of acetylsalicylic acid, paracetamol, and ibuprofen for acute pain treatment is allowed.

Study Plan

How is the study designed?

Design Details

• Primary Purpose: Basic Science
• Allocation: Randomized
• Interventional Model: Parallel Assignment
• Masking: None (Open Label)

Number of Arms

4

Arms and Interventions

Participant Group / Arm	Intervention / Treatment
Active Comparator: Arm A; Each participant will receive a single subcutaneous dose of petrelintide strength A on Day 1.	Drug: Petrelintide; Solution administered with a syringe; Other Names: ZP8396
Active Comparator: Arm B; Each participant will receive a single subcutaneous dose of petrelintide strength B on Day 1.	Drug: Petrelintide; Solution administered with a syringe; Other Names: ZP8396
Active Comparator: Arm C; Each participant will receive a single subcutaneous dose of petrelintide strength C on Day 1.	Drug: Petrelintide; Solution administered with a syringe; Other Names: ZP8396
Active Comparator: Arm D; Each participant will receive a single subcutaneous dose of petrelintide strength D on Day 1.	Drug: Petrelintide; Solution administered with a syringe; Other Names: ZP8396

What is the study measuring?

Primary Outcome Measures

Outcome Measure	Measure Description	Time Frame
To investigate the Pharmacokinetics of a single dose administration of petrelintide administered subcutaneously using 4 different drug product concentrations in participants with overweight or obesity	Area under the petrelintide plasma concentration time curve from time zero to infinity after a single dose of petrelintide (AUC0-inf)	Day 1 - day 50 (+/- 2)
To investigate the Pharmacokinetics of a single dose administration of petrelintide administered subcutaneously using 4 different drug product concentrations in participants with overweight or obesity	Maximum observed plasma concentration (Cmax) from time zero to infinity after a single dose of petrelintide	Day 1 - day 50 (+/- 2)

Secondary Outcome Measures

Outcome Measure	Measure Description	Time Frame
To investigate the Pharmacokinetics of a single dose administration of petrelintide administered subcutaneously using 4 different drug product concentrations in participants with overweight or obesity	Area under the petrelintide plasma concentration time curve from time zero to last measurable concentration after a single dose of petrelintide (AUC0-t)	Day 1 - day 50 (+/- 2)
To investigate the Pharmacokinetics of a single dose administration of petrelintide administered subcutaneously using 4 different drug product concentrations in participants with overweight or obesity	Time to maximum observed petrelintide plasma concentration after a single dose of petrelintide (Tmax)	Day 1 - day 50 (+/- 2)
To investigate the Pharmacokinetics of a single dose administration of petrelintide administered subcutaneously using 4 different drug product concentrations in participants with overweight or obesity	Terminal elimination half-life for petrelintide after a single dose of petrelintide (t1/2)	Day 1 - day 50 (+/- 2)
To investigate the Pharmacokinetics of a single dose administration of petrelintide administered subcutaneously using 4 different drug product concentrations in participants with overweight or obesity	Apparent clearance of petrelintide after a single dose of petrelintide (CL/F)	Day 1 - day 50 (+/- 2)
To investigate the safety and tolerability of a single dose administration of petrelintide administered subcutaneously using 4 different drug product concentrations in participants with overweight or obesity	Incidence of treatment-emergent adverse events (TEAEs) from dosing (Day 1) to end of follow-up	Day 1 - day 50 (+/- 2)

Collaborators and Investigators

• Sponsor: Zealand Pharma
• Collaborators: ICON Clinical Research
• Investigators: Principal Investigator: Martha Haidacher, Charité Research Organisation GmbH

Study record dates

Study Major Dates

• Study Start (Actual): December 17, 2025
• Primary Completion (Actual): March 31, 2026
• Study Completion (Actual): March 31, 2026

Study Registration Dates

• First Submitted: December 11, 2025
• First Submitted That Met QC Criteria: January 2, 2026
• First Posted (Actual): January 13, 2026

Study Record Updates

• Last Update Posted (Actual): April 8, 2026
• Last Update Submitted That Met QC Criteria: April 7, 2026
• Last Verified: April 1, 2026

More Information

Terms related to this study

• Keywords: Obesity; Overweight; Phase 1; Pharmacokinetic; Safety and tolerability; ZP8396
• Additional Relevant MeSH Terms: Nutrition Disorders; Overnutrition; Body Weight; Pathological Conditions, Signs and Symptoms; Nutritional and Metabolic Diseases; Signs and Symptoms; Overweight; Obesity
• Other Study ID Numbers: ZP8396-25041

Plan for Individual participant data (IPD)

• Plan to Share Individual Participant Data (IPD)?: NO

Drug and device information, study documents

• Studies a U.S. FDA-regulated drug product: No
• Studies a U.S. FDA-regulated device product: No