Follicular Fluid, Bioavailable Vitamin D and Embryo Quality Markers

Inclusion Criteria:

• Women´s Age >18 - ≤43 years.
• BMI 19-33
• Male partner will produce fresh sperm sample on the same day of the egg collection procedure.
• Women with more than 10 follicles at the time of the trigger
• At the time of egg retrieval, at least three follicles between 14-<17 mm and three follicles ≥17 mm.
• Women ≤35 years old stimulation with rFSH antagonist
• Women ≥36 years old stimulation with highly-purified human menopausal gonadotropins (hpHMG) specifically for the additional LH.
• Middle East population

Exclusion Criteria:

• Polycystic ovarian syndrome (according to the Rotterdam Criteria).
• Patients at risk of OHSS, according to the criteria previously described by Papanikolau et al. (23).
• History of endometriosis, classified according to the American Fertility Society (AFS) as stage 3 or more.
• Severe male factor definition (<2 million of sperm per ml of ejaculate)
• Recurrent miscarriage (3 miscarriages)
• Smokers
• History of chemotherapy or radiotherapy