- ICH GCP
- US Clinical Trials Registry
- Clinical Trial NCT01347905
Changes in Iron Absorption and Availability Before and After Weight Loss in Obese Pre-menopausal Women and Men.
Study Overview
Detailed Description
Until now adiposity has been associated with low iron status and with higher hepcidin levels. Moreover, weight loss and thereby loss of fat mass, has been associated with decreased hepcidin levels and improved iron status. However, until now no direct measures of iron absorption or incorporation into erythrocytes have been assessed before and after losing weight/fat mass.
We hypothesize that:
- body fat loss in obese women and men will improve iron absorption, corrected for iron status, from a labeled test meal measured by using a stable isotopes technique by on average 30%.
- body fat loss in obese women and men will decrease the related inflammatory state.
- greater subclinical inflammation in obese women and men will be correlated with a decreased incorporation of iron into erythrocytes, and that this will improve after loss of body fat.
The study is a prospective cohort in which iron bioavailability will be assessed in obese women and men before and after weight loss and associated loss of body fat over a period of 6 months.
Iron absorption will be estimated using stable-isotope techniques where incorporation of 57Fe and 58Fe into erythrocytes is measured 14 days after administration. Preparation of isotopically labelled iron will be done at the Laboratory of Human Nutrition of the Swiss Federal Institute of Technology Zurich (ETH Zürich).
A baseline venous blood sample will be drawn 6-8 weeks after the surgery when surgery-related inflammation has been resolved. The subjects will receive a test drink, containing 6 mg of 57Fe labelled ferrous sulphate. One hour later, 2 ml of an aqueous solution containing 100 μg 58Fe as iron citrate in 250 cc of normal saline will be infused over 50 min. This infusion protocol has been used safely and successfully to examine iron metabolism in both adults and infants. Fourteen days later, a second venous blood sample will be drawn. This procedure will be performed at baseline (6-8 weeks post-surgery) and at the end of the study (6 months post baseline).
Our study could provide important information to establish the mechanism by which obesity-mediated inflammation could induce iron deficiency. This will be the first human trial that will evaluate if the obesity-related reduction of iron absorption is reversible in a context where obesity and iron deficiency are both highly prevalent.
Study Type
Enrollment (Actual)
Contacts and Locations
Study Locations
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Nuevo Leon
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San Pedro Garza García, Nuevo Leon, Mexico, 66238
- Universidad de Monterrey (UDEM) División de Ciencias de la Salud
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Participation Criteria
Eligibility Criteria
Ages Eligible for Study
Accepts Healthy Volunteers
Genders Eligible for Study
Sampling Method
Study Population
Description
Inclusion Criteria:
- Women and men
- aged 18 to 50 years
- Premenopausal (had no absence of menstrual cycle in the past 12 months)
- BMI ≥35<45
- Indication for laparoscopic Sleeve Gastrectomy (LSG) or Laparoscopic Adjustable Gastric Band (LAGB).
Subjects are eligible for this surgery if they:
have BMI ≥35<45 and one of the following two conditions:
- Medical, physical or psychosocial problems associated with their obesity.
- A history of prolonged attempts at weight loss by other means.
have been adequately informed and understood and accepted the potential -- risks and benefits of the procedure, and expressed a commitment to follow the rules regarding eating and exercise permanently after the procedure.
- No postoperative complications after the surgery
- Apparently healthy: no significant medical conditions that could influence iron or inflammatory status other than obesity (i.e., cancer, HIV/AIDS, inflammatory bowel disease, gastrointestinal bleeding, and rheumatoid arthritis, renal disease or hemochromatosis) (judged by study physician).
- No use of medication which interferes with study measurements (as judged by the study physician)
- Willing to not change their intake of dietary iron and not take additional iron supplements, other than provided by the study team, between baseline and study end.
- Not being pregnant during the study period or in the six months prior to the study and not planning to become pregnant until at least 3 months after the last study visit.
- Non-lactating in the 6 weeks prior to the study and for the duration of the study
- No reported weight loss ≥ 10 % of bodyweight during a period of six months before pre-surgery examination.
- No full or partial hysterectomy in the past 3 months
- Informed consent signed
- Agreeing to be informed about medically relevant personal test-results by a physician.
- Agreeing to be informed about medically relevant personal test-results by a physician.
- No reported participation in night shift work two weeks prior to pre-study investigation and during the study. Night work is defined as working between midnight and 6.00 AM.
- Consumption of =< 14 alcoholic drinks in a typical week
- Strenuous exercise =< 10 hours per week
Exclusion Criteria:
- Reported excessive blood loss at surgery (> 500 ml) and/or moderate/severe anemic (Hb < 100 g/L)
- Complications during restrictive bariatric surgery defined as either:
- Surgery duration of > 3 hours
- Blood loss of > 500 ml
- Perforation of the gastrointestinal tract
- Inability to adjust lapband
- Women and men with diagnosed abnormalities in iron metabolism (diagnosed from routine pre-surgery blood sample)
Study Plan
How is the study designed?
Design Details
- Observational Models: Cohort
- Time Perspectives: Prospective
Cohorts and Interventions
Group / Cohort |
Intervention / Treatment |
---|---|
Obese women and men
Obese women and men undergoing restrictive bariatric surgery.
Iron absorption will be estimated using stable-isotope techniques where incorporation of 57Fe and 58Fe into erythrocytes is measured 14 days after administration.
This procedure will be performed at baseline (6 weeks post-surgery) and at the end of the study (6-7 months post baseline).
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Iron absorption will be estimated using stable-isotope techniques where incorporation of 57Fe and 58Fe into erythrocytes is measured 14 days after administration.
The subjects will provide an initial blood sample (20ml) and then receive a test drink, containing 6 mg of 57Fe labeled ferrous sulphate.
One hour later, 2 ml of an aqueous solution containing 100 μg 58Fe, as iron citrate in 250cc of normal saline will be infused over 50 min.
Post infusion a 4ml blood sample will be taken.
This procedure will be performed at baseline (6 weeks post-surgery) and at the end of the study (6-7 months post baseline).
Other Names:
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What is the study measuring?
Primary Outcome Measures
Outcome Measure |
Time Frame |
---|---|
Fractional iron absorption before and after weight loss, based on 57Fe and 58Fe isotope concentrations in erythrocytes.
Time Frame: 6 months
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6 months
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Secondary Outcome Measures
Outcome Measure |
Time Frame |
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Iron status and regulatory markers (Hb, serum ferritin, transferrin receptor, hepcidin),(pro)inflammatory markers (CRP, AGP, TNF-α, IL-6, leptin), blood volume, body composition (DXA).
Time Frame: 6 months
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6 months
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Collaborators and Investigators
Sponsor
Collaborators
Investigators
- Study Director: Michael B Zimmermann, MD PHD, Wageningen University
Publications and helpful links
Study record dates
Study Major Dates
Study Start
Primary Completion (Actual)
Study Completion (Actual)
Study Registration Dates
First Submitted
First Submitted That Met QC Criteria
First Posted (Estimate)
Study Record Updates
Last Update Posted (Estimate)
Last Update Submitted That Met QC Criteria
Last Verified
More Information
Terms related to this study
Additional Relevant MeSH Terms
Other Study ID Numbers
- Iron absorption and obesity
This information was retrieved directly from the website clinicaltrials.gov without any changes. If you have any requests to change, remove or update your study details, please contact register@clinicaltrials.gov. As soon as a change is implemented on clinicaltrials.gov, this will be updated automatically on our website as well.
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