Leniolisibe para desregulação imunológica em CVID

Inclusion Criteria:

1. Subject is 12 to 75 years of age (inclusive).

   a. Specifically at sites located in the United Kingdom subjects must be 18 to 75 years of age (inclusive)

2. Subject must have a minimum body weight of 45 kg

3. Subject has a clinical diagnosis of CVID supported by all of the following (a thru c):

   1. A low IgG level compared to age-adjusted reference range [OR if this cannot be documented, subject must have one of the following: i) absent isohemagglutinins and/or poor response to vaccines; or ii) Low class-switched memory B cells less than 2%]
   2. Low IgA and/or IgM compared to age-adjusted reference range
   3. No identified secondary causes of hypogammaglobulinemia

4. Inborn Errors of Immunity/ PID Panel testing:

   1. Lacks an identified pathogenic/likely pathogenic genetic driver for their CVID primary immunodeficiency OR
   2. Subject has an identified pathogenic/likely pathogenic genetic driver(s) for their CVID limited to the following genes: TNFRSF13B (TACI), TNFRSF13C (BAFFR), CD19, CD20, CD81, CR2 (CD21), LRBA, CTLA4, NFKB1, NFKB2, IKZF1 (excluding variants associated with combined immune deficiency), CARD11 (gain of function), SH3KBP1, SEC61A1 IRF2BP2, CTNNBL1, TWEAK or PTEN.

5. Subject has lymphoproliferation, as evidenced by CT imaging: splenomegaly with craniocaudal spleen measurement >10 cm and/or lymphadenopathy with at least 1 measurable index lymph node (long axis >1.5 cm) as per Cheson methodology.

6. Subject has at least ONE of the following CVID clinical manifestations of immune dysregulation:

   1. Clinical symptoms related to splenomegaly or lymphadenopathy which interfere with activities of daily living or are associated with chronic pain, dyspnea, functional impairment, or limitations in usual activities
   2. One or more blood cytopenias related to CVID (and not due to other medical conditions such as iron-deficiency or lead exposure) defined as hemoglobin <10 g/dL, platelet count <100,000/µL, and/or neutrophil count <1,000/µL
   3. Previous pathologic confirmation of ILD and attributed to CVID by the Investigator with quantifiable CT chest imaging findings evident on baseline CT scan
   4. Clinical diagnosis of CVID enteropathy or other GI tract diagnosis attributable to CVID by the Investigator which involves the small intestine and meets the following enteropathy criteria:

   i. Clinical symptoms of GI disease including at least 1 of: abdominal pain or diarrhea at least 3 days of the week for at least 4 weeks or longer, or dependence on supplemental enteral or parenteral nutrition ii. Lacks a clinical diagnosis of Celiac Disease, and negative human leukocyte antigen (HLA)-DQ2 and -DQ8 testing at screening iii. Presence of small bowel villous shortening/atrophy/blunting with or without intraepithelial lymphocytosis noted in a pathology report pertaining to a small bowel biopsy performed within 5 years of enrollment iv. Negative stool PCR Gastrointestinal Profile at screening

7. At screening, vital signs. Ranges:

   Systolic blood pressure 80-159 mm Hg Diastolic blood pressure 50-109 mm Hg Pulse rate 50-110 beats per minute (bpm) Oxygen saturation 93-100%

8. Subjects or their legal representatives (for subjects under the age of 18 years) must be able to communicate with the Investigator and understand and comply with the requirements of the study, including an ability to provide written informed consent.

Exclusion Criteria:

1. Laboratory evidence of significant T cell deficiency including CD4+ T cells <200/uL.

2. Laboratory evidence of significant NK cell deficiency including NK cells <1% of peripheral blood lymphocytes or less than 50/mcL.

3. Clinical history of infections suggestive of clinically significant T cell or NK cell deficiency such as Pneumocystis jirovecii, atypical mycobacteria, severe warts, or unusually severe (as determined by the PI) infections with herpesviruses.

4. Presence of uncontrolled chronic/recurrent infectious disease (except those considered to be characteristic of antibody deficiency).

5. Positive blood polymerase chain reaction (PCR) for cytomegalovirus.

6. Evidence of tuberculosis infection

7. Positive blood cryptococcal antigen

8. Previous or concurrent use of immunosuppressive medication, such as:

   • Use of an mTOR inhibitor or a PI3K inhibitor within 3 weeks.
   • Rituximab or other B cell depleting antibodies, belimumab, cyclophosphamide, or alemtuzumab within 6 months.
   • Cyclosporine A, mycophenolate mofetil, 6-mercaptopurine, azathioprine, methotrexate, tacrolimus, ruxolitinib, or other Janus kinase (JAK) inhibitors within 3 weeks.
   • Glucocorticoids above 25 mg prednisone or equivalent per day within 2 weeks
   • Immunosuppressive monoclonal or polyclonal antibody therapeutics such as directed against TNF-alpha, α₄β₇ integrin, IL-6, IL-12/IL-23 and others within 5 half-lives
   • Other immunosuppressive agents expected to have a significant impact on immune cell number or function.
   • Abatacept is allowed during study if the subject has been receiving a stable dosing regimen for more than 3 months .
   • Enteral budesonide is allowed during study if the subject has been receiving a stable dosing regimen for more than 3 months.

9. Subject is receiving concurrent treatment with another investigational therapy or use of another investigational therapy less than 4 weeks or 5 half-lives (whichever is longer).

10. HIstory of hypersensitivity to the study drug or to drugs of similar chemical classes.

11. Current use of medication known to be a strong inhibitor, or moderate or strong inducer, of isoenzyme cytochrome P450 CYP3A.

12. Current use of medications that, to a larger extent, are BCRP, OATP1B1, and/or OATP1B3 substrates.

13. History of HIV or positive test result at screening.

14. Any surgical or medical condition which may jeopardize the subject in case of participation in the study.

    • Chronic need for supplemental oxygen or invasive or non-invasive respiratory support.
    • Liver failure or clinically significant liver disease or dysfunction as indicated by alanine transaminase (ALT) or aspartate transaminase (AST) greater than 2.5 times the upper limit of normal, bilirubin greater than 1.5 times the upper limit of normal, international normalized ratio (INR) greater than 1.5 in the absence of anticoagulation, or presence of diuretic refractory ascites.
    • Elevation of ALT or AST up to 6 times the upper limit of normal is allowed if elevation is determined to be a consequence of immune dysregulation by the site Principal Investigator and if the elevation has been stable for 3 months prior to enrollment.
    • History of significant renal injury/renal disease severely affecting renal function or presence of impaired renal function as indicated by estimated glomerular filtration rate of less than 30 mL/min/1.73 m2.

15. A positive hepatitis B surface antigen (HBsAg), positive hepatitis B PCR, positive hepatitis C PCR, or positive hepatitis C antibody result at screening.

16. Administration of live vaccines starting from 6 weeks before first dose of study medication

17. Subject has a history of malignancy (except lymphoma) within 3 years before the first dose of study medication or has evidence of residual disease from a previously diagnosed malignancy, except for adequately treated cancers of the skin (basal or squamous cell) or carcinoma in situ of the uterine cervix.

18. Subject has a previous diagnosis of lymphoma that has been treated with chemotherapy, radiotherapy, or transplant within 1 year of the first dose of study medication or is anticipated to require lymphoma treatment within 6 months of the first dose of study medication.

19. Subject has uncontrolled post-transplant lymphoproliferative disease-like Epstein-Barr virus related lymphoproliferative disease.

20. Donation or loss of 400mL or more of blood within 8 weeks before the first dose of study medication

21. Subject has had major surgery requiring hospitalization or radiotherapy within 4 weeks prior to the first dose of study medication or has a planned or expected major surgical procedure during the study period.

22. Pregnant or nursing (lactating) women.

23. Individuals of child-bearing potential, unless they are using highly effective methods of contraception