Lysinspesifikk demetylase 1 og saltfølsomhet hos mennesker
The purpose of this study is to investigate the role dietary salt plays in epigenetic regulation of blood pressure, focusing on the salt-sensitive regulatory enzyme Lysine-specific demethylase 1.
Studieoversikt
Status
Forhold
Intervensjon / Behandling
Detaljert beskrivelse
The purpose of this study is to investigate the role dietary salt plays in epigenetic regulation of blood pressure in African American and Caucasians, focusing on the salt-sensitive regulatory enzyme Lysine-specific demethylase 1. This might help us understand why some people develop high blood pressure.
Healthy volunteers will be screened for eligibility and invited to participate in a 2 weeks study. Week 1 will be consumption of a low salt diet. Week 2 will be a high salt diet.
At the end of each week, participants will be admitted to a Clinical Research Center overnight and for one day.
On the CRC, participants will remain fasting and supine overnight and then next morning undergo hormonal and vascular testing. This will consist of blood drawing, echocardiogram, vascular tonometry, and assessment of renal blood flow before and after a low-dose Angiotensin II infusion.
The study outcome will compare how variants in the LSD1 gene affect hormonal and vascular responses according to race. This information will help us determine why some races and genetic profiles are more susceptible to detrimental effects of salt in the diet while others are protected against these effects.
Studietype
Registrering (Antatt)
Fase
- Ikke aktuelt
Kontakter og plasseringer
Studiesteder
-
-
Massachusetts
-
Boston, Massachusetts, Forente stater, 02115
- Brigham and Women's Hospital
-
-
Deltakelseskriterier
Kvalifikasjonskriterier
Alder som er kvalifisert for studier
Tar imot friske frivillige
Kjønn som er kvalifisert for studier
Beskrivelse
Inclusion Criteria:
- Age 25-45 years
- Caucasian or African American
- No gender preference (anticipate 50% female)
- Normotensive (screening blood pressure <140/90 mmHg)
- No history of hypertension, diabetes, stroke, coronary artery disease, kidney disease, cancer, thyroid disease, preeclampsia, or hospitalizations in 6 months
- Normal screening laboratory values (CMP, TSH, A1c)
- Normal ECG
- BMI <25 kg/m2
Exclusion Criteria:
- Pregnancy
- Breast feeding
- Any medication or herbal preparation
- >6oz alcohol/week
- Tobacco use
- Illicit drug use
- Chronic NSAID use
- Recent steroid use (injected, inhaled, oral)
- Decongestant use in the past 2 weeks
- Known sensitivity to infused Angiotensin II or para-amino hippurate
Studieplan
Hvordan er studiet utformet?
Designdetaljer
- Primært formål: Annen
- Tildeling: Ikke-randomisert
- Intervensjonsmodell: Parallell tildeling
- Masking: Ingen (Open Label)
Våpen og intervensjoner
Deltakergruppe / Arm |
Intervensjon / Behandling |
|---|---|
|
Aktiv komparator: Afr-Amer risk allele
African Americans carrying the LSD1 affected allele |
Change in aldosterone from baseline to after Ang II infusion on a LS diet Change in renal blood flow: High salt to low salt diet Change in vascular stiffness, baseline compared to AngII |
|
Placebo komparator: Cauc risk allele
Caucasians carrying the LSD1 affected allele |
Change in aldosterone from baseline to after Ang II infusion on a LS diet Change in renal blood flow: High salt to low salt diet Change in vascular stiffness, baseline compared to AngII |
|
Placebo komparator: Afr-Amer non-risk allele
African Americans carrying the LSD1 non-risk allele |
Change in aldosterone from baseline to after Ang II infusion on a LS diet Change in renal blood flow: High salt to low salt diet Change in vascular stiffness, baseline compared to AngII |
|
Placebo komparator: Cauc non-risk allele
Caucasians carrying the LSD1 non-risk allele |
Change in aldosterone from baseline to after Ang II infusion on a LS diet Change in renal blood flow: High salt to low salt diet Change in vascular stiffness, baseline compared to AngII |
Hva måler studien?
Primære resultatmål
Resultatmål |
Tiltaksbeskrivelse |
Tidsramme |
|---|---|---|
|
Aldosterone response to angiotensin II
Tidsramme: After 1 week dietary salt manipulation
|
Change in aldosterone, baseline to after angiotensin II by genotype/race |
After 1 week dietary salt manipulation
|
Sekundære resultatmål
Resultatmål |
Tiltaksbeskrivelse |
Tidsramme |
|---|---|---|
|
Renal blood flow response to dietary salt
Tidsramme: After 1 week dietary salt manipulation on both diets
|
Change in renal blood flow, high salt diet to low salt diet by genotype/race |
After 1 week dietary salt manipulation on both diets
|
|
Vascular stiffness response to angiotensin II
Tidsramme: After 1 week dietary salt manipulation
|
Change in vascular stiffness, baseline to after angiotensin II by genotype/race |
After 1 week dietary salt manipulation
|
Samarbeidspartnere og etterforskere
Sponsor
Publikasjoner og nyttige lenker
Generelle publikasjoner
- Krug AW, Tille E, Sun B, Pojoga L, Williams J, Chamarthi B, Lichtman AH, Hopkins PN, Adler GK, Williams GH. Lysine-specific demethylase-1 modifies the age effect on blood pressure sensitivity to dietary salt intake. Age (Dordr). 2013 Oct;35(5):1809-20. doi: 10.1007/s11357-012-9480-0. Epub 2012 Oct 2.
- Williams JS, Chamarthi B, Goodarzi MO, Pojoga LH, Sun B, Garza AE, Raby BA, Adler GK, Hopkins PN, Brown NJ, Jeunemaitre X, Ferri C, Fang R, Leonor T, Cui J, Guo X, Taylor KD, Ida Chen YD, Xiang A, Raffel LJ, Buchanan TA, Rotter JI, Williams GH, Shi Y. Lysine-specific demethylase 1: an epigenetic regulator of salt-sensitive hypertension. Am J Hypertens. 2012 Jul;25(7):812-7. doi: 10.1038/ajh.2012.43. Epub 2012 Apr 26.
- Pojoga LH, Williams JS, Yao TM, Kumar A, Raffetto JD, do Nascimento GR, Reslan OM, Adler GK, Williams GH, Shi Y, Khalil RA. Histone demethylase LSD1 deficiency during high-salt diet is associated with enhanced vascular contraction, altered NO-cGMP relaxation pathway, and hypertension. Am J Physiol Heart Circ Physiol. 2011 Nov;301(5):H1862-71. doi: 10.1152/ajpheart.00513.2011. Epub 2011 Aug 26.
- Heydarpour M, Parksook WW, Pojoga LH, Williams GH, Williams JS. Mineralocorticoid Receptor and Aldosterone: Interaction Between NR3C2 Genetic Variants, Sex, and Age in a Mixed Cohort. J Clin Endocrinol Metab. 2024 Dec 18;110(1):e140-e149. doi: 10.1210/clinem/dgae127.
Studierekorddatoer
Studer hoveddatoer
Studiestart (Faktiske)
Primær fullføring (Faktiske)
Studiet fullført (Antatt)
Datoer for studieregistrering
Først innsendt
Først innsendt som oppfylte QC-kriteriene
Først lagt ut (Antatt)
Oppdateringer av studieposter
Sist oppdatering lagt ut (Faktiske)
Siste oppdatering sendt inn som oppfylte QC-kriteriene
Sist bekreftet
Mer informasjon
Begreper knyttet til denne studien
Ytterligere relevante MeSH-vilkår
Andre studie-ID-numre
- 2015P002540
Plan for individuelle deltakerdata (IPD)
Planlegger du å dele individuelle deltakerdata (IPD)?
IPD-planbeskrivelse
IPD will be shared first thru the publication of research results in peer-review scientific journals by the study investigator. IPD will be made available upon reasonable request from bona fide research organizations in an effort to provide broader impact and enrich public health knowledge. Research volunteer PHI will not be shared, and all participant data will be de-identified and coded. Only encrypted and secure network exchanges will be used for data transfer. Cost sharing my be required in order prepare datasets.
Legemiddel- og utstyrsinformasjon, studiedokumenter
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