Tenofovir Levels Following Local Application of Tenofovir Reduced-Glycerin 1% Gel

A Phase 1 Crossover Trial Evaluating the Pharmacokinetics of Tenofovir Reduced-Glycerin 1% Gel in the Rectal and Vaginal Compartments in Women

To compare local and systemic pharmacokinetics of tenofovir reduced-glycerin (TFV RG) 1% gel after 2 weeks of daily rectal use and after 2 weeks of daily vaginal use

Study Overview

• Status: Completed
• Conditions: HIV Prevention
• Intervention / Treatment: Drug: Once Daily Application of TFV RG 1% Gel - Vaginal; Drug: Once Daily Application of TFV RG 1% gel - Rectal

• Study Type: Interventional
• Enrollment (Actual): 14
• Phase: Phase 1

Contacts and Locations

Study Locations

• United States
  • New York
    • New York, New York, United States, 10032
      • Center for Infectious Disease Epidemiologic Research, Mailman School of Public Health, Columbia University

Participation Criteria

Eligibility Criteria

• Ages Eligible for Study: 21 years to 45 years (Adult)
• Accepts Healthy Volunteers: Yes
• Genders Eligible for Study: Female

Description

Inclusion Criteria:

1. Age 21 through 45 years (inclusive) at Screening
2. Able and willing to provide written informed consent
3. Able and willing to comply with all study procedure requirements, including, clinical and laboratory assessments, vaginal and rectal examinations, urine and blood testing, as well as attendance at all scheduled study visits
4. In general good health at Screening and Enrollment as determined by the Investigator of Record (IoR)/ or designee
5. Negative pregnancy test at Screening and Enrollment
6. HIV-negative at Screening and Enrollment
7. Able and willing to provide adequate locator information
8. Willingness to use study-provided male condoms for the duration of study participation for penetrative intercourse
9. Per participant report at Screening, regular menstrual cycles with at least 21 days between menses (does not apply to participants who report using a progestin-only method of contraception at screening, e.g., Depo-Provera, progesterone-containing IUDs or extended use of oral contraceptives)

10. Per participant report at Enrollment, using an effective method of contraception and intending to use an effective method for the duration of study participation; effective methods include:

    • Hormonal methods, excluding vaginal rings
    • Intrauterine device (IUD) inserted at least 42 days prior to Enrollment (but not past the maximum length of recommended usage according to package instructions)
    • Sterilization of participant or partner at least 42 days prior to Enrollment
    • Self-identifies as a woman who has sex with women exclusively
    • Sexually abstinent for the at least 90 days prior to enrollment and the intention to remain sexually abstinent for the duration of study participation
11. Per participant report at Screening, states a willingness to refrain from inserting any non-study vaginal or rectal products or objects into the vagina or rectum, including but not limited to, spermicides, female condoms, diaphragms, contraceptive vaginal rings, vaginal medications, menstrual cups, cervical caps (or any other vaginal barrier method), vaginal/rectal douches, enemas, non-study approved lubricants, sex toys (vibrators, dildos, etc.), and tampons for the duration of the study product use periods and for 24 hours prior to each scheduled study clinic visit.
12. Pap result consistent with Grade 0 according to the Female Genital Grading Table for Use in Microbicide Studies Addendum 1 to the Division of AIDS (DAIDS) Table for Grading the Severity of Adult and Pediatric Adverse Events, Version 1.0, December 2004 (Clarification dated August 2009) or satisfactory evaluation of non-Grade 0 Pap result with no treatment required per clinical judgment of IoR or designee in the 12 calendar months prior to the Enrollment Visit

13. At Screening, participant agrees not to take part in other research studies involving drugs, medical devices, or vaginal/rectal products for the duration of study participation (including the time between the Screening and Enrollment visits)

    Participants in the biopsy subset must also meet the following criteria at Screening to be eligible for inclusion:

14. Willing to abstain from inserting anything into the vagina or rectum for 72 hours prior to and following the collection of these samples, including vaginal and rectal intercourse
15. Willing to restrict the use of non-steroidal anti-inflammatory drugs (NSAIDs), aspirin and/or other drugs that are associated with the increased likelihood of bleeding following mucosal biopsy collection for 72 hours prior to and following the collection biopsies

Exclusion Criteria:

1. Participant report of any of the following:

   1. Known adverse reaction to the study product (ever)
   2. Known adverse reaction to latex (ever)
   3. Current male sex partner with known history of adverse reaction to latex (ever)
   4. History of serum HBsAg positivity (ever)
   5. Non-therapeutic injection drug use in the 12 calendar months prior to Enrollment
   6. Sexually transmitted infections (STI) or reproductive tract infection (RTI) requiring treatment in the 6 calendar months prior to Enrollment
   7. Post-exposure prophylaxis (PEP) for possible HIV-1 infection within the 6 calendar months prior to Enrollment
   8. Last pregnancy outcome within 90 days or less prior to Enrollment
   9. Gynecologic or genital procedure (e.g., tubal ligation, dilation and curettage) within the 42 days prior to Enrollment Note: This does not include biopsy for the evaluation of an abnormal pap result or endometrial biopsy that occurred more than 7 days prior to Enrollment, provided that all other inclusion/exclusion criteria are met.
   10. Participation in any other research study involving drugs, medical devices or vaginal products 42 days or less prior to Enrollment
   11. Anticipated IUD replacement within the next 3 months or an IUD inserted 42 days or less prior to Enrollment
   12. Participant report at Screening and/or Enrollment intention of becoming pregnant in the next 3 months
   13. Currently breastfeeding at the time of Screening and/or Enrollment
   14. History of bleeding problems (Participants in the biopsy subset only)

2. Laboratory abnormalities at Screening greater than or equal to a Grade 2*:

   1. Aspartate aminotransferase (AST) or alanine transaminase (ALT)
   2. Hemoglobin
   3. Platelet count
   4. Serum creatinine Otherwise eligible participants with an exclusionary test result(s) listed above may be re-tested during the screening process. If a participant is re-tested and a non-exclusionary result is documented within the 42 days of providing informed consent, the participant may be enrolled.
3. Urinary tract infection (UTI) at Screening and/or Enrollment Note: Otherwise eligible participants diagnosed with UTI during Screening will be offered treatment and may be enrolled after completing treatment and all symptoms have resolved. If treatment is completed and symptoms have resolved within 42 days of obtaining informed consent, the participant may be enrolled.
4. Pelvic inflammatory disease or an STI or RTI requiring treatment per current World Health Organization (WHO) guidelines at Screening and/or Enrollment

5. Clinically apparent Grade 2 or higher pelvic** and/or rectal*** examination finding (observed by study staff) at Screening and/or Enrollment Note: Cervical bleeding associated with speculum insertion and/or specimen collection judged to be within the range of normal according to the clinical judgment of the IoR/designee is considered expected non-menstrual bleeding and is not exclusionary.

   Note: Otherwise eligible participants with exclusionary pelvic and/or rectal examination findings may be enrolled/randomized after the findings have improved to a non-exclusionary severity grading or resolved. If improvement to a non-exclusionary grade or resolution is documented within 42 days of providing informed consent, the participant may be enrolled.

6. Any other condition that, in the opinion of the IoR/designee, would preclude informed consent, make study participation unsafe, complicate interpretation of study outcome data, or otherwise interfere with achieving the study objectives

   • per the Division of AIDS Table for Grading the Severity of Adult and Pediatric Adverse Events Version 1.0, December, 2004 (Clarification dated August 2009) ** per the Division of AIDS Table for Grading the Severity of Adult and Pediatric Adverse Events Version 1.0, December, 2004 (Clarification dated August 2009), Addendum 1 Female Genital Grading Table for Use in Microbicide Studies *** per the Division of AIDS Table for Grading the Severity of Adult and Pediatric Adverse Events Version 1.0, December, 2004 (Clarification dated August 2009), Addendum 3 Rectal Grading Table for Use in Microbicide Studies (Clarification dated May 2012).

Study Plan

How is the study designed?

Design Details

• Primary Purpose: Other
• Allocation: Randomized
• Interventional Model: Crossover Assignment
• Masking: None (Open Label)

Number of Arms

2

Arms and Interventions

Participant Group / Arm	Intervention / Treatment
Active Comparator: Sequence A; 2 weeks, 6 week washout, 2 weeks	Drug: Once Daily Application of TFV RG 1% Gel - Vaginal; Vaginal application; Drug: Once Daily Application of TFV RG 1% gel - Rectal; Rectal application
Active Comparator: Sequence B; 2 weeks, 6 week washout, 2 weeks	Drug: Once Daily Application of TFV RG 1% Gel - Vaginal; Vaginal application; Drug: Once Daily Application of TFV RG 1% gel - Rectal; Rectal application

What is the study measuring?

Primary Outcome Measures

Outcome Measure	Measure Description	Time Frame
Drug concentrations	To compare local and systemic pharmacokinetics of tenofovir reduced-glycerin 1% gel after 2 weeks of daily rectal use and after 2 weeks of daily vaginal use	10 weeks

Secondary Outcome Measures

Outcome Measure	Measure Description	Time Frame
Number of adverse events Grade 2 or higher	To assess the safety of tenofovir reduced-glycerin 1% gel after 2 weeks of daily rectal use and after 2 weeks of daily vaginal use	10 weeks

Other Outcome Measures

Outcome Measure	Measure Description	Time Frame
Inhibition of HIV by study drug in rectal and genital fluids	Correlate drug levels in rectal and genital fluids with drug potency	10 weeks
Microflora biomarkers and gene expression from the vaginal and rectal environments	Determine changes in microflora biomarkers and gene expression from the vaginal and rectal environments after 2 weeks of daily rectal versus 2 weeks of daily vaginal use of tenofovir reduced-glycerin 1% gel at sites with capacity	10 weeks

Collaborators and Investigators

• Sponsor: CONRAD
• Collaborators: National Institute of Allergy and Infectious Diseases (NIAID); Eunice Kennedy Shriver National Institute of Child Health and Human Development (NICHD); National Institute of Mental Health (NIMH)
• Investigators: Principal Investigator: Jessica Justman, MD, Columbia University; Study Chair: Gonasagrie Nair, MBChB, Centre for the AIDS Programme of Research in South Africa (CAPRISA) - eThekwini CRS

Publications and helpful links

General Publications

• Justman JE, Nair GL, Hendrix CW, Piper JM, Marzinke MA, Dai JY, Pan Z, Galaska B, Levy L, Schwartz JL, Balar B, Kunjara Na Ayudhya RP, Mushamiri I, McGowan I, Dezzutti CS; MTN-014 Study Team. Pharmacokinetics and Pharmacodynamics of Tenofovir Reduced-Glycerin 1% Gel in the Rectal and Vaginal Compartments in Women: A Cross-Compartmental Study With Directly Observed Dosing. J Acquir Immune Defic Syndr. 2018 Jun 1;78(2):175-182. doi: 10.1097/QAI.0000000000001655.

Helpful Links

• Related Info

Study record dates

Study Major Dates

• Study Start: April 1, 2014
• Primary Completion (Actual): December 1, 2014
• Study Completion (Actual): December 1, 2014

Study Registration Dates

• First Submitted: November 20, 2012
• First Submitted That Met QC Criteria: January 14, 2013
• First Posted (Estimate): January 16, 2013

Study Record Updates

• Last Update Posted (Actual): June 28, 2021
• Last Update Submitted That Met QC Criteria: June 22, 2021
• Last Verified: June 1, 2021

More Information

Terms related to this study

• Keywords: Pharmacokinetics; Microbicides; Tenofovir; Rectal use; Reduced-glycerin; Vaginal use
• Other Study ID Numbers: MTN-014; 5UM1AI068633 (U.S. NIH Grant/Contract); 11885 (Other Grant/Funding Number: DAIDS Protocol Number)