Effect of a Single Dose of Cranberry Beverage on Inflammation and Oxidative Stress

Acute Bioavailability of Cranberry Polyphenols After A Single Dose In Healthy Adults and Its Antioxidative and Anti-Inflammatory Action

The purpose of this study is to determine the effects of a single dose of cranberry beverage in healthy adults on the kinetic uptake of polyphenol compounds and polyphenol metabolites, and antioxidative and anti-inflammatory activity.

Study Overview

• Status: Completed
• Conditions: Inflammation
• Intervention / Treatment: Other: Low Calorie Cranberry Juice Cocktail; Other: Cranberry Extract Beverage; Other: Non-Cranberry Beverage

Detailed Description

Participants will be randomized based on age, sex and BMI, and will consume a low-polyphenol diet 2 days prior to the intervention. On the day of the study, baseline blood and urine samples will be collected after an overnight fast. Participants will consume the assigned beverage within 15 minutes and blood collected at 2, 4, 8, and 24 h (10 mL/blood draw; total blood volume per period = 50 mL). Urine will be collected for polyphenol analysis during the following periods: 0-3, 3-6, 6-9, 9-12, 12-24 hours. After a one-week washout period, participants will receive their second and then the third beverage in a cross-over design; blood and urine sampling will be repeated at each of the 3 intervention periods.

• Study Type: Interventional
• Enrollment (Actual): 12
• Phase: Not Applicable

Contacts and Locations

Study Locations

• United States
  • Washington
    • Pullman, Washington, United States, 99164
      • WSU Bioactives Research Lab

Participation Criteria

Eligibility Criteria

• Ages Eligible for Study: 18 years to 35 years (ADULT)
• Accepts Healthy Volunteers: Yes
• Genders Eligible for Study: All

Description

Inclusion Criteria:

• Men & women; aged 18-35 years
• BMI: 19-25 kg/m2

Exclusion Criteria:

• Any significant chronic disease, hypertension, ulcers, dyspepsia, lactose intolerance, allergy, psychotic illness
• Had major gastrointestinal surgery or on prescription or surgical treatment
• Any condition that may interfere with the digestion, absorption, metabolism or excretion of nutrients
• Regularly smoked in the previous 6 months
• On medication or ingested a prescribed drug at any time within the 14 days preceding study enrolment (excluding hormonal contraceptives and hormone replacement therapy) or over-the-counter preparation within 7 days preceding enrolment
• Risk factors for AIDS or known HIV positive status
• Pregnant or lactating
• High intake of chocolate or similar high polyphenol foods
• Regular intake of vitamin and polyphenol supplements
• Drug and alcohol misuse
• Currently participating or had participated in another clinical study during the previous 3 weeks
• Donated blood in the previous 3 weeks
• Exercise more than 3 times/week for longer than 45 min each time

Study Plan

How is the study designed?

Design Details

• Primary Purpose: BASIC_SCIENCE
• Allocation: RANDOMIZED
• Interventional Model: CROSSOVER
• Masking: DOUBLE

Number of Arms

3

Arms and Interventions

Participant Group / Arm	Intervention / Treatment
ACTIVE_COMPARATOR: Low Calorie Cranberry Juice Cocktail; Beverage containing cranberry: one dose of 15.2 ounces consumed within 15 minutes	Other: Low Calorie Cranberry Juice Cocktail; Beverage containing cranberry at a dose of 15.2 ounces consumed within 15 minutes
ACTIVE_COMPARATOR: Cranberry Extract Beverage; Beverage containing cranberry: one dose of 15.2 ounces consumed within 15 minutes	Other: Cranberry Extract Beverage; Beverage containing cranberry at a dose of 15.2 ounces consumed within 15 minutes
PLACEBO_COMPARATOR: Non-Cranberry Beverage; Beverage absent cranberry: one dose of 15.2 ounces consumed within 15 minutes	Other: Non-Cranberry Beverage; Beverage absent cranberry at a dose of 15.2 ounces consumed within 15 minutes

What is the study measuring?

Primary Outcome Measures

Outcome Measure	Time Frame
Reduced Glutathione (GSH) Concentrations in Red Blood Cells	0, 2, 4, 8, 24 h
Superoxide Dismutase (SOD) Activity in Red Blood Cells	0, 2, 4, 8, 24 h
Glutathione Peroxidase (GPx) Activity in Red Blood Cells	0, 2, 4, 8, 24 h
Oxidative Damage to DNA Assessed by Plasma 8-hydroxy-2'-Deoxyguanosine (8-OHdG)	0, 2, 4, 8, 24 h
C-Reactive Protein (CRP) Concentrations in Plamsa	0, 2, 4, 8, 24 h
Nitric Oxide (NO) Concentrations in Plamsa	0, 2, 4, 8, 24 h
Interleukin-1alpha (IL-1a) Concentrations in Plasma	0, 2, 4, 8, 24 h
Interleukin-1beta (IL-1b) Concentrations in Plasma	0, 2, 4, 8, 24 h
Interleukin-2 (IL-2) Concentrations in Plasma	0, 2, 4, 8, 24 h
Interleukin-4 (IL-4) Concentrations in Plasma	0, 2, 4, 8, 24 h
Interleukin-6 (IL-6) Concentrations in Plasma	0, 2, 4, 8, 24 h
Interleukin-8 (IL-8) Concentrations in Plasma	0, 2, 4, 8, 24 h
Interleukin-10 (IL-10) Concentrations in Plasma	0, 2, 4, 8, 24 h
Interferon-gamma (IFN-y) Concentrations in Plasma	0, 2, 4, 8, 24 h
Tumor Necrosis Factor-alpha (TNF-a) Concentrations in Plasma	0, 2, 4, 8, 24 h
Urinary Anti-bacteria Adhesion Activity	0, 3, 6, 9, 12, 24 h

Collaborators and Investigators

• Sponsor: Washington State University
• Collaborators: Ocean Spray Cranberries, Inc.
• Investigators: Principal Investigator: Boon P Chew, PhD, Washington State University

Study record dates

Study Major Dates

• Study Start: July 1, 2010
• Primary Completion (ACTUAL): October 1, 2010
• Study Completion (ACTUAL): December 1, 2010

Study Registration Dates

• First Submitted: January 29, 2013
• First Submitted That Met QC Criteria: January 29, 2013
• First Posted (ESTIMATE): January 31, 2013

Study Record Updates

• Last Update Posted (ESTIMATE): July 8, 2014
• Last Update Submitted That Met QC Criteria: June 4, 2014
• Last Verified: June 1, 2014

More Information

Terms related to this study

• Keywords: Inflammation; Oxidative Stress; Vaccinium macrocarpon
• Additional Relevant MeSH Terms: Pathologic Processes; Inflammation
• Other Study ID Numbers: 11369