Safety and Efficacy of Dapagliflozin in Triple Therapy to Treat Subjects With Type 2 Diabetes

A Multicenter, Randomized, Double-Blind, Placebo-Controlled, Parallel Group, Phase 3 Trial to Evaluate the Safety and Efficacy of Therapy With Dapagliflozin Added to Saxagliptin in Combination With Metformin Compared to Therapy With Placebo Added to Saxagliptin in Combination With Metformin in Subjects With Type 2 Diabetes Who Have Inadequate Glycemic Control on Metformin and Saxagliptin

The purpose of this study is to learn if BMS-512148 (Dapagliflozin) as part of a triple combination therapy can improve (decrease) hemoglobin A1c in patients with type 2 diabetes after 24 weeks of treatment compared to a 2 drug oral antidiabetic therapy. The safety of this treatment will also be studied.

Study Overview

• Status: Completed
• Conditions: Type 2 Diabetes
• Intervention / Treatment: Drug: Dapagliflozin; Drug: Saxagliptin; Drug: Metformin immediate release (IR); Drug: Placebo matching with Dapagliflozin

Detailed Description

Prior to randomization, all eligible subjects will receive open-label treatment with Saxagliptin 5mg and Metformin IR during the 16-week open-label treatment period.

• Study Type: Interventional
• Enrollment (Actual): 320
• Phase: Phase 3

Contacts and Locations

Study Locations

• Czech Republic
  • Broumov, Czech Republic, 550 01
    • Local Institution
  • Pardubice, Czech Republic, 530 02
    • Local Institution
  • Praha 10, Czech Republic, 100 00
    • Local Institution
  • Praha 4, Czech Republic, 149 00
    • Local Institution
  • Pribram V, Czech Republic, 261 95
    • Local Institution
• Mexico
  • Aguascalientes, Mexico, 20127
    • Local Institution
  • Jalisco
    • Guadalajara, Jalisco, Mexico, 44670
      • Local Institution
    • Zapopan, Jalisco, Mexico, 45200
      • Local Institution
    • Zapopan, Jalisco, Mexico, 45116
      • Local Institution
  • Nuevo Leon
    • Monterrey, Nuevo Leon, Mexico, 64460
      • Local Institution
    • Monterrey, Nuevo Leon, Mexico, 64060
      • Local Institution
• Poland
  • Bialystok, Poland, 15-435
    • Local Institution
  • Krakow, Poland, 30-015
    • Local Institution
  • Ruda Slaska, Poland, 41-709
    • Local Institution
  • Warszawa, Poland, 01-868
    • Local Institution
  • Warszawa, Poland, 00-465
    • Local Institution
  • Warszawa, Poland, 03-003
    • Local Institution
  • Zory, Poland, 44-240
    • Local Institution
• Puerto Rico
  • San Juan, Puerto Rico, 00909
    • Clinical Research Puerto Rico
• Romania
  • Bucharest, Romania, 010825
    • Local Institution
  • Bucharest, Romania, 070208
    • Local Institution
  • Constanta, Romania, 900591
    • Local Institution
  • Craiova, Romania, 200349
    • Local Institution
  • Galati, Romania, 800098
    • Local Institution
  • Ploiesti, Romania, 100097
    • Local Institution
• Russian Federation
  • Kursk, Russian Federation, 305035
    • Local Institution
  • Moscow, Russian Federation, 119034
    • Local Institution
  • Saint-petersburg, Russian Federation, 194044
    • Local Institution
  • St. Petersburg, Russian Federation, 195257
    • Local Institution
  • St. Petersburg, Russian Federation, 194044
    • Local Institution
  • St. Petersburg, Russian Federation, 194156
    • Local Institution
  • St. Petersburg, Russian Federation, 197136
    • Local Institution
  • St.petersburg, Russian Federation, 195112
    • Local Institution
  • St.petersburg, Russian Federation, 197022
    • Local Institution
  • Yaroslaval, Russian Federation, 150062
    • Local Institution
• United Kingdom
  • Bedfordshire, United Kingdom, SG19 3JR
    • Local Institution
  • London, United Kingdom, W6 7HY
    • Local Institution
  • Hants
    • Portsmouth, Hants, United Kingdom, PO3 6LY
      • Local Institution
  • Isle of Wight
    • Newport, Isle of Wight, United Kingdom, PO30 5TG
      • Local Institution
  • Merseyside
    • Liverpool, Merseyside, United Kingdom, L7 8XP
      • Local Institution
  • Wiltshire
    • Chippenham, Wiltshire, United Kingdom, SN15 1HP
      • Local Institution
• United States
  • Alabama
    • Birmingham, Alabama, United States, 35294
      • University of Alabama at Birmingham
  • Arizona
    • Mesa, Arizona, United States, 85213
      • Clinical Research Advantage Inc/Desert Clinical Research Llc
    • Phoenix, Arizona, United States, 85018
      • Elite Clinical Studies, LLC
    • Phoenix, Arizona, United States, 85018
      • Clinical Research Advantage, Inc.
  • Arkansas
    • Little Rock, Arkansas, United States, 72205
      • Arkansas Clinical Research
  • California
    • Lomita, California, United States, 90717
      • Torrance Clinical Research Institute Inc.
    • Los Angeles, California, United States, 90057
      • National Research Institute
    • Los Angeles, California, United States, 90023
      • Randall G. Shue, Do, Inc.
    • Northridge, California, United States, 91325
      • Diabetes Medical Center Of California
    • Vista, California, United States, 92083
      • Cassidy Medical Group/Clinical Research Advantage
  • Florida
    • Hialeah, Florida, United States, 33012
      • Palm Springs Research Institute
    • Kissimmee, Florida, United States, 34741
      • FPA Clinical Research
    • Miami, Florida, United States, 33183
      • International Research Associates, LLC
    • Orlando, Florida, United States, 32804
      • Omega Research Consultants, LLC
    • Oviedo, Florida, United States, 32765
      • Compass Research East, LLC
    • Palm Harbor, Florida, United States, 34684
      • Palm Harbor Medical Associates
  • Illinois
    • Chicago, Illinois, United States, 60607
      • Cedar Crosse Research Center
  • Indiana
    • Evansville, Indiana, United States, 47714
      • Clinical Research Advantage
  • Michigan
    • Battle Creek, Michigan, United States, 49015
      • Associated Internal Medicine Specialists
  • Mississippi
    • Rolling Fork, Mississippi, United States, 39159
      • Jackson Clinic
  • New Jersey
    • Trenton, New Jersey, United States, 08611
      • Premier Research
  • North Carolina
    • Charlotte, North Carolina, United States, 28204
      • Metrolina Internal Medicine
  • Ohio
    • Cincinnati, Ohio, United States, 45246
      • Sterling Research Grp, Ltd.
  • Texas
    • Houston, Texas, United States, 77004
      • Endocrine Associates
    • San Antonio, Texas, United States, 78229
      • Sam Clinical Research Center
  • Virginia
    • Virginia Beach, Virginia, United States, 23454
      • Tidewater Integrated Medical Research

Participation Criteria

Eligibility Criteria

• Ages Eligible for Study: 18 years and older (Adult, Older Adult)
• Accepts Healthy Volunteers: No
• Genders Eligible for Study: All

Description

Inclusion Criteria

1. Signed Written Informed Consent

   1. Subjects must be willing and able to give signed and dated written informed consent.

2. Target Population

   For inclusion into Stratum A:

   i) Subjects with T2DM with inadequate glycemic control, defined as central laboratory HbA1c ≥ 8.0 and ≤ 11.5% obtained at the screening visit (ie Week -18 visit), on stable metformin therapy alone for at least 8 weeks prior to screening visit at a dose ≥ 1500 mg per day

   For inclusion into Stratum B:

   ii) Subjects with T2DM with inadequate glycemic control, and HbA1c ≥ 7.5 and ≤ 10.5% obtained at the screening visit and on stable metformin therapy at a dose ≥ 1500 mg per day AND a DPP4 inhibitor at the maximum approved dose for at least 8 weeks prior to screening visit.

   b) C-peptide ≥ 1.0 ng/mL (0.34 nmol/L) at screening visit. c) BMI ≤ 45.0 kg/m2 at the screening visit.

3. Age and Reproductive Status

   1. Men and women, aged ≥ 18 years old at time of screening visit.
   2. Women of childbearing potential (WOCBP) must be using an acceptable method of contraception to avoid pregnancy throughout the study in such a manner that the risk of pregnancy is minimized.
   3. WOCBP must have a negative serum or urine pregnancy test within 24 hours prior to the start of investigational product.
   4. Women must not be breastfeeding
   5. Sexually active fertile men must use highly effective birth control if their partners are WOCBP.

Exclusion Criteria

1. Target Disease Exceptions

   1. History of diabetes insipidus
   2. Symptoms of poorly controlled diabetes that would preclude participation in this trial including but not limited to marked polyuria and polydipsia with greater than 10% weight loss during the three months prior to screening, or other signs and symptoms.
   3. History of diabetic ketoacidosis or hyperosmolar nonketotic coma.

2. Medical History and Concurrent Diseases

   1. History of bariatric surgery or lap-band procedure within 12 months prior to screening.
   2. Any unstable endocrine, psychiatric or rheumatic disorders as judged by the Investigator.
   3. Subject who, in the judgment of the investigator, may be at risk for dehydration or volume depletion that may affect the interpretation of efficacy or safety data and concomitant use of loop diuretics in countries where this is not recognized in the Dapagliflozin label.

   4. Subject is currently abusing alcohol or other drugs or has done so within the last 6 months.

      Acute Vascular Event:

   5. Uncontrolled hypertension defined as systolic blood pressure (SBP) ≥ 160 mmHg and/or diastolic blood pressure (DBP) ≥ 100 mmHg.
   6. Cardiovascular Disease within 3 months of the screening visit [ie myocardial infarction, cardiac surgery or revascularization (CABG/PTCA), unstable angina, stroke or transient ischemic attack (TIA)].

   7. Congestive heart failure as New York Association (NYHA) class IV, unstable or acute congestive heart failure.

      Renal Diseases:

   8. Moderate or severe impairment of renal function [defined as eGFR < 60 mL/min/1.73m2 (estimated by MDRD) or serum creatinine (Scr) ≥ 1.5 mg/dL in males or ≥ 1.4 mg/dL in females.]

   9. Conditions of congenital renal glucosuria

      Hepatic Diseases:

   10. Significant hepatic disease, including, but not limited to, chronic active hepatitis and/or severe hepatic insufficiency, including subjects with ALT and/or AST > 3x ULN and or Total Bilirubin > 2.5 x ULN.

       Hematological and Oncological Disease/Conditions:

   11. History of hemoglobinopathy, with the exception of sickle cell trait (SA) or thalassemia minor; or chronic or recurrent hemolysis.
   12. Malignancy within 5 years of the screening visit (with the exception of treated basal cell or treated squamous cell carcinoma)
   13. Known immunocompromised status, including but not limited to, individuals who have undergone organ transplantation or who are positive for the human immunodeficiency virus.

   14. Donation of blood or blood products to a blood bank, blood transfusion, or participation in a clinical study requiring withdrawal of > 400 mL of blood during the 6 months prior to the screening visit.

       Prohibited treatment and therapies:

   15. Administration of any antihyperglycemic therapy, other than metformin and DPP4's, for more than 14 days (consecutive or not) during the 12 weeks prior to screening, as well as previous exposure to DPP4 or SGLT-2 inhibitor in any DPP4 or SGLT-2 inhibitor trial is an exclusion criterion.
   16. Current treatment with potent cytochrome P450 3A4/5 inhibitors (in countries where dose adjustment would be required by the dapagliflozin label).
   17. Administration of any other investigational drug or participation in any interventional clinical studies within 30 days of planned screening to this study. Subjects who failed to satisfy all eligibility criteria at screening and did not enter the lead-in or open-label period in CV181-168 or CV181-169 studies specifically, do not need to wait 30 days.

3. Physical and Laboratory Test Findings

   a) Hemoglobin ≤ 11.0 g/dL (110 g/L) for men; hemoglobin ≤ 10.0 g/dL (100 g/L) for women

   b) Presence of hematuria:

   i) For male subjects being considered for Stratum A: microscopic hematuria present at Week -18 or Week -16 AND no common cause that can be confirmed is exclusionary. Male subjects with a confirmed common cause can be entered into the open-label phase with a documented negative result for hematuria microscopic urinalysis performed by the central laboratory.

   ii) For male subjects being considered for Stratum B: microscopic hematuria present at Week -10 or Week -8 AND no common cause that can be confirmed is exclusionary. Male subjects with a confirmed common cause can be entered into the open-label phase with a documented negative result for hematuria microscopic urinalysis performed by the central laboratory.

   NOTE: Female sub}ects with hematuria can be entered into the open-label phase and be randomized, but should be investigated according to local standards and best clinical practices. (See Appendix 3)

   c) Other central laboratory test findings:

   - Abnormal free T4 values. Abnormal thyroid stimulating hormone (TSH) value at screening will be further evaluated by free T4. Sub}ects with abnormal free T4 values will be excluded.

   • Positive for hepatitis B surface antigen
   • Positive for anti-hepatitis C virus antibody

4. Allergies and Adverse Drug Reaction

   a) Subjects who have contraindications to therapy as outlined in the dapagliflozin and saxagliptin Investigator Brochure, the local dapagliflozin or saxagliptin package insert or the local metformin package insert, including current treatment with potent cytochrome P450 3A4/5 inhibitors (in countries where dose adjustment would be required by the local Onglyza (saxagliptin) label.

5. Sex and Reproductive Status

   a) Women who are pregnant

6. Other Exclusion Criteria

   1. Prisoners or subjects who are involuntarily incarcerated
   2. Subjects who are compulsorily detained for treatment of either a psychiatric or physical (eg, infectious disease) illness
   3. Subjects on a commercial weight loss program with ongoing weight loss, or on an intensive exercise program.
   4. Employee of BMS, AstraZeneca (AZ), or their relatives.
   5. Subject with any condition which, in the judgment of the Investigator, may render the subject unable to complete the study or which may pose a significant risk to the subject.

   6. Subject is a participating investigator, study coordinator, employee of an investigator or immediate family member of any of the aforementioned.

      Open Label Treatment Period

      Note: Enrollment of subjects into the open-label (Stratum A) treatment period, beginning eee -16 of the study with HbA1c values at the lower bound (≥ 8.0% and ≤ 9.0%) and Enrollment of subjects into the open-label (Stratum B) eee -8, of the study with HbA1c values at the lower bound (≥ 7.5% and ≤ 8.5%) will be limited to approximately 50% of the total number of subjects randomized.

      • For subject in Stratum A:

      • At Week -10 and Week -2 a FPG qualification check will be performed. Subjects with a central laboratory FPG value meeting > 270 mg/dL will be scheduled for a follow-up visit (within 3 - 5 days) to obtain a second central laboratory FPG value. If the mean of the originally scheduled central laboratory FPG and the repeat central laboratory FPG value is > 270 mg/dL, the subject cannot be randomized and must be discontinued.

        • For subjects in Stratum B:
      • At Week -2 a FPG qualification check will be performed. Subjects with a central laboratory FPG value meeting > 270 mg/dL will be scheduled for a follow-up visit (within 3 - 5 days) to obtain a second central laboratory FPG value. If the mean of the originally scheduled central laboratory FPG and the repeat central laboratory FPG value is > 270 mg/dL, the subject cannot be randomized and must be discontinued

      Double Blind Treatment Period

      Inclusion criteria:

      • For Stratum A AND Stratum B:

      - Subjects with T2DM with inadequate glycemic control, defined as central laboratory HbA1c ≥ 7.0 and ≤ 10.5% obtained at the Week -2 visit of the open-label treatment period.

Study Plan

How is the study designed?

Design Details

• Primary Purpose: Treatment
• Allocation: Randomized
• Interventional Model: Parallel Assignment
• Masking: Triple

Number of Arms

2

Arms and Interventions

Participant Group / Arm	Intervention / Treatment
Experimental: Arm1: Dapagliflozin (10 mg) + Saxagliptin + Metformin IR	Drug: Dapagliflozin; Tablets, Oral, 10 mg, Once daily, Up to 52 weeks; Drug: Saxagliptin; Tablets, Oral, 5 mg, Once daily, Up to 52 weeks; Other Names: Onglyza; Drug: Metformin immediate release (IR); Tablets, Oral, ≥ 1500 mg, Twice daily, Up to 52 weeks
Experimental: Arm 2: Placebo + Saxagliptin + Metformin IR	Drug: Saxagliptin; Tablets, Oral, 5 mg, Once daily, Up to 52 weeks; Other Names: Onglyza; Drug: Metformin immediate release (IR); Tablets, Oral, ≥ 1500 mg, Twice daily, Up to 52 weeks; Drug: Placebo matching with Dapagliflozin; Tablets, Oral, 0 mg, Once daily, Up to 52 weeks

What is the study measuring?

Primary Outcome Measures

Outcome Measure	Measure Description	Time Frame
Adjusted Mean Change From Baseline in Hemoglobin A1C (HbA1c) at Week 24	HbA1c was measured as percent of hemoglobin by a central laboratory. Data after rescue medication was excluded from this analysis. Baseline was defined as the last assessment prior to the start date and time of the first dose of the double-blind study medication. In cases where time of the first dose or time of the assessment was not available, baseline was defined as the last assessment on or prior to the date of the first dose of the double-blind study medication. HbA1c measurements were obtained during the qualification and lead-in periods and on Day 1 and Weeks 6, 12, 18, and 24 in the double-blind period.	From Baseline to Week 24

Secondary Outcome Measures

Outcome Measure	Measure Description	Time Frame
Adjusted Mean Change From Baseline in Fasting Plasma Glucose at Week 24	Data after rescue medication was excluded from this analysis. Baseline was defined as the last assessment prior to the start date and time of the first dose of the double-blind study medication. In cases where time of the first dose or time of the assessment was not available, baseline was defined as the last assessment on or prior to the date of the first dose of the double-blind study medication. FPG measurements were obtained during the qualification and lead-in periods and on Day 1 and Weeks 6, 12, 18, and 24 in the double-blind period	From Baseline to Week 24
Adjusted Mean Change From Baseline in 120-minute Postprandial Glucose (PPG) at Week 24	2-hour postprandial glucose (PPG) from a liquid meal tolerance test (2-h MTT) Subject must be fasted for at least 8 hrs prior to the MTT.	From Baseline to Week 24
Adjusted Mean Change From Baseline in Body Weight at Week 24	Data after rescue medication was excluded from this analysis. Baseline was defined as the last assessment prior to the start date and time of the first dose of the double-blind study medication. In cases where time of the first dose or time of the assessment was not available, baseline was defined as the last assessment on or prior to the date of the first dose of the double-blind study medication. Body weights were measured during the qualification and lead-in periods and on Day 1 and Weeks 6, 12, 18, and 24 in the double-blind period.	From baseline to Week 24
Percentage of Subjects Achieving a Therapeutic Glycemic Response (Hemoglobin A1c [HbA1C]) <7.0% at Week 24 (Last Observation Carried Forward [LOCF])	Percent adjusted for baseline HbA1c. Therapeutic glycemic response is defined as HbA1c <7.0%. Data after rescue medication was excluded from this analysis.	From baseline to week 24

Collaborators and Investigators

• Sponsor: AstraZeneca

Publications and helpful links

General Publications

• Mathieu C, Catrinoiu D, Ranetti AE, Johnsson E, Hansen L, Chen H, Garcia-Sanchez R, Iqbal N, Celinski A. Characterization of the Open-Label Lead-In Period of Two Randomized Controlled Phase 3 Trials Evaluating Dapagliflozin, Saxagliptin, and Metformin in Type 2 Diabetes. Diabetes Ther. 2018 Aug;9(4):1703-1711. doi: 10.1007/s13300-018-0445-x. Epub 2018 May 25.
• Mathieu C, Ranetti AE, Li D, Ekholm E, Cook W, Hirshberg B, Chen H, Hansen L, Iqbal N. Randomized, Double-Blind, Phase 3 Trial of Triple Therapy With Dapagliflozin Add-on to Saxagliptin Plus Metformin in Type 2 Diabetes. Diabetes Care. 2015 Nov;38(11):2009-17. doi: 10.2337/dc15-0779. Epub 2015 Aug 5.

Helpful Links

• BMS clinical trial educational resource

Study record dates

Study Major Dates

• Study Start: September 1, 2012
• Primary Completion (Actual): August 1, 2014
• Study Completion (Actual): February 1, 2015

Study Registration Dates

• First Submitted: July 18, 2012
• First Submitted That Met QC Criteria: July 19, 2012
• First Posted (Estimate): July 20, 2012

Study Record Updates

• Last Update Posted (Estimate): June 22, 2016
• Last Update Submitted That Met QC Criteria: May 18, 2016
• Last Verified: May 1, 2016

More Information

Terms related to this study

• Additional Relevant MeSH Terms: Glucose Metabolism Disorders; Metabolic Diseases; Endocrine System Diseases; Diabetes Mellitus; Diabetes Mellitus, Type 2; Hypoglycemic Agents; Physiological Effects of Drugs; Molecular Mechanisms of Pharmacological Action; Enzyme Inhibitors; Hormones; Hormones, Hormone Substitutes, and Hormone Antagonists; Protease Inhibitors; Incretins; Sodium-Glucose Transporter 2 Inhibitors; Dipeptidyl-Peptidase IV Inhibitors; Dapagliflozin; Metformin; Saxagliptin
• Other Study ID Numbers: MB102-129; 2011-006324-20 (EudraCT Number)