Study to Assess the Effect Of Alosetron On Mucosal Blood Flow

A Randomize, Placebo-controlled, Crossover Study to Measure the Effect of Alosetron on Mucosal Blood Flow in Female Healthy Volunteers and Diarrhea-predominant IBS Subjects

This study will look at colonic mucosal blood flow in subjects who have taken alosetron vs placebo and healthy volunteers vs diarrhea-predominant Irritable Bowel Syndrome (d-IBS) patients.

Study Overview

• Status: Completed
• Conditions: Irritable Colon
• Intervention / Treatment: Drug: alosetron

• Study Type: Interventional
• Enrollment (Actual): 49
• Phase: Phase 4

Contacts and Locations

Study Locations

• United Kingdom
  • London, United Kingdom, W1G 8HU
    • GSK Investigational Site

Participation Criteria

Eligibility Criteria

• Ages Eligible for Study: 18 years to 49 years (ADULT)
• Accepts Healthy Volunteers: Yes
• Genders Eligible for Study: Female

Description

Inclusion criteria:

• The subject signs and dates a written informed consent form prior to the initiation of any study-related activities.
• The subject is between 18 and 49 years of age at the time of the Screening Visit.

• The subject is female and either:

  • A healthy subject. Healthy subjects are defined as individuals who are free from clinically significant illness or disease as determined by their medical history (including family), physical examination, laboratory studies, and other tests.

OR

• A d-IBS patient per the Rome II criteria who has a normal result from a flexible sigmoidoscopy or colonoscopy, or flexible sigmoidoscopy plus barium enema, within 2 years of the Screening visit.

  • The subject demonstrates a negative urine pregnancy test result prior to investigational product administration and be either:
• Of non-childbearing potential (i.e., physiologically incapable of becoming pregnant)
• post-menopausal define as one year without menses in the absence of hormone replacement therapy.
• sterilization (via hysterectomy or bilateral tubal ligation)
• Of childbearing potential and agrees to one of the following acceptable non-hormonal contraceptive methods consistently and in accordance with both the product label and the instructions of a physician. Subjects will use effective contraceptive methods for at least one month prior to Screening and should continue to use the same contraceptive method throughout the study (Follow-up Visit).
• Complete abstinence from intercourse
• an intra-uterine device (IUD) inserted by a qualified physician, provided the IUD is not of the hormonal type and it has published data showing that the highest expected failure rate is less than 1% per year (not all IUDs meet this criterion)
• double barrier method if comprised of a spermicide with either a condom or diaphragm
• sterilization of partner The subject is ambulatory (defined as not depending exclusively on a wheelchair for mobility).

Exclusion criteria:

• The subject is taking oral contraceptive or other hormonal therapy.
• The subject has a concurrent illness or disability that may affect the interpretation of clinical data, or otherwise contraindicates participation in this clinical study (e.g., an unstable cardiovascular, autoimmune, renal, hepatic, pulmonary, endocrine, metabolic, gastrointestinal, hematologic, or neurological condition).
• The subject has constipation-predominant IBS (c-IBS) or alternating IBS per the ROME II criteria.
• The subject has current evidence of or history of chronic or severe constipation, or a history of sequelae from constipation.

• Evidence of a biochemical or structural abnormality of the digestive tract. These conditions include (but not limited to):

  • Current evidence, or history of (at any time in the past):
  • GI/Bowel conditions:
  • inflammatory bowel disease (Crohn's disease or ulcerative colitis)
  • celiac disease
  • laxative abuse (in the clinical judgement of the physician)
  • gastrointestinal surgery (exceptions include ≥6 months post-surgery appendectomy, cholecystectomy, fundoplication without gas bloat, or hiatal hernia repair; ≥3 months post-surgery herniorrhaphy without bowel resection)
  • gastroparesis
  • GI malignancy
  • carcinoid syndrome
  • amyloidosis
  • gastrointestinal adhesions
  • ischemic colitis
  • toxic megacolon
  • impaired intestinal circulation
  • gastrointestinal perforation
  • gastrointestinal obstruction and/or stricture
  • Ischemic cardiovascular conditions:
  • coronary artery disease (CAD)
  • significant atherosclerosis
  • chronic pancreatitis
  • diabetes
  • thrombophlebitis or hypercoagulable state.
  • Current evidence of (within the past 6 months):
  • diverticulitis
  • ileus
  • symptomatic cholelithiasis
  • proctitis.
  • Current evidence of:
  • Hemoccult (+) stool.
• The subject has a BMI of ≥27.
• Mental impairment or inability or refusal to follow directions.
• The subject has current evidence of, or has been treated for a malignancy within the past five years (other than localized basal cell, squamous cell skin cancer or cancer in situ that has been resected).
• The subject exhibits evidence of hepatic dysfunction, viral hepatitis, or exhibits serum ALT (alanine aminotransferase) (SGPT), AST (aspartate aminotransferase) (SGOT) values >2.5 times the upper limit of normal or alkaline phosphatase or bilirubin values >2.0 times the upper limit of normal.
• The subject displays renal impairment as evidenced by a serum creatinine value >2.0 mg/dl.
• The subject has used any medication within the seven days prior to dosing, unless approved by the investigator and GlaxoSmithKline (GSK) personnel. Section 9.1.
• The subject has used an investigational drug, or participated in an investigational study, within 30 days of the Screening Visit.
• The subject has a history of drug allergies (including but not limited to hypersensitivity responses to alosetron which, in the opinion of the investigator, contraindicates the subject's participation in this study.
• Subjects who have made a blood donation (>450mL) within 6 weeks prior to screening.
• The subject has a history of alcohol and/or substance abuse within the past two years.
• The subject is pregnant. The subject is breastfeeding.

Study Plan

How is the study designed?

Design Details

• Primary Purpose: TREATMENT
• Allocation: RANDOMIZED
• Interventional Model: CROSSOVER
• Masking: DOUBLE

What is the study measuring?

Primary Outcome Measures

Outcome Measure	Measure Description	Time Frame
Left Colon Mucosal Blood Flow (MBF)	On Day 6 of each treatment period; 1 hour after dosing, subjects underwent a flexible sigmoidoscopy with Laser Doppler Flowmetry (LDF) to measure Mucosal Blood Flow (MBF). There were no pre-treatment LDF procedure, MBF was compared between the Healthy volunteers and D-irritable bowel syndrome (IBS) cohorts using the flow rates from the placebo treatment period.	Day 6 after each treatment period

Secondary Outcome Measures

Outcome Measure	Measure Description	Time Frame
Rectal Mucosal Blood Flow (MBF)	On Day 6 of each treatment period approximately 1 hour after dosing, subjects underwent a flexible sigmoidoscopy with Laser Doppler Flowmetry (LDF) to measure Mucosal Blood Flow (MBF). There was no pre-treatment LDF procedure, MBF was compared between the Healthy and d-IBS cohorts using the flow rates from the placebo treatment period.	Day 6 after each treatment period
Left Colon and Rectal Mucosal Blood Flow Cohort Comparisons	On Day 6 of each treatment period approximately 1 hour after dosing, subjects underwent a flexible sigmoidoscopy with Laser Doppler Flowmetry (LDF) to measure Mucosal Blood Flow (MBF). There was no pre-treatment LDF procedure, MBF was compared between the Healthy and d-IBS cohorts using the flow rates from the placebo treatment period.	Day 6 after each treatment period

Collaborators and Investigators

• Sponsor: GlaxoSmithKline

Study record dates

Study Major Dates

• Study Start: December 1, 2006
• Primary Completion (ACTUAL): December 1, 2007
• Study Completion (ACTUAL): December 1, 2007

Study Registration Dates

• First Submitted: August 28, 2006
• First Submitted That Met QC Criteria: August 28, 2006
• First Posted (ESTIMATE): August 30, 2006

Study Record Updates

• Last Update Posted (ESTIMATE): May 18, 2015
• Last Update Submitted That Met QC Criteria: May 15, 2015
• Last Verified: May 1, 2015

More Information

Terms related to this study

• Keywords: mucosal blood flow d-IBS
• Additional Relevant MeSH Terms: Digestive System Diseases; Gastrointestinal Diseases; Colonic Diseases, Functional; Colonic Diseases; Intestinal Diseases; Irritable Bowel Syndrome; Physiological Effects of Drugs; Neurotransmitter Agents; Molecular Mechanisms of Pharmacological Action; Gastrointestinal Agents; Serotonin Agents; Serotonin Antagonists; Alosetron
• Other Study ID Numbers: S3B40042