Prospective Trial Comparing Conventional Versus Piggyback Method in Venous Drainage of the Transplanted Liver (LTx-outflow)

Prospective Randomized Trial Comparing the Clinical Efficiency of Conventional Versus Piggyback Method in Venous Drainage of the Transplanted Liver

METHODS: Patients were submitted to conventional (n=15) or piggyback (n=17) liver transplantation (LTx). Free hepatic vein pressure (FHVP) and the central venous pressure (CVP) measurements were performed after graft reperfusion. Postoperative (PO) serum creatinine (Cr) was measured, acute renal failure (ARF) was defined as Cr > or = 2,0mg/dL and PO renal function was analyzed by modificated RIFLE-AKIN. PO overall Cr was calculated by area under the curve (AUC) of Cr vs. time. on the first week.

Study Overview

• Status: Completed
• Conditions: End-stage Liver Disease
• Intervention / Treatment: Procedure: conventional method; Procedure: Piggyback method

Detailed Description

Thirty-two patients recruited among liver transplant recipients submitted to surgery at the Liver Unit at the Hospital das Clínicas of the University of São Paulo Medical School between 8 th October, 1999 and 30 rd September, 2000 were studied. The patients were assigned by randomization to two groups: transplant performed using the conventional or the piggyback method. These patients are part of a larger clinical trial where pulmonary alterations are compared between patients operated using the conventional method with venovenous bypass and the piggyback method.(10) Inclusion criteria admitted of both genders, aged 18 years or older, submitted to a first elective LTx, with no clinical or technical reasons justifying a preferential option by conventional or piggyback method. For this reason, we excluded patients submitted to living donor LTx, in whom IVC is routinely preserved and those with familial amyloidotic polyneuropathy, whom, in our routine, are routinely submitted to conventional LTx.

The randomization process was performed by a team of nurses not involved with the intraoperative care of the patients. This was obtained by "coin-tossing". In each case, randomization was paired according to the Child-Pugh's Score, calculated immediately before surgery. Stratification was performed by blocking randomization in each subset of patient (scores A, B, or C), with blocks of size 2 with a one-to-one allocation ratio. Thus, while the first patient of each Child-Pugh score was effectively randomized, the second was automatically allocated to the opposite surgical method, completing a pair. However, the investigators had no influence on the second recipient selection due to the characteristics of the organ allocation system in Brazil, former MELD scale criteria was introduced. In that time, the graft distribution was according to four distinct blood groups (A,B,AB,O) generated a greater obstacle to predicting who would be the next patient as related to what would be obtained if blocks of 4 size have been used.

Surgeries of donor and of recipient were performed with small adaptations in the usual technique in order to evaluate the pressure gradient between the hepatic vein and the right atrium in the two groups. In the conventional method IVC was clamped during the anhepatic phase and venous return was maintained by a portal femoral axillary venovenous bypass with a centrifugal pump. In these cases, IVC reconstruction was performed by end-to-end anastomosis above and below the liver. In the piggyback method IVC was not clamped in any case. Implantation method of the grafted IVC in recipient IVC was not standardized, being defined by the responsible surgeon during the procedure. In the two groups, all patients were submitted to simultaneous arterial and portal revascularization, according to the routine of the service.

Free hepatic vein pressure (FHVP) was measured using an 8F polyethylene catheter with a multiperforated distal end, which was positioned in the graft's right hepatic vein during ex situ preparation on the back table. The proximal end of this catheter was exteriorized in the infrahepatic portion of IVC. In the conventional group, exteriorization was performed through the anastomosis suture. In the piggyback group, IVC was ligated around the catheter. Central venous pressure (CVP) was obtained using a Swan-Ganz catheter (routine procedure). Measurement of hepatic vein and right atrium pressure was made once, after concluding biliary anastomosis.

All measurements were obtained with the same transducer, determining as zero reference level the median axillary line. Pressure measurement was performed in apnea in order to avoid that the patient's respiratory incursions would determine oscillations in the pressure curve. When oscillations persisted in spite of this maneuver, the arithmetic mean of the observed maximum and minimum values was recorded. "hepatic venous block" was considered when a pressure gradient higher than 3 mm Hg was present.(4) Serum creatinine (Cr) was determined in the preoperatory period (immediately before surgery), on postoperative days (PO) 1 to 7 and on 14, 21 and 28. Occurrence of acute renal failure (ARF) was defined as postoperative creatinine peak > or = 2.0. (8) For each case, the area under the curve (AUC) Cr vs. time, calculated by the trapezoidal method (11) and divided by the postoperative observation time (28 days), was used as a postoperative overall creatinine parameter. Each group, conventional and piggyback, are also analyzed according to the RIFLE which classifies ARF in three grades of increasing severity: risk (class R), injury (class I) and failure (class F) - and two outcome classes - loss and end-stage kidney disease. RIFLE classification provides these grades based on changes in either Cr or urine output from the baseline condition. The RIFLE criteria is an important tool to help the international community compare data on ARF and was validated elsewhere (12, 13), being modified in 2005 as RIFLE-AKIN (14). For statistical analysis, in this study, the Class R are unified with the group of patients without renal injuries (considered as nule alterations), and the patients with class I and F were unified. The outcome classes are late consequences of the LTx and were not studied in the present clinical trial.

Postoperative massive ascites was considered as abdominal fluid accumulation with a volume over 500 ml/day for more than 30 days (6) evaluated through body weight, abdominal drain output or paracentesis.

The patients were followed up until 30 rd June, 2006, recording the date of death of those who died.

• Study Type: Interventional
• Enrollment (Actual): 32
• Phase: Not Applicable

Participation Criteria

Eligibility Criteria

• Ages Eligible for Study: 14 years and older (Adult, Older Adult)
• Accepts Healthy Volunteers: No
• Genders Eligible for Study: All

Description

Inclusion Criteria:

• Inclusion criteria admitted of both genders, aged 18 years or older, submitted to a first elective LTx, with no clinical or technical reasons justifying a preferential option by conventional or piggyback method

Exclusion Criteria:

• patients submitted to living donor LTx, in whom IVC is routinely preserved and those with familial amyloidotic polyneuropathy, whom, in our routine, are routinely submitted to conventional LTx.

Study Plan

How is the study designed?

Design Details

• Primary Purpose: Treatment
• Allocation: Randomized
• Interventional Model: Parallel Assignment
• Masking: None (Open Label)

Number of Arms

2

Arms and Interventions

Participant Group / Arm	Intervention / Treatment
Sham Comparator: Conventional method; In the conventional method IVC was clamped during the anhepatic phase and venous return was maintained by a portal femoral axillary venovenous bypass with a centrifugal pump. In these cases, IVC reconstruction was performed by end-to-end anastomosis above and below the liver.	Procedure: conventional method
Experimental: Piggyback method; In the piggyback method IVC was not clamped in any case. Implantation method of the grafted IVC in recipient IVC was not standardized, being defined by the responsible surgeon during the procedure. In the two groups, all patients were submitted to simultaneous arterial and portal revascularization, according to the routine of the service.	Procedure: Piggyback method; In the piggyback method IVC was not clamped in any case. Implantation method of the grafted IVC in recipient IVC was not standardized, being defined by the responsible surgeon during the procedure. In the two groups, all patients were submitted to simultaneous arterial and portal revascularization, according to the routine of the service.; Other Names: Liver transplantaton piggyback method

What is the study measuring?

Primary Outcome Measures

Outcome Measure	Measure Description	Time Frame
Hepatic vein and right atrium pressure measurements (observed FHPV-CVP gradient values)	Free hepatic vein pressure (FHVP) was measured using an 8F polyethylene catheter with a multiperforated distal end, which was positioned in the graft's right hepatic vein during ex situ preparation on the back table. The proximal end of this catheter was exteriorized in the infrahepatic portion of IVC. In the conventional group, exteriorization was performed through the anastomosis suture. In the piggyback group, IVC was ligated around the catheter. Central venous pressure (CVP) was obtained using a Swan-Ganz catheter (routine procedure). Measurement of hepatic vein and right atrium pressure was made once, after concluding biliary anastomosis.	intraoperatory

Secondary Outcome Measures

Outcome Measure	Measure Description	Time Frame
Serum Creatinine	Serum creatinine (Cr) was determined in the preoperatory period (immediately before surgery), on postoperative days (PO) 1 to 7 and on 14, 21 and 28. Occurrence of acute renal failure (ARF) was defined as postoperative creatinine peak > or = 2.0	postoperative days (PO) 1 to 7 and on 14, 21 and 28

Collaborators and Investigators

• Sponsor: University of Sao Paulo
• Collaborators: Fundação de Amparo à Pesquisa do Estado de São Paulo
• Investigators: Study Chair: Paulo CB Massarollo, PhD, Faculdade de Medicina da Universidade de Sao Paulo

Publications and helpful links

General Publications

• Brescia MD, Massarollo PC, Imakuma ES, Mies S. Prospective Randomized Trial Comparing Hepatic Venous Outflow and Renal Function after Conventional versus Piggyback Liver Transplantation. PLoS One. 2015 Jun 26;10(6):e0129923. doi: 10.1371/journal.pone.0129923. eCollection 2015.

Study record dates

Study Major Dates

• Study Start: October 1, 1999
• Primary Completion (Actual): September 1, 2000
• Study Completion (Actual): October 1, 2000

Study Registration Dates

• First Submitted: October 12, 2012
• First Submitted That Met QC Criteria: October 15, 2012
• First Posted (Estimate): October 16, 2012

Study Record Updates

• Last Update Posted (Estimate): October 16, 2012
• Last Update Submitted That Met QC Criteria: October 15, 2012
• Last Verified: October 1, 2012

More Information

Terms related to this study

• Keywords: Liver transplatation
• Additional Relevant MeSH Terms: Digestive System Diseases; Liver Failure; Hepatic Insufficiency; Liver Diseases; End Stage Liver Disease
• Other Study ID Numbers: LIVER TRANSPLANT OUTFLOW